欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2016, Vol. 42 ›› Issue (02): 255-264.doi: 10.3724/SP.J.1006.2016.00255

• 耕作栽培·生理生化 • 上一篇    下一篇

1,2,4-三氯苯对2个不同耐性水稻品种光合特性与产量的影响

李玉1,陈璐1,严凯1,孙影1,殷毅凡1,丁秀文1,戴其根1,2,*,张洪程1,2   

  1. 1扬州大学江苏省作物遗传生理重点实验室, 江苏扬州225009; 2扬州大学农业部长江流域稻作技术创新中心, 江苏扬州225009
  • 收稿日期:2015-05-22 修回日期:2015-11-20 出版日期:2016-02-12 网络出版日期:2015-12-07
  • 通讯作者: 戴其根, E-mail: qgdai@yzu.edu.cn, Tel: 13701442683
  • 基金资助:

    本研究由国家自然科学基金项目(31271639)和国家公益性行业(农业)科研专项(201303102)资助。

Effects of 1,2,4-Trichlorobenzene on Photosynthetic Characteristics of Flag Leaf during Grain Filling Stage and Grain Yield of Two Rice Cultivars

LI Yu1,CHEN Lu1,YAN Kai1,SUN Ying1,YIN Yi-Fan1,DING Xiu-Wen1,DAI Qi-Gen1,2,*,ZHANG Hong-Cheng1, 2   

  1. 1 Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou University, Yangzhou 225009, China; 2 Innovation Center of Rice Cultivation Technology in Yangtze River Valley, Ministry of Agriculture, Yangzhou 225009, China
  • Received:2015-05-22 Revised:2015-11-20 Published:2016-02-12 Published online:2015-12-07
  • Contact: 戴其根, E-mail: qgdai@yzu.edu.cn, Tel: 13701442683
  • Supported by:

    This study was support by the National Natural Science Foundation of China (31271639) and the Special Fund for Agro-scientific Research in the Public Interest (201303102).

RichHTML

PDF

776

被引次数

1

摘要：

为明确不同类型水稻品种的响应规律，为水稻高产稳产、安全生产提供依据。在盆栽土培条件下，研究了5种1,2,4-三氯苯(TCB)浓度(0、10、20、40、80 mg kg-1土)对2个水稻品种(宁粳1号，敏感；扬辐粳8号，耐性)灌浆期剑叶光合特性与产量的影响。结果表明，TCB的影响品种间差异显著。低浓度TCB (10 mg kg-1)下，扬辐粳8号的株高、鲜重和产量显著增加，叶绿素含量、净光合速率、胞间CO2浓度、蒸腾速率和qN略有升高，但与对照的差异不显著，而Fv/Fm和Fv/Fo略有下降；宁粳1号的净光合速率、胞间CO2浓度、蒸腾速率、ΦPSII、Fv/Fm、Fv/Fo、qp和产量略有下降，气孔导度显著降低。在20 mg kg-1 TCB处理下，宁粳1号的光合参数、产量、株高、地上部和地下部鲜重均显著降低，而扬辐粳8号略有降低，表现出较强耐性；在中高浓度TCB (40 mg kg-1，80 mg kg-1)处理下，2个水稻品种的光合特性、株高、鲜重和产量均受显著抑制，且以宁粳1号的降幅较大。TCB对水稻光合特性与产量的影响不仅与其浓度有关，且存在显著品种差异，耐性水稻品种表现出低浓度TCB处理对其株高、鲜重、叶绿素含量、光合特性及其产量有一定的促进作用，在中高浓度TCB处理时表现出较强的耐性，受胁迫程度小

关键词: 水稻, 灌浆期, 1,2,4-三氯苯, 品种, 光合特性, 产量

Abstract:

