欢迎访问作物学报,今天是

作物学报 ›› 2014, Vol. 40 ›› Issue (05): 942-949.doi: 10.3724/SP.J.1006.2014.00942

• 研究简报 • 上一篇    

花后高温胁迫下氮肥追施后移对小麦产量及旗叶生理特性的影响

江文文,尹燕枰*,王振林*,李勇,杨卫兵,彭佃亮,杨东清,崔正勇,卢昆丽,李艳霞   

  1. 山东农业大学农学院 / 作物生物学国家重点实验室,山东泰安271018
  • 收稿日期:2013-10-16 修回日期:2014-01-12 出版日期:2014-05-12 网络出版日期:2014-02-17
  • 通讯作者: 尹燕枰, E-mail: ypyin@sdau.edu.cn, Tel: 0538-8242458; 王振林, E-mail: zlwang@sdau.edu.cn, Tel: 0538-8241359
  • 基金资助:

    本研究由国家公益性行业(农业)科研专项经费项目(201203100, 201203029), 国家粮食丰产科技工程(2012BAD04B05), 山东省现代农业技术体系项目,国家自然科学基金项目(31271661)和国家重点基础研究发展计划(973计划)项目(2009CB118602)资助。

Effects of Postponed Application of Nitrogen Fertilizer on Yield and Physiological Characteristics of Flag Leaf in Wheat under Post-Anthesis Heat Stress

JIANG Wen-Wen,YIN Yan-Ping*,WANG Zhen-Lin*,LI Yong,YANG Wei-Bing,PENG Dian-Liang,YANG Dong-Qing,CUI Zheng-Yong,LU Kun-Li,LI Yan-Xia   

  1. Agronomy College, Shandong Agricultural University / State Key Laboratory of Crop Biology, Tai’an 271018, China?
  • Received:2013-10-16 Revised:2014-01-12 Published:2014-05-12 Published online:2014-02-17
  • Contact: 尹燕枰, E-mail: ypyin@sdau.edu.cn, Tel: 0538-8242458; 王振林, E-mail: zlwang@sdau.edu.cn, Tel: 0538-8241359

摘要:

为探明施用氮肥与减缓小麦花后高温伤害的关系并阐明其生理机制,于2011—2012年和2012—2013年连续两个生长季,选用小麦品种山农16和济麦22,在增温棚中进行花后11~15 d的高温胁迫处理,比较了氮肥全作基肥(T0)1/2基肥+1/2拔节期追肥(T1)1/2基肥+1/2孕穗期追肥(T2) 3种施氮方案下的产量、产量构成因素、旗叶光合特性及部分酶的活性。在高温胁迫下,T2方案较T0T1显著增加籽粒千粒重和产量,提高旗叶谷氨酰胺合成酶(GS)活性,增加旗叶气孔导度和光合速率,提高旗叶过氧化氢酶(CAT)和过氧化物酶(POD)活性,促进花前营养器官干物质向籽粒转运,增加开花后积累的干物质对籽粒的贡献率。两个品种结果基本一致。综合两年结果可以看出,T2处理显著优于T0T1处理,T2处理能显著缓解高温胁迫的伤害,提高籽粒产量。

关键词: 小麦, 旗叶, 高温胁迫, 氮肥追施后移, 籽粒产量

Abstract:

This study aimed to disclose the effect and physiological basis of postponed application of nitrogen fertilizer on alleviating the high-temperature damage after anthesis. In a two-year experiment from October 2011 to June 2013, winter wheat (Triticum aestivum L.) cultivars Shannong 16 and Jimai 22 were planted with three nitrogen treatments, namely T0 (total as basal fertilizer), T1 (a half of basal fertilizer and a half of nitrogen applied at jointing stage), and T2 (a half of basal fertilizer and a half applied at booting stage). High-temperature stress was simulated in plastic shed from 11 to 15 days after anthesis. Grain yield and its components, photosynthetic parameters of flag leaf, and activities of some important enzymes were compared among nitrogen treatments. The results indicated that T2 had higher 1000-grain weight and grain yield than T0 and T1 under heat stress. Besides, T2 had the effects on increasing glutamine synthetase (GS) activity, stomatal conductance, photosynthetic rate, and activities of hydrogen peroxidase (CAT) and peroxidase (POD) in flag leaf, stimulating translocation of pre-anthesis assimilate to grains, and increasing the contribution to grain of post-anthesis assimilate. Both cultivars had similar results. In an overall view, T2 is superior to T0 and T1 due to its significant effects on alleviating heat stress damage and increasing grain yield.

