作物学报 ›› 2014, Vol. 40 ›› Issue (08): 1350-1355.doi: 10.3724/SP.J.1006.2014.01350
王丽1,2,张明才1,杜明伟1,田晓莉1,李召虎1
WANG Li1,2,ZHANG Ming-Cai1,DU Ming-Wei1,TIAN Xiao-Li1,LI Zhao-Hu1,*
摘要:
室内盆栽欣抗4,在棉花幼苗第3片真叶完全展平时(第4叶未展开)叶面喷施甲哌鎓(DPC),研究DPC对棉花幼苗叶片生长的控制与赤霉素(GA)合成早期关键酶柯巴基焦磷酸合酶(CPS)和内根-贝壳杉烯合酶(KS)基因表达的关系。结果表明,DPC处理显著减小棉花幼苗第3和第4叶的叶面积,第4叶叶面积受控制程度较第3叶大;80 mg L–1DPC处理的棉花幼苗第3和4叶中GA4含量分别于处理后4 d和4~6 d显著低于对照;与对照相比,80 mg L–1 DPC处理的棉花幼苗第3叶中GhCPS和GhKS表达在处理后1~4 d显著降低,而第4叶中GhCPS和GhKS的表达在处理后1~6 d显著降低。由此可见,DPC通过影响GhCPS和GhKS的表达,降低内源活性GA4的含量,控制棉花幼苗叶片生长,且较幼嫩叶片对DPC较敏感。
[1]Siebert J D, Stewart A M. Influence of plant density on cotton response to mepiquat chloride application. Agron J, 2006, 98: 1634–1639[2]Ren X, Zhang L, Du M, Eversc J B, Werf W, Tian X, Li Z. Managing mepiquat chloride and plant density for optimal yield and quality of cotton. Field Crops Res, 2013, 149: 1–10[3]Reddy V R, Baker D N, Hodges H F. Temperature and mepiquat chloride effects on cotton canopy architecture. Agron J, 1990, 82: 190–195[4]Reddy A R, Reddy K R, Hodges H F. Mepiquat chloride (PIX) induced changes in photosynthesis and growth of cotton. Plant Growth Regul, 1996, 20: 179–183[5]Zhao D, Oosterhuis D M. Pix plus and mepiquat chloride effects on physiology, growth, and yield of field-grown cotton. J Plant Growth Regul, 2000, 19: 415–422[6]Gonias E D, Oosterhuis D M, Bibi A C. Cotton radiation use efficiency response to plant growth regulators. J Agric Sci, 2012, 150: 595–602[7]Rademacher W. Growth retardants: effects on gibberellin biosynthesis and other metabolic pathways. Annu Rev Plant Physiol Mol Biol, 2000, 51: 501–531[8]Dennis D T, Upper C D, West C A. An enzymic site of inhibition of gibberellin biosynthesis by Amo 1618 and other plant growth retardants. Plant Physiol, 1965, 40: 948–952[9]Shechter I, West C A. Biosynthesis of Gibberellins. IV. Biosynthesis of cyclic diterpenes from trans-geranylgeranyl pyrophosphate. J Biol Chem, 1969, 244: 3200–3209[10]Smith M W, Yamaguchi S, Ait-Ali T, Kamiya Y. The first step of gibberellin biosynthesis in pumpkin is catalyzed by at least two copalyl diphosphate synthases encoded by differentially regulated genes. Plant Physiol, 1998, 118: 1411–1419[11]Silverstone A L, Chang C, Krol E, Sun T P. Developmental regulation of the gibberellin biosynthetic gene GA1 in Arabidopsis thaliana. Plant J, 1997, 12: 9–19[12]Koornnef M, van der Veen J H. Induction and analysis of gibberellin-sensitive mutants in Arabidopsis thaliana (L.) Heynh. Theor Appl Genet, 1980, 58:257–263[13]Sun T, Kamiya Y. The Arabidopsis GAl locus encodes the cyclase ent-kaurene synthetase A of gibberellin biosynthesis. Plant Cell, 1994, 6: 1509–1518[14]Reddy K R, Kakani V G, Zhao D, Mohammed A R, Gao W. Cotton responses to ultraviolet-B radiation: experimentation and algorithm development. Agr Forest Meteorol, 2003, 120: 249–265[15]何钟佩. 农作物化学控制实验指导. 北京: 北京农业大学出版社, 1993. pp 36–39He Z P. Experimental guide of chemical control of crops. Beijing: Beijing Agricultural University Press, 1993. pp 36–39 (in Chinese)[16]Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCt method. Methods, 2001, 25: 402–408[17]Barbosa L M, Castro P R C. Comparison between concentrations and application time of mepiquat chloride, chlorocholine chloride and ethephon in cotton (Gossypium hirsutum L. cv. IAC-17). Planta Daninha, 1983, 6: 1–10[18]Fernández C J, Cothren J T, McInnes K J. Partitioning of biomass in well-watered and water-stressed cotton plants treated with mepiquat chloride. Crop Sci, 1991, 31:1224–1228[19]陈吟, 张明才, 李召虎. 棉花和玉米对缩节安吸收、转运与分配的特征研究. 中国科技论文在线, http://www.paper.edu.cn/releasepaper/ content/201204-227 Chen Y, Zhang M, Li Z. The absorption and translocation of mepiquat chloride in maize (Zea mays L.) and cotton (Gossypium spp.). Chinese Science Paper, http://www.paper.edu.cn/releasepaper/content/201204-227 (in Chinese with English abstract)[20]Olszewski N, Sun T P, Gubler F. Gibberellin signaling: biosynthesis, catabolism, and response pathways. Plant Cell, 2002, 14: 61–80[21]Yamaguchi S. Gibberellin metabolism and its regulation. Annu Rev Plant Biol, 2008, 59: 225–251[22]Jiang X, Li H, Wang T, Peng C, Wang H, Wu H, Wang X. Gibberellin indirectly promotes chloroplast biogenesis as a means to maintain the chloroplast population of expanded cells. Plant J, 2012, 72: 768–780[23]Ross J J, Murfet I C, Reid J B. Gibberellin mutants. Physiol Plant, 1997, 100: 550–560[24]Kang S M, Kimb J T, Hamayun M, Hwang I C, Khan A L, Kim Y H, Lee J H, Lee I J. Influence of prohexadione-calcium on growth and gibberellins content of Chinese cabbage grown in alpine region of South Korea. Sci Hortic, 2010, 125: 88–92[25]Otani M, Meguro S, Gondaira H, Hayashi M, Saito M, Han D S, Inthima P, Supaibulwatana K, Mori S, Jikumaru Y, Kamiya Y, Li T, Niki T, Nishijima T, Koshioka M, Nakano M. Overexpression of the gibberellin 2-oxidase gene from Torenia fournieri induces dwarf phenotypes in the liliaceous monocotyledon Tricyrtis sp. J Plant Physiol, 2013, 170: 1416–1423[26]Yamaguchi S, Sun T P, Kawaide H, Kamiya Y. The GA2 locus of Arabidopsis thaliana encodes ent-kaurene synthase of gibberellin biosynthesis. Plant Physiol, 1998, 116: 1271–1278[27]Ayele B T, Ozga J A, Kurepin L V, Reinecke D M. Developmental and embryo axis regulation of gibberellin biosynthesis during germination and young seedling growth of pea. Plant Physiol, 2006, 142: 1267–1281[28]Yamaguchi S, Kamiya Y, Sun T P. Distinct cell-specific expression patterns of early and late gibberellin biosynthetic genes during Arabidopsis seed germination. Plant J, 2001, 28:443–453[29]李晨晨, 侯雷, 尹亮, 赵金凤, 袁守江, 张文会, 李学勇. 水稻极矮突变体s2-47对赤霉素的响应及基因定位研究. 作物学报, 2013, 39: 1766−1774Li C C, Hou L, Yin L, Zhao J F, Yuan S J, Zhang W H, Li X Y. Gibberellin responsiveness and gene mapping of rice extreme dwarf mutant s2-47. Acta Agron Sin, 2013, 39: 1766−1774 (in Chinese with English abstract) |
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