欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2013, Vol. 39 ›› Issue (07): 1206-1213.doi: 10.3724/SP.J.1006.2013.01206

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

广东不同时期水稻主栽品种抗病基因同源序列多态性分析

唐志明1,*,陈建酉2,陆永法1,周华成1,莫雪娇2,毛兴学2,李晓方3,*   

  1. 1宁波市农业科学研究院作物研究所，浙江宁波 315040; 2广东省农业科学院水稻研究所，广东广州 510640；3长江大学农学院，湖北荆州 434025；
  • 收稿日期:2012-10-22 修回日期:2013-03-19 出版日期:2013-07-12 网络出版日期:2013-04-23
  • 通讯作者: 李晓方, E-mail:lixiaofang@163.com; 唐志明，E-mail: tzm2004@163.com
  • 基金资助:

    本研究由广东省自然科学基金重点项目(06200585)和宁波水稻育种创新团队项目(2009B21001)资助。

Temporal Changes in Resistance Gene Analog Polymorphism of Major Rice Varieties in Guangdong Province

TANG Zhi-Ming1,*,CHEN Jian-You2,LU Yong-Fa1,ZHOU Hua-Cheng1,MO Xue-Jiao2,MAO Xing-Xue2,LI Xiao-Fang3,*   

  1. 1 Crop Research Institute, Ningbo Academy of Agricultural Sciences, Ningbo 315040, China; 2 Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; 3 Agricultural College, Yangtze University, Jingzhou 434025, China
  • Received:2012-10-22 Revised:2013-03-19 Published:2013-07-12 Published online:2013-04-23
  • Contact: 李晓方, E-mail:lixiaofang@163.com; 唐志明，E-mail: tzm2004@163.com

RichHTML

PDF

1089

被引次数

2

摘要：

为掌握广东不同时期水稻主栽品种抗病基因同源序列(RGA)多态性变化，利用3对RGA标记分析了广东20世纪50年代初地方品种及1972、1980、1994和2008年主栽品种。按供试品种RGA-PCR指纹聚类，以相似系数0.81为标准将171份品种分为20个类群。这五类品种分别分布于11、7、4、8、11个类群中，它们的Nei遗传多样性指数分别是0.801、0.682、0.493、0.603和0.527。相对于地方品种，1972、1980和1994年品种RGA多态性低是由于品种类群少及品种在各类群中分布均衡性差，2008年品种RGA多态性低是由于该年大多数品种属于同一类群，品种在各类群中分布均衡性差。因此保持品种抗性多样性，既要保证丰富类群，又要确保品种在各类群中分布均衡。近年来品种抗性背景趋同化是广东抗性多样性降低的主要原因，今后在新品种的选育上应均衡利用不同抗源。

关键词: 水稻品种, 抗病基因同源序列, 多态性

Abstract:

In order to investigate the temporal diversity of resistance gene analogs (RGA) from major rice varieties in Guangdong, landraces from early 1950s and major varieties from 1972, 1980, 1994, and 2008 in Guangdong Province were selected and analyzed using three RGA primers. One hundred and seventy-one varieties were divided into twenty groups according to the DNA band data at the similarity index of 0.81. Landraces from early 1950s and varieties form 1972, 1980, 1994, and 2008 were distributed into eleven, seven, four, eight and eleven groups, with their Nei polymorphism index of 0.801, 0.682, 0.493, 0.603, and 0.527, respectively. The RGA polymorphism of landraces was the highest. Having a few group types and unevenly distributing in different groups were the reasons of low diversity for the varieties of 1972, 1980, and 1994, while the low diversity for varieties of 2008 resulted from that major varieties distributed in one group and unevenly distributed among different groups. Thus, having various groups and evenly distribution is necessary for maintaining the resistance diversity of varieties. In recent years, the resistance diversity of rice varieties in Guangdong decreased as major varieties had similar genetic background in resistance. Thus, different resistant resources should be utilized equally in future rice breeding.

