作物学报 ›› 2014, Vol. 40 ›› Issue (08): 1350-1355.doi: 10.3724/SP.J.1006.2014.01350
王丽1,2,张明才1,杜明伟1,田晓莉1,李召虎1
WANG Li1,2,ZHANG Ming-Cai1,DU Ming-Wei1,TIAN Xiao-Li1,LI Zhao-Hu1,*
摘要:
室内盆栽欣抗4,在棉花幼苗第3片真叶完全展平时(第4叶未展开)叶面喷施甲哌鎓(DPC),研究DPC对棉花幼苗叶片生长的控制与赤霉素(GA)合成早期关键酶柯巴基焦磷酸合酶(CPS)和内根-贝壳杉烯合酶(KS)基因表达的关系。结果表明,DPC处理显著减小棉花幼苗第3和第4叶的叶面积,第4叶叶面积受控制程度较第3叶大;80 mg L–1DPC处理的棉花幼苗第3和4叶中GA4含量分别于处理后4 d和4~6 d显著低于对照;与对照相比,80 mg L–1 DPC处理的棉花幼苗第3叶中GhCPS和GhKS表达在处理后1~4 d显著降低,而第4叶中GhCPS和GhKS的表达在处理后1~6 d显著降低。由此可见,DPC通过影响GhCPS和GhKS的表达,降低内源活性GA4的含量,控制棉花幼苗叶片生长,且较幼嫩叶片对DPC较敏感。
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