Key words: Wheat, Flag leaf, Heat stress, Postponed application of nitrogen fertilizer, Grain yield

[1]徐如强, 孙其信, 张树榛. 小麦耐热性研究现状与展望. 中国农业大学学报, 1998, 3(3): 33–40



Xu R Q, Sun Q X Zhang S Z. Current status and prospective on the investigation of heat tolerance in wheat. J China Agric Univ, 1998, 3(3): 33–40 (in Chinese with English abstract)



[2]Alexander L X, Zhang X, Peterson T C, Caesar J, Gleason B, Klein Tank A M G, Haylock M, Collins D, Trewin B, Rahimzadeh F, Tagipour A, Rupa Kumar K, Revadekar J, Griffiths G,, Vincent L, Stephenson D B, Burn J, Aguilar E, Brunet M, Taylor M, New M, Zhai P, Rusticucci M, Vazquez-Aguirre J L. Global observed changes in daily climate extremes of temperature and precipitation. J Geophys Res Atmospheres, 2006, 111: 1–22



[3]Senthold Asseng, Ian Foster, Nfil C, Turner. The impact of temperature variability on wheat yields. Global Change Biol, 2011, 17: 997-1012



[4]Wardlaw I F. Factors limiting the rate of dry matter accumulation in the grain of wheat grown at high temperature. Aust J Plant Physiol, 1980, 7: 387–400



[5]李永庚, 于振文, 张秀杰, 高雷明. 小麦产量与品质对灌浆不同阶段高温胁迫的响应. 植物生态学报, 2005, 29: 461–466



Li Y G, Yu Z W, Zhang X J, Gao L M. Response of yield and quality of wheat to heat stress at different grain filling stages. Chin J Plant Ecol, 2005, 29: 461–466 (in Chinese with English abstract)



[6]岳寿松, 于振文, 余松烈, 许玉敏. 不同生育时期施氮对冬小麦旗叶衰老和粒重的影响. 中国农业科学, 1997, 30(2): 42–46



Yue S S, Yu Z W, Yu S L, Xu Y M. Effects of nitrogen application at different growth stages on the senescence of flag leaves and grain yield in winter wheat (Triticum aestivum L.). Sci Agric Sin, 1997, 30(2): 42–46 (in Chinese with English abstract)



[7]Smith C J, White D M. Nitrogen accumulation and redistribution of late applied of N15 labeled fertilizer by wheat. Field Crops Res, 1990, 24: 221–228



[8]Pearmen I, Thomas S M. Thorne G N. Effects of nitrogen fertilizer on photosynthesis of several varieties of winter wheat. Ann Bot, 1997, 43: 613–621



[9]刘永环, 贺明荣, 王晓英, 张洪华. 不同氮肥基追比例对高温胁迫下小麦籽粒产量和品质的影响. 生态学报, 2009, 29: 5930–5935



Liu Y H, He M R, Wang X Y, Zhang H H. The effects of topdressing to basal nitrogen ratios on grain yield and quality of winter wheat under heat stresses during grain filling. Acta Ecol Sin, 2009, 29: 5930–5935 (in Chinese with English abstract)



[10]曹云英. 高温对水稻产量与品质的影响及其生理机制. 扬州大学博士学位论文, 2009



Cao Y Y. Effect of High Temperature on the Quality and Quantity of Rice Yield and Its Physiological Mechanism. PhD Dissertation of Yangzhou University, Yangzhou, China, 2009 (in Chinese with English abstract)



[11]徐晓玲, 王志敏, 张俊平. 灌浆期热胁迫对不同绿色器官光合性能的影响. 植物学报, 2001, 43: 571–577



Xu X L, Wang Z M, Zhang J P. Effect of heat stress on photosynthetic characteristics of different green organs of winter wheat during grain-filling stage. Acta Bot Sin, 2001, 43: 571–577 (in Chinese with English abstract)



[12]Perez-Soba M, Stulen L, L J M van der Eerden. Effect of atmospheric ammonia on the nitrogen metabolism of Scots pine (Pinus sylvestris) needles. Physiol Plant, 1994, 90: 629–636



[13]Silveira J A G, Matos J C S, Cecatto V M, Viegas R A, Oliveira J T A. Nitrate reductase activity, distribution, and response to nitrate in two contrasting Phaseolus species inoculated with Rhizobium spp. Environ Exp Bot, 2001, 46: 37–46



[14]Tan W, Liu J, Dai T, Jing Q, Cao W, Jiang D. Alterations in photosynthesis and antioxidant enzyme activity in winter wheat subjected to post-anthesis water-logging. Photosynthethetica, 2008, 46: 21–27



[15]Klapheck S, Zimmer I, Cosse H. Scavenging of hydrogen peroxide in the endosperm of Ricinus communis by ascorbate peroxidase. Plant Cell Physiol, 1990, 31: 1005–1013