Key words: Rice varieties, Resistance gene analog, Polymorphism

[1]Lai Z-R(赖真如), Zou S-F(邹寿发), Xu Q-F(徐起峰). The occurrence trend and integrated control of rice disease in Guangdong. Guangdong Agric Sci (广东农业科学), 1999, (1): 32–33 (in Chinese) 



[2]Commission of Agricultural Statistical Yearbook of Guangdong (广东农村统计年鉴编委会). Agricultural Statistical Yearbook of Guangdong (广东农村统计年鉴). Beijing: China Statistics Press, 1993–2009 (in Chinese)



[3]Zeng F-S(曾凡松), Xiang L-B(向礼波), Yang L-J(杨立军), Yang X-L(杨小林), Yang J-S(杨金松), Yu D-Z(喻大昭). Diversity analysis for resistance of 251 rice (Oryza sativa L.) varieties (lines) to rice blast disease. Acta Phytopathol Sin (植物病理学报), 2011, 41(4): 399–410 (in Chinese with English abstract)



[4]Zhang C-H(张朝红), Liu M-J(刘孟军), Kong D-C(孔得仓), Wu G-L(吴国林), Li D-K(李登科). Diversification of resistance to fruit shrink disease in Chinese jujube germplasm. J Plant Genet Resour (植物遗传资源学报), 2011, 12(4): 539–545 (in Chinese with English abstract)



[5]Shi T(时涛), Zhao T-C(赵廷昌), Li G-Q(李国庆), Sun F-Z(孙福在). Polymorphism of LescPtoF gene sequences of tomato cultivated varieties in China. Plant Prot (植物保护), 2010, 36(3): 66–69 (in Chinese with English abstract)



[6]Bhullar N K, Mackay M, Keller B. Genetic diversity of the pm3 powdery mildew resistance alleles in wheat gene bank accessions as assessed by molecular markers. Diversity, 2010, 2(5): 768–786



[7]Zhang B(张彬), Zhang H-Y(张怀渝), Liu H-M(刘汉梅), Luo P-G(罗培高), Ren Z-L(任正隆). Diversity analysis of resistance gene analogs of wheat genetic varieties in Sichuan Province. J Triticeae Crops (麦类作物学报), 2006, 26(2): 21–26 (in Chinese with English abstract)



[8]Zhang J (张晋), Luo Z-Y(罗志勇), Yan Q(颜群), Zhong B(钟波), Gao L-J(高利军), Pan X-L(潘筱凌), Lai H-Y(赖海燕), Huang L(黄鹂), Gao H-L(高汉亮), Guo X-Q(郭小强). Genetic diversity amongst 45 main rice cultivars in Guangxi. J Southern Agric (南方农业学报), 2011, 42(10): 1173–1176 (in Chinese with English abstract)



[9]Hermann S, Brumbley S, McIntyre C L. Analysing diversity in sugarcane resistance gene analogues. Aust Plant Pathol, 2006, 35: 631–641



[10]Gan L(甘莉), Meng J-L(孟金陵). Genetic diversity analysis of brassica genus for the loci homologous to the disease resistant genes. Acta Agron Sin (作物学报), 2000, 26 (6): 650–658 (in Chinese with English abstract)



[11]Ji G-H(姬广海), Zhang S-G(张世光), Wei L-F(魏兰芳), Cui R-Q(崔汝强), Xu S-Z(徐绍忠). Preliminary analysis of resistance gene analogs for rice cultivars to bacterial blight in Yunnan. Acta Agron Sin (作物学报), 2004, 10(10): 969–974 (in Chinese with English abstract)



[12]Li C-L(李春来), Zhang H-Y(张怀渝). Research advance of resistance gene analogs in plant. Mol Plant Breed (分子植物育种), 2004, 2(6): 853–860 (in Chinese with English abstract)



[13]Ding G-H(丁国华), Chi C-Y(池眷玉), Wang G-L(王桂玲), Qin Z-W(秦智伟). Recent advances of studies on resistance gene analogs in plants. Chin Agric Sci Bull (中国农学通报), 2006, 1(22): 38–41 (in Chinese with English abstract)



[14]Chen Y M, Fan C M, Yang Y, He Y Q. Relationship between resistance gene analogue and blast resistance in rice. Rice Sci, 2009, 16(2): 99–105



[15]Ren J-S(任鄄胜), Xiao P-C(肖培村), Chen Y(陈勇), Huang X(黄湘), Wang Y-P(王玉平), Li S-G(李仕贵). Analysis on blast resistance phenotypes and resistance gene analog polymorphism of rice varieties. Sci Agric Sin (中国农业科学), 2009, 42(1): 1–9 (in Chinese with English abstract)



[16]Liu E-M(刘二明), Xiao Y-L(肖一龙), Yi Y-J(易有金), Zhuang J-Y(庄杰云), Zheng K-L(郑康乐), Luo F(罗峰). Relationship between phenotype of blast resistance and similarity of resistance gene analog in rice varieties. Chin J Rice Sci (中国水稻科学), 2005, 19(3): 209–216 (in Chinese with English abstract)