A pot experiment was conducted, using two rice cultivars Ningjing 1 (TCB sensitive) and Yangfujing 8 (TCB tolerant), with five concentration treatments (0, 10, 20, 40, and 80 mg TCB per kg dry soil) to explore the responses of two rice cultivars to TCB, and provide the basis for the high, stable, and safe production of rice. The results indicated that significant differences were found in the effects of TCB treatments on grain yield and photosynthetic parameters of flag leaf between the two cultivars. Plant height, fresh weight, and yield were significantly increased, chlorophyll content, net photosynthetic rate, intercellular CO2 concentration, transpiration rate, and qn were slightly increased, while Fv/Fm and Fv/Fo were slightly decreased in the low TCB concentration (10 mg kg-1) treatment of Yangfujing 8. Under the same condition, the net photosynthetic rate, CO2 intercellular concentration, transpiration rate, ΦPSII, Fv/Fm, Fv/Fo, qp, yield in Ningjing 1 were slightly declined with significant reduction of stomatal conductance. The photosynthetic characteristics, yield, plant height, fresh weight in Ningjing 1 were decreased significantly, while Yangfujing 8 showed more resistance and adaptation to TCB at 20 mg kg-1. Both cultivars showed significant decrease in growth, photosynthesis and yield in treatments with high TCB concentrations (40 and 80 mg kg-1), with the greater decrements in Ningjing 1. The effect of TCB on photosynthetic characteristics and yield of rice was not only related to TCB concentration, but also to cultivars. Low TCB levels slightly promoted the plant height, fresh weight, chlorophyll content, photosynthetic characteristics and rice yield in Yangfujing 8 that showed stronger tolerance to TCB than Ningjing 1, under high TCB concentrations (40 and 80 mg kg-1). 

Key words: Rice, Grain-filling stage, 1,2,4-three chlorobenzene, Cultivars, Photosynthetic characteristics, Yield

[1] 宋玉芳, 周启星, 许华夏, 任丽萍, 宋雪英, 龚平. 菲、芘、1,2,4-三氯苯对土壤高等植物根伸长抑制的生态毒性效应. 生态学报, 2002, 21: 1945–1950



Song Y F, Zhou Q X, XU H X, Ren L P, Song X Y, Gong P. Evolution characteristics of grain yield and plant type for mid season indica rice cultivars. Acta Ecol Sin, 2002, 21:1945–1950 (in Chinese with English abstract)



[2] Diaz J, Rendueles M, Diaz M. 1,2,4-trichlorobenzene flow characteristics in saturated homogeneous and stratified porous media. Water Air Soil Pollut, 2006, 177: 3–17



[3] 周文敏, 傅德黔, 孙宗光. 水中优先控制污染物黑名单. 中国环境监测, 1990, 6(4): 1–3



Zhou W M, Fu D Q, Sun Z G. Determination of black list of China’s priority pollutants in water. Res Environ Sci, 1990, 6(4): 1–3 (in Chinese with English abstract)



[4] Wang M J, Jones K C. Analysis of chlorobenzenes in sewage sludge by capillary gas chromatography. Chemosphere, 1991, 23: 677–691



[5] Rogers H R, Campbell J A, Crathorne B, Dobbs A J. The occurrence of chlorobenzenes and permethrins in twelve U. K. sewage sludges. Water Res, 1989, 23(7): 913–921



[6] Wild S R, Jones K C. Organic chemicals entering agricultural soils in sewage sludges: screening for their potential to transfer to crop plants and livestock. Sci Total Environ, 1992, 119: 85–119



[7] Wang M J, Jones K C. Behavior and fate of chlorobenzenes in spiked and sewage sludge-amended soil. Environ Sci Technol, 1994, 28: 1843–1852



[8] 莫测辉, 蔡全英, 吴启堂, 李桂荣, 王伯光, 田凯. 城市污泥中有机污染物的研究进展. 农业环境保护, 2001, 20: 273–276



Mo C H, Cai Q Y, Wu Q T, Li G R, Wang B G, Tian K. Research advances on organic pollutants in municipal sludge. Agric Environ Prot, 2001, 20: 273–276 (in Chinese with English abstract)



[9] 张丽珊, 于殿臣, 刘海玲, 姚家彪, 朱岩, 尹昭华, 姜萍, 可夫. 慢速渗滤土地处理系统对沈阳西部城市污水有机污染物净化效果的研究. 应用生态学报, 1992, 3: 355–362



Zhang L S, Yu D C, Liu H L, Yao J B, Zhu Y, Yin Z H, Jiang P, Ke F. Purification effect of slow rate land treatment system on organic pollutants in municipal wastewater from west Shenyang. Chin J Appl Ecol, 1992, 3: 355–362 (in Chinese with English abstract)



