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作物学报 ›› 2016, Vol. 42 ›› Issue (05): 690-695.doi: 10.3724/SP.J.1006.2016.00690
刘凌云,刘浩,赵晶,王艳霞,王棚涛
LIU Ling-Yun,LIU Hao,ZHAO Jing,WANG Yan-Xia,WANG Peng-Tao*
摘要：
叶绿素荧光动力学技术在测定叶片光合作用中光系统对光能的吸收、传递、耗散、分配等方面具有独特的作用。本研究借助叶绿素荧光成像仪, 从拟南芥EMS诱变突变体库中筛选到一株低叶绿素荧光突变体lcf3-1 (lower chlorophyll fluorescence 3-1)。遗传分析表明lcf3-1突变体为单基因隐性突变。突变基因图位克隆结果显示LCF3是PsbW的等位基因。另外, LCF3基因的T-DNA插入突变体及功能回补转基因植物的叶绿素荧光分析结果, 均证明LCF3基因突变导致拟南芥叶绿素荧光Fv/Fm值降低。进一步实验证明LCF3蛋白定位于叶绿体, 且LCF3基因在植株中普遍表达。PsbW蛋白可能细微调整PSII-LHCII超复合体的组装及稳定。
| [1]Qin X, Suga M, Kuang T, Shen J R. Photosynthesis Structural basis for energy transfer pathways in the plant PSI-LHCI supercomplex. Science, 2015, 348: 989–995 [2]Waters M T, Wang P, Korkaric M. GLK transcription factors coordinate expression of the photosynthetic apparatus in Arabidopsis. Plant Cell, 2009, 21: 1109–1128 [3]Barber J. Photosystem II: an enzyme of global significance. Biochem Soc Trans, 2006, 34: 619–631 [4]Shi L X, Schröder W P. The low molecular mass subunits of the photosynthetic supracomplex, photosystem II. Biochim Biophys Acta. 2004, 1608: 75–96 [5]Irrgang K D, Shi L X, Funk C, Schröder W P. A nuclear-encoded subunit of the photosystem II reaction center. J Biol Chem, 1995, 270: 17588–17593 [6]Lorkovi? Z J, Schröder W P, Pakrasi H B, Irrgang K D, Herrmann R G, Oelmüller R. Oelmuller R. Molecular characterization of PsbW, a nuclearencoded component of the photosystem-II reaction-center complex in spinach. Proc Natl Acad Sci USA, 1995, 92: 8930–8934 [7]Shi L X, Schröder W P. Compositional and topological studies of the PsbW protein in spinach thylakoid membrane. Photosynth Res, 1997, 53: 45–53 [8]Bishop C L, Purton S, Nugent J H. Molecular analysis of the Chlamydomonas nuclear gene encoding PsbW and demonstration that PsbW is a subunit of photosystem II, but not photosystem I. Plant Mol Biol, 2003, 52: 285–289 [9]Shi L X, Lorkovic Z J, Oelmuller R, Schröder W P. The low molecular mass PsbW protein is involved in the stabilization of the dimeric photosystem II complex in Arabidopsis thaliana. J Biol Chem, 2000, 275: 37945–37950 [10]Rokka A, Suorsa M, Saleem A, Battchikova N, Aro E M. Synthesis and assembly of thylakoid protein complexes: multiple assembly steps of photosystem II. Biochem J, 2005, 388: 159–168 [11]Thidholm E, Lindstrom V, Tissier C, Robinson C, Schröder W P, Funk C. Novel approach reveals localisation and assembly pathway of the PsbS and PsbW proteins into the photosystem II dimer. FEBS Lett, 2002, 513: 217–222 [12]Granvogl B, Zoryan M, Ploscher M, Eichacker L A. Localization of 13 one-helix integral membrane proteins in photosystem II subcomplexes. Anal Biochem. 2008, 383: 279–288 [13]Dobáková M, Sobotka R, Tichý M, Komenda J. Psb28 protein is involved in the biogenesis of the photosystem II inner antenna CP47 (PsbB) in the cyanobacterium Synechocystis sp. PCC 6803. Plant Physiol, 2009, 149:1076–1086 [14]García-Cerdán J G, Kovács L, Tóth T, Kereïche S, Aseeva E, Boekema E J, Mamedov F, Funk C, Schröder W P. The PsbW protein stabilizes the supramolecular organization of photosystem II in higher plants. Plant J, 2011, 65: 368–381 [15]Massoz S, Larosa V, Horrion B, Matagne R F, Remacle C, Cardol P. Isolation of Chlamydomonas reinhardtii mutants with altered mitochondrial respiration by chlorophyll fluorescence measurement. J Biotechnol, 2015, 215: 27–34 [16]Genty B, Briantais J M, Baker N R. The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochimica et Biophysica Acta (BBA)-General Subjects, 1989, 990: 87–92 [17]van Kooten O, Snel J F. The use of chlorophyll fluorescence nomenclature in plant stress physiology. Photosynth Res, 1990, 25: 147–150 [18]Clough S J, Bent A F. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J, 1998, 16: 735–743 [19]Yoo S D, Cho Y H, Sheen J. Arabaidopsis mesophyll protoplasts: A versatile cell system for transient gene expression analysis. Nat Prot, 2007, 2: 1565–1572 [20]Wang P T, Liu H, Hua H J, Wang L, Song C P. A vacuole localized β-glucosidase contributes to drought tolerance in Arabidopsis. Chin Sci Bull, 2011, 56: 3538–3546 [21]Sun Q, Emanuelsson O, van Wijk K J. Analysis of curated and predicted plastid subproteomes of Arabidopsis. Subcellular compartmentalization leads to distinctive proteome properties. Plant Physiol, 2004, 135: 723–734 [22]Baker N R. Chlorophyll fluorescence: a probe of photosynthesis in vivo. Annu Rev Plant Biol, 2008, 59: 89–113 [23]Murchie E H, Lawson T. Chlorophyll fluorescence analysis: a guide to good practice and understanding some new applications. J Exp Bot, 2013, 64: 3983–3998 [24]Maxwell K, Johnson G N. Chlorophyll fluorescence--a practical guide. J Exp Bot, 2000, 51: 659–668
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