118份甘蔗种质资源遗传多样性的AFLP分析

doi:10.3724/SP.J.1006.2014.01877

摘要/Abstract

摘要：

采用AFLP标记技术，对来自中国、澳大利亚、美国、菲律宾、巴西和法国的118 份甘蔗种质进行遗传多样性分析。采用10对引物组合共扩增出1310条谱带，其中多态性条带为1195条，多态性条带比率为91.2%。118份种质间的相似性系数在0.583~0.929之间，平均为0.750，多态信息量为0.2332，每个位点的有效等位基因数为1.3789。结果显示在相似性系数0.69处切割时，可划分为2个类群，第I类群包括粤糖00-236、云蔗04-622和SP80-1816；第II类群有115份种质，在相似性系数0.74处切割时，可将第II类群划分为5个亚群。各国甘蔗种质亲缘关系较近，其中美国种质遗传多样性相对较丰富。

关键词: 甘蔗, 种质, AFLP, 遗传多样性

Abstract:

AFLP markers were used to assess the genetic diversity of 118 sugarcane germplasm from China, Australia, USA, Philippines, Brazil, and France. A total of 1310 bands were amplified with ten pairs of primers, 1195 of which were polymorphic bands, the percentage of polymorphic loci was 91.22%. The genetic similarity coefficient among 118 sugarcane germplasm ranged from 0.583 to 0.929 with an average of 0.750, the polymorphism information content was 0.2332, and the effective number of alleles per loci was 1.3789. The results showed 118 germplasm were divided into two groups at 0.69 of the similarity coefficient. The group I consisted of YT00-236, YZ04-622 and SP80-1816, which shared lower genetic similarity with the rest of 115 germplasm in another group. The 115 germplasm were divided into five sub-groups at 0.74 of the similarity coefficient. The sugarcane germplasm shared high genetic similarity among countries, and there existed much higher genetic diversity within germplasm introduced from USA.

Key words: Sugarcane, Germplasm, AFLP, Genetic diversity

昝逢刚，吴才文，陈学宽，赵培方，赵俊，刘家勇*. 118份甘蔗种质资源遗传多样性的AFLP分析[J]. 作物学报, 2014, 40(10): 1877-1883.

ZAN Feng-Gang,WU Cai-Wen,CHEN Xue-Kuan,ZHAO Pei-Fan,ZHAO Jun,LIU Jia-Yong*. Genetic Diversity Analysis of 118 Sugarcane Germplasm with AFLP Markers[J]. Acta Agron Sin, 2014, 40(10): 1877-1883.

参考文献

[1] 李奇伟, 陈子云, 梁洪.现代甘蔗改良技术. 广州: 华南理工大学出版社, 2000. pp 11–18

Li Q W, Chen Z Y, Liang H. Modern Technology for Sugarcane Improvement. Guangzhou: South China University of Technology Press, 2000. pp 11–18 (in Chinese)

[2] 邓海华, 李奇伟. CP72-1210在我国甘蔗育种中的利用. 广东农业科学, 2007, 11: 18–21

Deng H H, Li Q W. Utilization of CP72-1210 in sugarcane breeding program in mainland China. Guangdong J Agric Sci, 2007, 11: 18–21 (in Chinese with English abstract)

[3] AitkenK S, Li J C, Jackson P, Piperidis G, McIntyre C L. AFLP analysis of genetic diversity within Saccharum officinarum and comparison with sugarcane cultivars. Austr J Agric Res, 2006, 57: 1167–1184

[4] Selvi A, Nair N V, Noyer J L, Singh N K, Balasundaram N, Bansal K C, Koundal K R, Mohapatra T. AFLP analysis of the phenetic organization and genetic diversity in the sugarcane complex, Saccharum and Erianthus. Genet Resour Crop Evol, 2006, 53: 831–842

[5] Harvey M, Huckett B I, Botha F C. Use of Polymerase Chain Reaction (PCR), and Random Amplification of Polymorphic DNAs, RAPDs, for the Determination of Genetic Distances between 21 Sugarcane Varieties. In: Proceeding of South African Sugar Technologists Association, 1994, 68: 36–40

[6] Nair N V, Selvi A, Sreenivasan T V, Pushpalatha K N. Molecular diversity in Indian sugarcane cultivars as revealed by randomly amplified DNA polymorphisms. Euphytica, 2002, 127: 219–225

[7] Lima M L A, Garcia A A F, Oliveira K M, Matsuoka S, Arizono H, Souza C L J, Souza A P. Analysis of genetic similarity detected by AFLP and coefficient of parentage among genotypes of sugar cane (Saccharum spp.). Theor Appl Genet, 2002, 104: 30–38