[10] 蔡全英, 莫测辉, 吴启堂, 王伯光. 城市污泥堆肥处理过程中有机污染物的变化. 农业环境保护, 2001, 20(3): 186–189



Cai Q Y, Mo C H, Wu Q T, Wang B G. Variation of organic pollutants in treatment of sewage sludge during composting. Agric Environ Prot, 2001, 20(3): 186–189 (in Chinese with English abstract)



[11] 杜青平, 贾晓珊, 袁保红. 1,2,4-三氯苯对水稻种子萌发及幼苗生长的毒性机理. 应用生态学报, 2006, 17: 2185–2188



Du Q P, Jia X S, Yuan B H. Toxic effects of 1,2,4-trichlorobenzene on rice seed germ ination and seedling growth. Chin J Appl Ecol, 2006, 17: 2185–2188 (in Chinese with English abstract) 



[12] 王泽港, 葛才林, 万定珍, 郦志文, 罗时石, 杨建昌. 1,2,4-三氯苯和萘对水稻幼苗生长的影响. 农业环境科学学报, 2006, 25: 1402–1407



Wang Z G, Ge C L, Wan D Z, Li Z W, Luo S S, Yang J C. Effects of 1,2,4-trichlorobenzene and Naphthalene on growth of rice seedling. J Agro-Environ Sci, 2006, 25: 1402–1407 (in Chinese with English abstract)



[13] 张国良, 陈文军, 仇利民, 孙国荣, 戴其根, 张洪程. 不同基因型水稻苗期对1,2,4-三氯苯胁迫的生理响应. 作物学报, 2009, 35: 733–740



Zhang G L, Chen W J, Qiu L M, Sun G R, Dai Q G, Zhang H C. Physiological response to 1,2,4-trichlorobenzene stress of different rice genotypes in seedlings. Acta Agron Sin, 2009, 35:733–740 (in Chinese with English abstract)



[14] 陈文军, 张国良, 孙国荣, 戴其根, 张洪程, 陶金飞, 孙洁, 严林锋. 水稻耐1,2,4-三氯苯胁迫基因型的苗期筛选. 中国农业科学, 2008, 27: 1003–1008



Chen W J, Zhang G L, Sun G R, Dai Q G, Zhang H C, Tao J F, Sun J, Yan L F. Screening of tolerant rice genotypes to 1,2,4-trichlorobenzene stress at seedling stage. J Agro-Environ Sci, 2008, 27: 1003–1008 (in Chinese with English abstract)



[15] 丁秀文, 张国良, 戴其根, 朱青. 1, 2, 4-三氯苯胁迫对水稻分蘖盛期植株生长和生理特性的影响. 作物学报, 2014, 40: 487–496



Ding X W , Zhang G L , Dai Q G, Zhu Q. Effects of 1,2,4-trichlorobenzene on growth and physiological characteristics of rice at maximum tillering stage. Acta Agron Sin, 2014, 40: 487–496 (in Chinese with English abstract)



[16] 王泽港, 万定珍, 杨亚春, 葛才林, 马飞, 杨建昌. 1, 2, 4-三氯苯和萘对水稻产量及品质的影响. 中国水稻科学, 2006, 20: 295–300



Wang Z G, Wan D Z, Yang Y C, Ge C L, Ma F, Yang J C. Effects of 1,2,4-trichlorobenzene and naphthalene on grain yield and quality of rice. Chin J Rice Sci, 2006, 20: 295–300 (in Chinese with English abstract)



[17] 洛育, 张凤鸣, 白良明, 孙世臣, 姜辉, 张玉华, 耿立清. 硝基苯污染对水稻生长发育及稻米安全性的影响. 中国农学通报, 2009, 25(24): 468–471



Luo Y, Zhang F M, Bai L M, Sun S C, Jiang H, Zhang Y H, Geng L Q. Effect of contamination of nitrobenzene on rice growth and food safety of rice. Chin Agric Sci Bull, 2009, 25(24): 468–471 (in Chinese with English abstract)



[18] 王泽港, 骆剑峰, 高红明, 万定珍, 葛才林, 罗时石, 杨建昌. 1,2,4-三氯苯和萘对水稻抽穗期叶片光合特性的影响. 中国农业科学, 2005, 38: 1113–1119



Wang Z G, Luo J F, Gao H M, Wan D Z, Ge C L, Luo S S, Yang J C. Effects of 1,2,4-trichlorobenzene and naphthalene stress on photosynthetic characteristics of rice at heading period. Sci Agric Sin, 2005, 38: 1113–1119 (in Chinese with English abstract)