[17]Sun Y, Wang Y Y, He Y Q, Fan J H, Chen J B, Zhu Y Y. Analysis of the resistance gene analogue for rice cultivars in Yunnan Province. Sci Agric Sin, 2002, 35: 502–507



[18]Dellaporta S L, Wood J, Hicks J B. A plant DNA mini preparation: Version II. Plant Mol Biol, 1983, 1: 19–21



[19]Zhu Y Y, Sun Y, Wang Y Y, Li Y, He Y Q. Genetic analysis of rice varietal diversity for rice blast control. Acta Genet Sin, 2004, 31: 707–716



[20]Ma H-G(马辉刚), Huang R-R(黄瑞荣), Sun Y(孙雁), Zhu Y-Y(朱有勇), Liu K-C(刘康成). Genetic analysis of diversity of rice resistance to Magnaporthe grisea and its use for rice blast control. Acta Phytophy Sin (植物保护学报), 2006, 33(2): 113–116 (in Chinese with English abstract)



[21]Jiang Y-X(蒋有绪), trans. General Ecology Laboratory Manual (普通生态学实验室手册). Beijing: Science Press, 1979. pp 120–124 (in Chinese)



[22]Nei M. Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci USA, 1973, 70: 3321–3323



[23]Liu C-G(刘传光), Zhang G-Q(张桂权). SSR analysis of genetic diversity and the temporal trends of major commercial inbred indica rice cultivars in South China in 1949–2005. Acta Agron Sin (作物学报), 2010, 36(11): 1843–1852 (in Chinese with English abstract)



[24]Commission of Annals of Meizhou City (梅州市地方志编纂委员会). Annals of Meizhou City (梅州市志). Guangzhou: Guangzhou People's Publisher, 1999. pp 769-775 (in Chinese)



[25]de Vallavieille-Pope C. Management of disease resistance diversity of cultivars of a species in single fields: controlling epidemics. C R Biol, 2004, 327: 611–620



[26]Villaréal L M M A, Lannou C. Selection for increased spore efficacy by host genetic background in a wheat powdery mildew population. Phytopathology, 2000, 90: 1300–1306



[27]Gao C-X(高呈祥), Shang S-J(商世吉), Yang L-Q(杨立群), He Z-J(何传据), Zu X-Q(祖向前), Jiao W-H(焦伟华), Li S-F(李思芳). Rice varieties change in Heilongjiang Province and the relation between blast resistance and the change. Heilongjiang Agric Sci (黑龙江农业科学), 1989, (1): 31–34 (in Chinese with English abstract)



[28]Zeng L-X(曾列先), Chen S(陈深), Liu J-M(刘景梅), Si-Tu Z-M(司徒志谋), Chen S(陈森), Hu X-Y(胡学应), Luo S-H(罗森辉), Yang J-Y(杨健源), Lin P-Z(林佩珍), Zhu X-Y(朱小源). Evaluation of rice varieties resistance to bacterial blight in Guangdong. Guangdong Agric Sci (广东农业科学), 2006, (5): 38–40 (in Chinese with English abstract)



[29]Zhu X-Y(朱小源), Yang J-Y(杨健源), Liu J-M(刘景梅), Si-Tu Z-M(司徒志谋), Kang J-P(康金平), Hu X-Y(胡学应), Zhu M-J(朱敏记), Luo S-H(罗森辉), Yang Q-Y(杨祁云), Lin P-Z(林佩珍), Zeng L-X(曾列先), Jiang X-Y(姜先芽), Chen S(陈深). Evaluation on resistance of rice varieties in Guangdong to rice blast and strategy for its utilization. Guangdong Agric Sci (广东农业科学), 2006, (5): 34–37 (in Chinese with English abstract)



[30]Zhang X-M(张雪梅). Resistance evaluation of rice cultivar from Sichuan Province and genetic analysis of neihui99 to blast disease. MS. dissertation of Sichuan Agricultural University, 2011 (in Chinese with English abstract)



[31]Sha X-Y(沙学延), Zhu L-H(朱立宏). Pedigree of resistant rice cultivars to bacterial blight in China. J Nanjing Agric Univ (南京农业大学学报), 1993, 16(4): 6-12 (in Chinese with English abstract)



[32]Yang S-C(杨世诚), Sun M-L(孙茂林), Bi Y-Q(毕云青), Ran Y(冉云), Dai Y(戴云). Preliminary analysis on control of stripe rust epidemic using wheat variety groups with multi-resistant source. Yunnan Agric Sci Tech (云南农业科技), 1984, (6): 10–14 (in Chinese)