[16]Blum A. Plant Breeding for Stress Environments. Boca Raton: CRC Press, 1996



[17]Wang G Y, Liu J M, Zhang Y, Yu B S, Shen Z Y. Studies on ultrstructure in common bean leaves during heat acclimation and heat stress. J Agric Biotech, 1999, 7: 151–156



[18]Liu X, Huang B. Heat stress injury in relation to membrane lipid peroxidation in creeping bentgrass. Crop Sci, 2000, 40: 503–513



[19]Xu S, Li J L, Zhang X Q, Wei H, Cui L G. Effects of heat acclimation pretreatment on changes of membrane lipid peroxidation, antioxidant metabolites, and ultrastructure of chloroplasts in two cool-season turfgrass species under heat stress. Environ Exp Bot, 2006, 56: 274–285



[20]Wang X, Cai J, Jiang D, Liu F L, Dai T B, Cao W X. Pre-anthesis high-temperature acclimation alleviates damage to the flag leaf caused by post-anthesis heat stress in wheat. J Plant Physiol, 2011, 168: 585–593



[21]段骅, 俞正华, 徐云姬, 王志琴, 刘立军, 杨建昌. 灌溉方式对减轻水稻高温危害的作用. 作物学报, 2012, 38: 107–120



Duan H, Yu Z H, Xu Y J, Wang Z Q, Liu L J, Yang J C. Role of irrigation patterns in reducing harms of high temperature to rice. ActaAgron Sin, 2012, 38: 107–120 (in Chinese with English abstract)



[22]于振文, 田奇卓, 潘庆民, 岳寿松, 王东, 段藏禄, 段玲玲, 王志军, 牛运生. 黄淮麦区冬小麦超高产栽培的理论与实践. 作物学报, 2002, 28: 577–585



Yu Z W, Tian Q Z, Pan Q M, Yue S S, Wang D, Duan Z L, Duan L L, Wang Z J, Niu Y S. Theory and practice on cultivation of super high yield of winter wheat in the wheat fields of Yellow River and Huaihe River district. Acta Agron Sin, 2002, 28: 577–585 (in Chinese with English abstract)



[23]潘庆民, 于振文. 追氮时期对冬小麦籽粒品质和产量的影响. 麦类作物学报, 2002, 22(2): 65–69



Pan Q M, Yu Z W. Effects of nitrogen topdressing stage on grain quality and yield of winter wheat. J Triticeae Crops, 2002, 22(2): 65–69 (in Chinese with English abstract)



[24]王月福, 姜东, 于振文, 曹卫星. 高低土壤肥力下小麦基施和追施氮肥的利用效率和增产效应, 作物学报, 2003, 29: 491–495



Wang Y F, Jiang D, Yu Z W, Cao W X. Nitrogen use efficiency and yield of wheat with basal and top-dressed nitrogen fertilizers in soils with different fertility. Acta Agron Sin, 2003, 29: 491–495 (in Chinese with English abstract)



[25]季书勤, 赵淑章, 吕凤荣, 刘媛媛, 王绍中. 小麦前氮后移的施肥效应与有效使用条件. 作物学报, 1998, 24: 889–893



Ji S Q, Zhao S Z, Lü F R, Liu Y Y, Wang S Z. The effect of nitrogen application under different conditions at late growth stage of wheat. Acta Agron Sin, 1998, 24: 889–893 (in Chinese with English abstract)



[26]Ruiz J M, Rivero R M, Garcia P C, Baghour M, Romero L. Role of CaCl2 in nitrate assimilation in leaves and roots of tobacco plants (Nicotiana tabacum L.). Plant Sci, 1999, 141: 107–115



[27]Solomonson L P, Spehar A M. Model for the regulation of nitrate assimilation. Nature, 1977, 265: 373–375



[28]Ben J M, Dimah Z H. The role of glutamine synthetase and glutamate dehydrogenase in nitrogen assimilation and possibilities for improvement in the nitrogen utilization of crops. J Exp Bot, 2002, 53: 979–987



[29]Noctor G, Fover C H. Ascorbate and glutathione: keeping active oxygen under control. Annu Rev Plant Mol Biol, 1998, 49: 249–279



[30]Blum A, Ebercon A. Cell membrane stability as a measure of drought and heat tolerance in wheat. Crop Sci, 1981, 21: 43–47



[31]张生杰, 黄元炯, 任庆成, 张小全, 杨志晓, 杨铁钊. 氮素对不同品种烤烟叶片衰老、光合特性及产量和品质的影响. 应用生态学报, 2010, 21: 668–674



Zhang S J, Huang Y J, Ren Q C, Zhang X Q, Yang Z X, Yang T Z. Effects of nitrogen fertilization on leaf senescence, photosynthetic characteristics, yield, and quality of different flue-cured tobacco varieties. Chin J Appl Ecol, 2010, 21: 668–674 (in Chinese with English abstract)



