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作物学报 ›› 2013, Vol. 39 ›› Issue (07): 1214-1222.doi: 10.3724/SP.J.1006.2013.01214

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

甘蓝型油菜籽粒含油量、蛋白质、纤维素及半纤维素含量QTL分析

马珍珍1,2,李加纳1,2,Bennjiamin WITTKOP3,Martin FRAUEN4,阎星颖1,2,刘列钊1,2,*,肖阳1,2   

  1. 1 西南大学农学与生物技术学院, 重庆 400716; 2重庆市油菜工程技术研究中心, 重庆 400716; 3Department of Plant Breeding, IFZ Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany, 4Norddeutsche Pflanzenzucht Hans-Georg Lembke KG, 24363 Hohenlieth, Germany
  • 收稿日期:2012-11-16 修回日期:2013-03-11 出版日期:2013-07-12 网络出版日期:2013-04-23
  • 通讯作者: 刘列钊, E-mail: liezhao2003@126.com, Tel: 023-68250701
  • 基金资助:

    本研究由国家自然科学基金项目(31171584)和重庆市自然科学基金项目(cstc2011jjA8000g)资助。

Analysis of QTLs for Oil, Protein, Cellulose and Hemicellulose Contents of Seeds in Brassica napus L.

MA Zhen-Zhen1,2,LI Jia-Na1,2,Benjiamin WITTKOP3,Martin FRAUEN4,YAN Xing-Ying1,2,LIU Lie-Zhao1,2,*,XIAO Yang1,2   

  1. 1 College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China; 2 Chongqing Engineering Research Center for Rapeseed, Chongqing 400716, China; 3 Department of Plant Breeding, IFZ Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany; 4 Norddeutsche Pflanzenzucht Hans-Georg Lembke KG, 24363 Hohenlieth, Germany
  • Received:2012-11-16 Revised:2013-03-11 Published:2013-07-12 Published online:2013-04-23
  • Contact: 刘列钊, E-mail: liezhao2003@126.com, Tel: 023-68250701

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摘要：

以甘蓝型黄籽油菜GH06和甘蓝型黑籽油菜P174为亲本，通过单粒法连续自交8代构建重组自交系群体，应用SSR标记绘制31个连锁群(LGs)的遗传连锁图谱，图谱总长1437.1 cM，相邻标记间的平均距离为3.89 cM。对4个不同环境下RIL8群体中每个株系籽粒含油量、蛋白质、纤维素和半纤维素含量进行了近红外分析，性状相关性表明含油量与其他3个性状均表现负相关，蛋白质含量与纤维素和半纤维素分别表现负相关和正相关。结合构建的遗传图谱采用复合区间作图法分析4个性状QTL，共检测到26个QTL，分布在N2、N3、N8、N9、N11、N13、N16和N17连锁群上，其中8个含油量QTL可解释表型变异的4.96%~21.83%；6个蛋白含量QTL，可解释表型变异的3.12%~14.28%；4个纤维素含量QTL，可解释表型变异的4.60%~17.29%；8个半纤维素含量QTL，可解释表型变异率的6.66%~16.68%。在N8上，发现有含油量QTL与半纤维素含量QTL重叠的区段。在N9上，发现有纤维素含量QTL与半纤维素含量QTL重叠的区段，上述2个区段重叠QTL加性效应方向相反。本研究认为油菜种子含油量、蛋白质、纤维素和半纤维素属于典型的数量性状，受环境影响较大，与这些QTL紧密相关的分子标记可为下一步分子标记辅助育种提供一定技术支撑。

关键词: 甘蓝型油菜, 含油量, 蛋白质, 纤维素, 半纤维素, QTL

Abstract:

In order to study oil, protein, cellulose and hemicellulose contents of rapeseed, recombinant inbred lines (RILs) were developed through successive selfing up to eight generations from the F2 of a cross between yellow seeded female parent GH06 and black seeded male parent P174. The SSR markers were used to construct the linkage map, containing 31 linkage groups with the total map length of 1437.1 cM and an average distance of 3.89 cM. We measured the RIL population seeds quality in four different environments, the result showed that the oil content was negatively correlated with other three traits, and there were a negative correlation between protein content and cellulose content and a positive correlation between protein content and hemicellulose content. Using the method of composite interval mapping (CIM) to analysis oil, protein, cellulose and hemicellulose contents, a total of 26 QTLs for affecting the four traits were detected in linkage groups of N2, N3, N8, N9, N11, N13, N16, and N17. Eight QTLs were detected for oil content and the contribution for phenotypic variation ranged from 4.96% to 21.83%, six QTLs were detected for protein content and the contribution for phenotypic variation ranged from 3.12% to 14.28%, four QTLs were detected for cellulose content and the contribution for the phenotypic variation ranged from 4.60% to 17.29%, eight QTLs were detected for hemicellulose content and the contribution for phenotypic variation ranged from 6.66% to 16.68%. Furthermore, overlapping regions were observed in linkage group N8 between QTLs of oil content and QTLs for hemicellulose content, andin linkage group N9 between QTLs of cellulose content and QTLs for hemicellulose content. These results suggested that oil, protein, cellulose and hemicellulose contents are typical quantitative traits. They are controlled by many minor effect genes and influenced by environment. Molecular markers linked with these QTLs will be useful for facilitating molecular marker-assisted breeding.

Key words: Brassica napus L., Oil content, Protein, Cellulose, Hemicellulose, QTL

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