[8] 庄南生, 郑成木, 黄东益, 唐燕琼, 高和琼. 甘蔗种质遗传基础的AFLP分析. 作物学报, 2005, 31: 444–450

Zhuang N S, Zheng C M, Huang D Y, Tang Y Q, Gao H Q. AFLP analysis for sugarcane germplasms. Acta Agron Sin, 2005, 31: 444–450 (in Chinese with English abstract)

[9] 刘家勇, 赵培方, 刘新龙, 赵俊, 杨昆, 吴才文, 应雄美, 昝逢刚, 陈学宽. 68份国外甘蔗种质资源遗传多样性的AFLP分析. 湖南农业大学学报, 2013, 39: 466–470

Liu J Y, Zhao P F, Liu X L, Zhao J, Yang K, Wu C W, Ying X M, Zan F G, Chen X K. Genetic diversity analysis on 68 foreign sugarcane germplasms (Saccharum spp.) with AFLP technique. J Hunan Agric Univ, 2013, 39: 466–470 (in Chinese with English abstract)

[10] Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M. AFLP: a new technique for DNA fingerprinting. Nucl Acids Res, 1995, 23: 4407–4414

[11] 劳方业, 刘睿, 何慧怡, 邓海华, 陈仲华, 陈健文, 符成, 张垂明, 杨业后. 崖城系列甘蔗亲本遗传多样性的AFLP标记分析. 分子植物育种, 2008, 6: 517–522

Lao F Y, Liu R, He H Y, Deng H H, Chen Z H, Chen J W, Fu C, Zhang C M, Yang Y H. AFLP analysis of genetic diversity in series sugarcane parents developed at HSBS. Mol Plant Breed, 2008, 6: 517–522 (in Chinese with English abstract)

[12] Schenck S, Crepeau M W, Wu K K, Moore P H, Yu Q, Ming R. Genetic diversity and relationships in native Hawaiian Saccharum officinarum sugarcane. J Hered, 2004, 95: 327–331

[13] 李鸣, 谭裕模, 李杨瑞, 李容柏, 高国庆. 甘蔗(Saccharum officinarum L.)品种遗传差异的AFLP分子标记分析. 作物学报, 2004, 30: 1008–1013

Li M, Tan Y M, Li Y R, Li R B, Gao G Q. AFLP molecular analysis of genetic difference between cultivars in sugarcane (Saccharum officinarum L). Acta Agron Sin, 2004, 30: 1008–1013 (in Chinese with English abstract)

[14] 刘新龙, 毛钧, 陆鑫, 马丽, Karen S A, Phillip A J, 蔡青, 范源洪. 甘蔗SSR和AFLP分子遗传连锁图谱构建. 作物学报, 2010, 36: 177–183

Liu X L, Mao J, Lu X, Ma L, Karen S A, Phillip A J, Cai Q, Fan Y H. Construction of molecular genetic linkage map of sugarcane based on SSR and AFLP markers. Acta Agron Sin, 2010, 36: 177–183 (in Chinese with English abstract)

[15] 吴转娣, 昝逢刚, 赵丽宏, 罗遵喜, 张树珍. 甘蔗基因组DNA小量提取与大量提取方法研究. 生物技术通报, 2009, (增刊-1): 172–175

Wu Z Z, Zan F G, Zhao L H, Luo Z X, Zhang S Z. Study on mini and mass-extraction methods of sugarcane genomic DNA. Biotechnol Bull, 2009, (suppl-1): 172–175 (in Chinese with English abstract)

[16] 刘新龙, 蔡青, 毕燕, 陆鑫, 马丽, 应雄美, 毛钧. 甘蔗AFLP标记和SSR标记的PAGE胶快速银染检测方法. 江苏农业学报, 2009, 25: 433–435

Liu X L, Cai Q, Bi Y, Lu X, Ma L, Ying X M, Mao J. A rapid silver staining method for PAGE used in sugarcane AFLP and SSR molecular markers. Jiangsu J Agric Sci, 2009, 25: 433–435 (in Chinese with English abstract)

[17] 劳方业, 刘睿, 何慧怡, 邓海华, 李奇伟, 陈仲华, 陈健文, 符成, 齐永文, 张垂明. 我国甘蔗亲本遗传多样性的AFLP标记分析. 基因组学与应用生物学, 2009, 28: 503–508