[33]Koizumi S. Rice blast control with multilines in Japan. In: Mew T W, Borrmeo E, Hardy B, eds. Exploiting Biodiversity for Sustainable Pest Management. Manila, Philippines: IRRI, 2001. pp 143–158



[34]Mundt C C. Use of multiline cultivars and cultivar mixtures for disease management. Annu Rev Phytopathol, 2002, 40: 381–410



[35]Zhu Y Y, Chen H R, Fan J H, Wang Y Y, Li Y, Chen J B, Fan J X, Yang S S, Hu L P, Leung H, Mew T W, Teng P S, Wang Z H, Mundt C C. Genetic diversity and disease control in rice. Nature, 2000, 406: 718–722
[1] 李增强, 丁鑫超, 卢海, 胡亚丽, 岳娇, 黄震, 莫良玉, 陈立, 陈涛, 陈鹏. 铅胁迫下红麻生理特性及DNA甲基化分析[J]. 作物学报, 2021, 47(6): 1031-1042.
[2] 卢海, 李增强, 唐美琼, 罗登杰, 曹珊, 岳娇, 胡亚丽, 黄震, 陈涛, 陈鹏. 红麻DNA甲基化响应镉胁迫及甲基化差异基因的表达分析[J]. 作物学报, 2021, 47(12): 2324-2334.
[3] 彭勃,赵晓雷,王奕,袁文娅,李春辉,李永祥,张登峰,石云素,宋燕春,王天宇,黎裕. 玉米叶向值的全基因组关联分析[J]. 作物学报, 2020, 46(6): 819-831.
[4] 王瑞莉,王刘艳,叶桑,郜欢欢,雷维,吴家怡,袁芳,孟丽姣,唐章林,李加纳,周清元,崔翠. 铝毒胁迫下甘蓝型油菜种子萌发期相关性状的QTL定位[J]. 作物学报, 2020, 46(6): 832-843.
[5] 王恒波,祁舒婷,陈姝琦,郭晋隆,阙友雄. 甘蔗栽培种单倍体基因组SSR位点的发掘与应用[J]. 作物学报, 2020, 46(4): 631-642.
[6] 张宏娟,李玉莹,苗丽丽,王景一,李超男,杨德龙,毛新国,景蕊莲. 小麦转录因子基因TaNAC67参与调控穗长和每穗小穗数[J]. 作物学报, 2019, 45(11): 1615-1627.
[7] 王恒波,肖乃衍,朱专为,刘翠翠,IntikhabALAM,陈平华,卢运海. 基于甘蔗热带种(LA-purple)全基因组序列的SSR开发及特征分析[J]. 作物学报, 2018, 44(9): 1400-1410.
[8] 董婧,逯晓萍,张坤明,薛春雷,张瑞霞. 高丹草杂种及其亲本转录组SNP及等位基因特异性表达分析[J]. 作物学报, 2018, 44(12): 1809-1817.
[9] 吴律,代力强,董青松,施婷婷,王丕武*. 玉米行粒数的全基因组关联分析[J]. 作物学报, 2017, 43(10): 1559-1564.
[10] 侯林涛,王腾岳,荐红举,王嘉,李加纳,刘列钊. 甘蓝型油菜盐胁迫下幼苗鲜重和干重QTL定位及候选基因分析[J]. 作物学报, 2017, 43(02): 179-189.
[11] 岳爱琴，李昂，毛新国，昌小平，柳玉平，李润植，景蕊莲. 小麦果聚糖合成酶基因6-SFT-D多态性及其与6-SFT-A2的累加效应[J]. 作物学报, 2016, 42(01): 11-18.
[12] 姚启伦,陈发波,刘红芳,方平. 基于Glb 1基因序列的西南地区玉米地方品种的系统进化[J]. 作物学报, 2015, 41(07): 998-1006.
[13] 周艳华,曹红利,岳川,王璐,郝心愿,王新超*,杨亚军*. 冷驯化不同阶段茶树DNA甲基化模式的变化[J]. 作物学报, 2015, 41(07): 1047-1055.
[14] 王嘉,荆凌云,荐红举,曲存民,谌利,李加纳,刘列钊. 甘蓝型油菜株高、第一分枝高和分枝数的QTL检测及候选基因筛选[J]. 作物学报, 2015, 41(07): 1027-1038.
[15] 吴林坤,黄伟民,王娟英,吴红淼,陈军,秦贤金,张重义,林文雄. 不同连作年限野生地黄根际土壤微生物群落多样性分析[J]. 作物学报, 2015, 41(02): 308-317.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2