[32]Evans L T, Dunstone R L. Some physiological aspects of evolution in wheat. Aust J Biol Sci, 1970, 23: 725–741



[33]韩占江, 于振文, 王东, 张永丽. 测墒补灌对冬小麦干物质积累与分配及水分利用效率的影响. 作物学报, 2010, 36: 457–465



Han Z J, Yu Z W, Wang D, Zhang Y L. Effects of supplemental irrigation based on testing soil moisture on dry matter accumulation and distribution and water use efficiency in winter wheat. Acta Agron Sin, 2010, 36: 457-465 (in Chinese with English abstract)

[1] 胡文静, 李东升, 裔新, 张春梅, 张勇. 小麦穗部性状和株高的QTL定位及育种标记开发和验证[J]. 作物学报, 2022, 48(6): 1346-1356.
[2] 郭星宇, 刘朋召, 王瑞, 王小利, 李军. 旱地冬小麦产量、氮肥利用率及土壤氮素平衡对降水年型与施氮量的响应[J]. 作物学报, 2022, 48(5): 1262-1272.
[3] 付美玉, 熊宏春, 周春云, 郭会君, 谢永盾, 赵林姝, 古佳玉, 赵世荣, 丁玉萍, 徐延浩, 刘录祥. 小麦矮秆突变体je0098的遗传分析与其矮秆基因定位[J]. 作物学报, 2022, 48(3): 580-589.
[4] 冯健超, 许倍铭, 江薛丽, 胡海洲, 马英, 王晨阳, 王永华, 马冬云. 小麦籽粒不同层次酚类物质与抗氧化活性差异及氮肥调控效应[J]. 作物学报, 2022, 48(3): 704-715.
[5] 刘运景, 郑飞娜, 张秀, 初金鹏, 于海涛, 代兴龙, 贺明荣. 宽幅播种对强筋小麦籽粒产量、品质和氮素吸收利用的影响[J]. 作物学报, 2022, 48(3): 716-725.
[6] 马红勃, 刘东涛, 冯国华, 王静, 朱雪成, 张会云, 刘静, 刘立伟, 易媛. 黄淮麦区Fhb1基因的育种应用[J]. 作物学报, 2022, 48(3): 747-758.
[7] 王洋洋, 贺利, 任德超, 段剑钊, 胡新, 刘万代, 郭天财, 王永华, 冯伟. 基于主成分-聚类分析的不同水分冬小麦晚霜冻害评价[J]. 作物学报, 2022, 48(2): 448-462.
[8] 陈新宜, 宋宇航, 张孟寒, 李小艳, 李华, 汪月霞, 齐学礼. 干旱对不同品种小麦幼苗的生理生化胁迫以及外源5-氨基乙酰丙酸的缓解作用[J]. 作物学报, 2022, 48(2): 478-487.
[9] 徐龙龙, 殷文, 胡发龙, 范虹, 樊志龙, 赵财, 于爱忠, 柴强. 水氮减量对地膜玉米免耕轮作小麦主要光合生理参数的影响[J]. 作物学报, 2022, 48(2): 437-447.
[10] 马博闻, 李庆, 蔡剑, 周琴, 黄梅, 戴廷波, 王笑, 姜东. 花前渍水锻炼调控花后小麦耐渍性的生理机制研究[J]. 作物学报, 2022, 48(1): 151-164.
[11] 孟颖, 邢蕾蕾, 曹晓红, 郭光艳, 柴建芳, 秘彩莉. 小麦Ta4CL1基因的克隆及其在促进转基因拟南芥生长和木质素沉积中的功能[J]. 作物学报, 2022, 48(1): 63-75.
[12] 韦一昊, 于美琴, 张晓娇, 王露露, 张志勇, 马新明, 李会强, 王小纯. 小麦谷氨酰胺合成酶基因可变剪接分析[J]. 作物学报, 2022, 48(1): 40-47.
[13] 李玲红, 张哲, 陈永明, 尤明山, 倪中福, 邢界文. 普通小麦颖壳蜡质缺失突变体glossy1的转录组分析[J]. 作物学报, 2022, 48(1): 48-62.
[14] 赵文青, 徐文正, 杨锍琰, 刘玉, 周治国, 王友华. 棉花叶片响应高温的差异与夜间淀粉降解密切相关[J]. 作物学报, 2021, 47(9): 1680-1689.
[15] 罗江陶, 郑建敏, 蒲宗君, 范超兰, 刘登才, 郝明. 四倍体小麦与六倍体小麦杂种的染色体遗传特性[J]. 作物学报, 2021, 47(8): 1427-1436.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!