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作物学报 ›› 2016, Vol. 42 ›› Issue (05): 684-689.doi: 10.3724/SP.J.1006.2016.00684

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

一个水稻黄绿叶突变基因的定位和遗传研究

初志战1,郭海滨2,刘小林3,陈远玲1,刘耀光1,*   

  1. 1 华南农业大学生命科学学院 / 亚热带农业生物资源保护与利用重点实验室, 广东广州 510642; 2 华南农业大学公共基础课实验教学中心, 广东广州 510642; 3 宜春学院, 江西宜春 336000
  • 收稿日期:2015-09-25 修回日期:2016-01-11 出版日期:2016-05-12 网络出版日期:2016-02-18
  • 通讯作者: 刘耀光, E-mail: ygliu@scau.edu.cn
  • 作者简介:刘耀光, E-mail: ygliu@scau.edu.cn
  • 基金资助:

    本研究由亚热带农业生物资源保护与利用国家重点实验室开放课题(SKL-CUSAb-2013-04),江西省教育厅科技计划项目(GJJ14707)和广东省自然科学基金-博士启动项目(2015A030310485)资助。

Genetic Analysis and Gene Mapping of a Yellow-green Leaf Mutant in Rice

CHU Zhi-Zhan1,GUO Hai-Bin2,LIU Xiao-Lin3,CHEN Yuan-Ling1,LIU Yao-Guang1,*   

  1. 1 College of Life Sciences, South China Agricultural University / State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangzhou 510642, China; 2 Center of Experimental Teaching for Common Basic Course, South China Agricultural University, Guangzhou 510642, China; 3 Yichun University, Yichun 336000, China
  • Received:2015-09-25 Revised:2016-01-11 Published:2016-05-12 Published online:2016-02-18
  • Contact: Liu Yaoguang, E-mail: ygliu@scau.edu.cn
  • About author:Liu Yaoguang, E-mail: ygliu@scau.edu.cn
  • Supported by:

    This study was supported by the Open Fund Project of State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources (SKL-CUSAb-2013-04), the Science and Technology Program from Education Department of Jiangxi Province (GJJ14707), and the Natural Science Fund of Guangdong Province-the Launch Program for Doctor (2015A030310485).

摘要:

从粳稻品种日本晴经60Co诱变的M1材料中发现一个黄绿叶突变体, 其叶片从萌发到三叶前期表现白化, 三叶后期开始转为黄绿叶, 直到衰老。遗传分析表明, 该突变表型受一对隐性核基因控制, 将该黄绿叶突变体暂定名为ygl8951。与野生型相比, ygl8951的叶绿素含量与类胡萝卜素含量显著降低。电子显微镜观察表明ygl8951内叶绿体数量明显减少, 叶绿体内没有基粒类囊体, 只有类似间质类囊体结构。基因表达定量分析表明, 突变体中光系统I和光系统II基因表达水平明显下调, 核糖体结构基因和质体编码的RNA聚合酶亚基基因表达明显上调。利用ygl8951与籼稻品种黄华占杂交获得的F2分离群体, 将该基因定位于水稻第6染色体上的In/Del标记607489与607611之间, 物理距离191 kb的范围内, 通过分析确认该基因为一个新的调控叶色的基因。

关键词: 水稻, 黄绿叶, 基因定位, 遗传分析

Abstract:

A yellow-green leaf rice mutant, temporarily named as ygl8951 (yellow-green leaf 8951), was identified from 60Co γ-ray radiation mutation in japonica rice varietyNipponbare. The mutant showed albino phenotype from germination to 3-leaf-stage, then turned yellow-green phenotype till apoptosis. The contents of chlorophyll and carotenoid were obviously decreased in ygl8951 compared with the wild type. Electron microscope observation showed that no grana thylakoids but some stroma thylakoids-like structures were found in chloroplast of ygl8951 mutant. The expression levels of some genes involved in photosystem Iand photosystem II were dramatically decreased, while the ribosomal and RNA polymerase genes in chloroplast were increased in ygl8951 mutant compared with the wild type. Mapping-based cloning was used to identify the ygl8951 locus using an F2 population from a crossing between the mutant and Huanghuazhan. The result showed that the mutated locus was located in a 191 kb region on chromosome 6, which was assumed to be a new gene controlling leaf color.

Key words: Rice, Yellow-green leaf, Gene mapping, Genetic analysis

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