Lao F Y, Liu R, He H Y, Deng H H, Li Q W, Chen Z H, Chen J W, Fu C, Qi Y W, Zhang C M. Genetic diversity analysis of sugarcane parents with AFLP in China. Genom Appl Biol, 2009, 28: 503–508 (in Chinese with English abstract)

相关文章 15

[1]	肖颖妮, 于永涛, 谢利华, 祁喜涛, 李春艳, 文天祥, 李高科, 胡建广. 基于SNP标记揭示中国鲜食玉米品种的遗传多样性[J]. 作物学报, 2022, 48(6): 1301-1311.
[2]	张钰坤, 陆赢, 崔看, 夏石头, 刘忠松. 芥菜种子颜色调控基因TT8的等位变异及其地理分布分析[J]. 作物学报, 2022, 48(6): 1325-1332.
[3]	秦璐, 韩配配, 常海滨, 顾炽明, 黄威, 李银水, 廖祥生, 谢立华, 廖星. 甘蓝型油菜耐低氮种质筛选及绿肥应用潜力评价[J]. 作物学报, 2022, 48(6): 1488-1501.
[4]	肖健, 陈思宇, 孙妍, 杨尚东, 谭宏伟. 不同施肥水平下甘蔗植株根系内生细菌群落结构特征[J]. 作物学报, 2022, 48(5): 1222-1234.
[5]	周慧文, 丘立杭, 黄杏, 李强, 陈荣发, 范业赓, 罗含敏, 闫海锋, 翁梦苓, 周忠凤, 吴建明. 甘蔗赤霉素氧化酶基因ScGA20ox1的克隆及功能分析[J]. 作物学报, 2022, 48(4): 1017-1026.
[6]	孔垂豹, 庞孜钦, 张才芳, 刘强, 胡朝华, 肖以杰, 袁照年. 不同施肥水平下丛枝菌根真菌对甘蔗生长及养分相关基因共表达网络的影响[J]. 作物学报, 2022, 48(4): 860-872.
[7]	陈小红, 林元香, 王倩, 丁敏, 王海岗, 陈凌, 高志军, 王瑞云, 乔治军. 基于高基元SSR构建黍稷种质资源的分子身份证[J]. 作物学报, 2022, 48(4): 908-919.
[8]	杨宗桃, 刘淑娴, 程光远, 张海, 周营栓, 商贺阳, 黄国强, 徐景升. 甘蔗类泛素蛋白UBL5应答SCMV侵染及其与SCMV-6K2的互作[J]. 作物学报, 2022, 48(2): 332-341.
[9]	胡亮亮, 王素华, 王丽侠, 程须珍, 陈红霖. 绿豆种质资源苗期耐盐性鉴定及耐盐种质筛选[J]. 作物学报, 2022, 48(2): 367-379.
[10]	张鹤, 蒋春姬, 殷冬梅, 董佳乐, 任婧瑶, 赵新华, 钟超, 王晓光, 于海秋. 花生耐冷综合评价体系构建及耐冷种质筛选[J]. 作物学报, 2021, 47(9): 1753-1767.
[11]	张海, 程光远, 杨宗桃, 刘淑娴, 商贺阳, 黄国强, 徐景升. 甘蔗PsbR亚基应答SCMV侵染及其与SCMV-6K2的互作[J]. 作物学报, 2021, 47(8): 1522-1530.
[12]	傅华英, 张婷, 彭文静, 段瑶瑶, 许哲昕, 林艺华, 高三基. 甘蔗新品种(系)苗期白条病人工接种抗性鉴定与评价[J]. 作物学报, 2021, 47(8): 1531-1539.
[13]	王琰琰, 王俊, 刘国祥, 钟秋, 张华述, 骆铮珍, 陈志华, 戴培刚, 佟英, 李媛, 蒋勋, 张兴伟, 杨爱国. 基于SSR标记的雪茄烟种质资源指纹图谱库的构建及遗传多样性分析[J]. 作物学报, 2021, 47(7): 1259-1274.
[14]	苏亚春, 李聪娜, 苏炜华, 尤垂淮, 岑光莉, 张畅, 任永娟, 阙友雄. 甘蔗割手密种类甜蛋白家族鉴定及栽培种同源基因功能分析[J]. 作物学报, 2021, 47(7): 1275-1296.
[15]	黄宁, 惠乾龙, 方振名, 李姗姗, 凌辉, 阙友雄, 袁照年. 甘蔗β-胡萝卜素异构酶基因家族的鉴定、定位和表达分析[J]. 作物学报, 2021, 47(5): 882-893.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed