作物学报 ›› 2015, Vol. 41 ›› Issue (04): 585-592.doi: 10.3724/SP.J.1006.2015.00585
赵青平,赵翔,慕世超,肖慧丽,张骁*
ZHAO Qing-Ping,ZHAO Xiang,MU Shi-Chao,XIAO Hui-Li,ZHANG Xiao*
摘要:
向光素PHOT1介导较宽范围蓝光诱导的下胚轴向光弯曲,而向光素PHOT2仅在强蓝光下起作用。强蓝光下, PHOT1和PHOT2介导拟南芥下胚轴向光弯曲的功能冗余性,限制了人们对PHOT2功能的研究。为此,以拟南芥phot1突变体为材料,避开PHOT1基因的干扰,通过EMS诱变筛选拟南芥下胚轴向光不弯曲突变体, 成功克隆到1个基因,命名为P2SA2 (phototropin 2 signaling associated 2),该基因被证明是NPH3的等位基因。P2SA2基因的突变可导致拟南芥缺失强蓝光诱导的下胚轴向光弯曲反应。在p2sa2突变体背景下,P2SA2基因超表达可恢复强蓝光诱导的拟南芥下胚轴向光弯曲。该结果将为强蓝光下PHOT2下游基因的筛选、功能鉴定和揭开PHOT2调节强蓝光诱导的下胚轴弯曲的机制提供理论基础。
| [1]Huala E, Oeller P W, Liscum E, Han I S, Larsen E, Briggs W R. Arabidopsis NPH1: a protein kinase with a putative redox-sensing domain. Science, 1997, 278: 2120–2123[2]Demarsy E, Fankhauser C. Higher plants use LOV to perceive blue light. Curr Opin Plant Biol, 2009, 12: 69–74[3]Christie J M. Phototropin blue-light receptors. Annu Rev Plant Biol, 2007, 58: 21–45[4]Sakai T, Kagawa T, Kasahara M, Swartz T E, Christie J M, Briggs W R, Wada M, Okada K. Arabidopsis nph1 and npl1: blue light receptors that mediate both phototropism and chloroplast relocation. Proc Natl Acad Sci USA, 2001, 98: 6969–6974[5]Kinoshita T, Doi M, Suetsugu N, Kagawa T, Wada M, Shimazaki K. phot1 and phot2 mediate blue light regulation of stomatal opening. Nature, 2001, 414: 656–660[6]Kagawa T, Sakai T, Suetsugu N, Oikawa K, Ishiguro S, Kato T, Tabata S, Okada K, Wada M. Arabidopsis NPL1: a phototropin homolog controlling the chloroplast high-light avoidance response. Science, 2001, 291: 2138–2141[7]Carbonnel M D, Davis P, Roelfsema M R, Inoue S, Schepens I, Lariguet P, Geisler M, Shimazaki K, Hangarter R, Fankhauser C. The Arabidopsis PHYTOCHROME KINASE SUBSTRATE2 protein is a phototropin signaling element that regulates leaf flattening and Leaf Positioning. Plant Physiol, 2010, 152: 1391–1405[8]Kasahara M, Kagawa T, Oikawa K, Suetsugu N, Miyao M, Wada M. Chloroplast avoidance movement reduces photodamage in plant. Nature, 2002, 420: 829–832[9]Takemiya A, Inoue S, Doi M, Kinoshita T, Shimazaki K. Phototropins promote plant growth in response to blue light in low light environments. Plant Cell, 2005, 17: 1120–1127[10]Briggs W R, Christie J M. Phototropins 1 and 2: Versatile plant blue-light receptors. Trends Plant Sci, 2002, 7: 204–210[11]Motchoulski A, Liscum E. Arabidopsis NPH3: a NPH1 photoreceptor-interacting protein essential for phototropism. Science, 1999, 286: 961–964[12]Inada S, Ohgishi M, Mayama T, Okada K, Sakai T. RPT2 is a signal transducer involved in phototropic response and stomatal opening by association with phototropin 1 in Arabidopsis thaliana. Plant Cell, 2004, 16: 887–896[13]Lariguet P, Schepens I, Hodgson D, Pedmale U V, Trevisan M, Kami C, de Carbonnel M, Alonso J M, Ecker J R, Liscum E, Fankhauser C. PHYTOCHROME KINASE SUBSTRATE 1 is a phototropin1 binding protein required for phototropism. Proc Natl Acad Sci USA, 2006, 103: 10134–10139[14]Blakeslee J J, Bandyopadhyay A, Peer W A, Makam S N, Murphy A S. Relocalization of the PIN1 auxin efflux facilitator plays a role in phototropic responses. Plant Physiol, 2004, 134: 28–31[15]Stone B B, Stowe-Evans E L, Harper R M, Celaya R B, Ljung K, Sandberg G, Liscum E. Distruption in AUX1-dependent auxin influx alter hypocotyl phototropism in Arabidopsis. Mol Plant, 2008, 1: 129–144[16]Sakai T, Wada T, Ishiguro S, Okada K. RPT2: A signal transducer of the phototropic response in Arabidopsis. Plant Cell, 2000, 12: 225–236[17]Tseng T S and Briggs W R. The Arabidopsis rcn1-1 mutation impairs dephosphorylation of phot2, resulting in enhanced blue light responses. Plant Cell, 2010, 22: 392–402[18]Doi M, Shigenaga A, Emi T, Kinoshita T, Shimazaki K I. A transgene encoding a blue-light receptor, phot1, restores blue-light responses in the Arabidopsis phot1phot2 double mutant. J Exp Bot, 2004, 396: 517–523[19]赵翔, 王琳丹, 李园园, 赵青平, 张骁. PHOT2介导拟南芥下胚轴向光弯曲调节子的筛选与鉴定. 植物学报, 2014, 49: 254–261Zhao X, Wang LD, Li Y Y, Zhao Q P, Zhang X. Isolation and characterization of regulators involved in PHOT2-mediated phototropismof hypocotyls in Arabidopsis. Chin Bull Bot, 2014, 49: 254–261 (in Chinese with English abstract)[20]Zhao X, Wang Y L, Qiao X R, Wang J, Wang L D, Xu C S, Zhang X. Phototropins function in high-intensity blue light-induced hypocotyl phototropism in Arabidopsis by altering cytosolic calcium. Plant Physiol, 2013, 162: 1539–1551[21]Briggs W R, Beck C F, Cashmore A R. The phototropin family of photoreceptors. Plant Cell, 2001, 13: 993–997[22]Pedmale U V, Liscum E. Regulation of phototropic signaling in Arabidopsis via phosphorylation state changes in the phototropin 1-interacting protein NPH3. J Biol Chem, 2007, 282: 19992–20001[23]Harper R M, Stowe-Evans E L, Luesse D R, Muto H, Tatematsu K, Watahiki M K, Yamamoto K, Liscum E. The NPH4 locus encodes the auxin response factor ARF7, a conditional regulator of differential growth in aerial Arabidopsis tissue. Plant Cell, 2000, 12: 757–770[24]Liscum E, Briggs W R. Mutations of Arabidopsis in potential transduction and response components of the phototropic signaling pathway. Plant Physiol, 1996, 112: 291–296[25]de Carbonnel M, Davis P, Roelfsema M R, Inoue S, Schepens I, Lariguet P, Geisler M, Shimazaki K, Hangarter R, Fankhauser C. The Arabidopsis PHYTOCHROME KINASE SUBSTRATE2 protein is a phototropin signaling element that regulates leaf flattening and leaf positioning. Plant Physiol, 2010, 152: 1391–1405[26]Tsuchida-Mayama T, Nakano M, Uehara Y, Sano M, Fujisawa N, Okada K, Sakai T. Mapping of the phosphorylation sites on the phototropic signal transducer, NPH3. Plant Sci, 2008, 174: 626–633[27]Knauer T, Dümmer M, Landgraf F, Forreiter C. A negative effector of blue light-induced and gravitropic bending in Arabidopsis. Plant Physiol, 2011, 156: 439–447[28]Harada A, Shimazaki K. Phototropins and blue light-dependent calcium signaling in higher plants. Photochem Photobiol, 2007, 83: 102–111 |
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