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作物学报 ›› 2014, Vol. 40 ›› Issue (10): 1877-1883.doi: 10.3724/SP.J.1006.2014.01877

• 研究简报 • 上一篇    

118份甘蔗种质资源遗传多样性的AFLP分析

昝逢刚,吴才文,陈学宽,赵培方,赵俊,刘家勇*   

  1. 云南省农业科学院甘蔗研究所 / 云南省甘蔗遗传改良重点试验室,云南开远661699
  • 收稿日期:2014-04-04 修回日期:2014-07-06 出版日期:2014-10-12 网络出版日期:2014-07-25
  • 基金资助:

    本研究由国家现代农业产业技术体系建设专项(CARS-20-1-1), 云南省应用基础研究面上项目(2010CD003)和云南省重点新产品开发计划项目(2012BB014)资助。

Genetic Diversity Analysis of 118 Sugarcane Germplasm with AFLP Markers

ZAN Feng-Gang,WU Cai-Wen,CHEN Xue-Kuan,ZHAO Pei-Fan,ZHAO Jun,LIU Jia-Yong*   

  1. Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences / Yunnan Province Key Laboratory of Sugarcane Genetic Improvement, Kuaiyuan 616699, China
  • Received:2014-04-04 Revised:2014-07-06 Published:2014-10-12 Published online:2014-07-25

摘要:

采用AFLP标记技术,对来自中国、澳大利亚、美国、菲律宾、巴西和法国的118份甘蔗种质进行遗传多样性分析。采用10对引物组合共扩增出1310条谱带,其中多态性条带为1195多态性条带比率为91.2%118种质间的相似性系数在0.583~0.929之间,平均为0.750,多态信息量为0.2332,每个位点的有效等位基因数为1.3789。结果显示在相似性系数0.69处切割时,可划分为2个类群,第I类群包括粤糖00-236、云蔗04-622SP80-1816;第II类群有115份种质,在相似性系数0.74处切割时,可将第II类群划分为5个亚群。各国甘蔗种质亲缘关系较近,其中美国种质遗传多样性相对较丰富。

关键词: 甘蔗, 种质, AFLP, 遗传多样性

Abstract:

AFLP markers were used to assess the genetic diversity of 118 sugarcane germplasm from China, Australia, USA, Philippines, Brazil, and France. A total of 1310 bands were amplified with ten pairs of primers, 1195 of which were polymorphic bands, the percentage of polymorphic loci was 91.22%. The genetic similarity coefficient among 118 sugarcane germplasm ranged from 0.583 to 0.929 with an average of 0.750, the polymorphism information content was 0.2332, and the effective number of alleles per loci was 1.3789. The results showed 118 germplasm were divided into two groups at 0.69 of the similarity coefficient. The group I consisted of YT00-236, YZ04-622 and SP80-1816, which shared lower genetic similarity with the rest of 115 germplasm in another group. The 115 germplasm were divided into five sub-groups at 0.74 of the similarity coefficient. The sugarcane germplasm shared high genetic similarity among countries, and there existed much higher genetic diversity within germplasm introduced from USA.

Key words: Sugarcane, Germplasm, AFLP, Genetic diversity

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