[19] 潘瑞炽. 植物生理学(第五版). 北京: 高等教育出版社, 2004. pp 56–57, 84–86



Pan R Z. Plant Physiology (Fifth Edn). Beijing: Higher Education Press, 2004. pp 56–57, 84–86 (in Chinese)



[20] 丁艳, 葛才林, 王泽港, 杜庆才. 小麦幼苗对镉和1,2,4-三氯苯污染的响应. 中国农业大学学报, 2011, 16(3): 48–52



Ding Y , Ge C L, Wang Z G, Du Q C. Response of Cd and 1,2,4-trichlorobenzene pollutants on growth of wheat seedlings. J China Agric Univ, 2011, 16(3): 48–52 (in Chinese with English abstract)



[21] Schreiber U, Bilger W, Neubauer C. Chlorophyll fluorescence as a non-intrusive indicator for rapid assessment of in vivo photosynthesis. In: Schulze E D, Caldwell M M, eds. Ecophysiology of Photosynthesis. Berlin: Springer-Verlag, 1994. pp 49–70



[22] Ralph J, Gademann R. Rapid light curves: a powerful tool to assess photosynthetic activity. Aquatic Bot, 2005, 82: 222–237



[23] 徐应明, 袁志华, 李军幸, 戴晓华. 硝基苯和氯苯灌溉对春小麦品质影响研究. 灌溉排水学报, 2004, 23(3): 17–19



Xu Y M, Yuan Z H, Li J X, Dai X H. Research nitrobenzene and chlorobenzene irrigation on spring wheat quality. J Irrig Drain, 2004, 23(3): 17–19 (in Chinese)



[24] 刘宛, 孙铁珩, 李培军, 周启星, 台培东, 张春桂, 许华夏, 张海荣. 三氯苯胁迫对大豆下胚轴膜脂过氧化的影响. 农业环境保护, 2002, 21: 413–416



Liu W, Sun T H, Li P J, Zhou Q X, Tai P D, Zhang C G, Xu H X, Zhang H R. Effect of 1,2,4-Trichlorobenzene stress on membrane lipid peroxidation in soybean hypocotyls. Agro-Environ Prot, 2002, 21: 413–416 (in Chinese with English abstract)



[25] 刘宛, 宋玉芳, 周启星, 李培军, 孙铁珩, 姚德明. 氯苯胁迫对小麦种子发芽和幼苗生长的影响. 农业环境保护, 2001, 20(2): 65–68



Liu W, Song Y F, Zhou Q X, Li P J, Sun T H, Yao D M. Effect of chlorobenzene-stress on seed germination and seedling growth of wheat. Agro-Environ Prot, 2001, 20(2): 65–68 (in Chinese with English abstract)



[26] Ben-asher J, Tsuyuki I, Bravdo B A, Sagih M. Irrigation of grapevines with saline water: I. Leaf area index, stomatal conductance, transpiration and photosynthesis. Agric Water Manag, 2006, 83: 13–21



[27] Berry J A, Downton W J S. Environmental regulation of photosynthesis. In: Govind J. Photosynthesis: Vol. II. New York: Academic Press, 1982. pp 263–345



[28] 葛江丽, 石雷, 谷卫彬, 唐宇丹, 张金政, 姜闯道, 任大明. 盐胁迫条件下甜高粱幼苗的光合特性及光系统II功能调节. 作物学报, 2007, 33: 1272–1278



Ge J L, Shi L, Gu W B, Tang Y D, Zhang J Z, Jiang C D, Ren D M. Photosynthetic characteristics and the regulation of photosystem II function in salt stressed sweet sorghum seedlings. Acta Agron Sin, 2007, 33: 1272–1278 (in Chinese with English abstract)



[29] 温国胜, 田海涛, 张明如, 蒋文伟. 叶绿素荧光分析技术在林木培育中的应用. 应用生态学报, 2006, 17: 1973–1977



Wen G S, Tian H T, Zhang M R, Jiang W W. Application of chlorophyll fluorescence analysis in forest tree cultivation. Chin J Appl Ecol, 2006, 17: 1973–1977



[30] 王北洪, 黄木易, 马智宏, 王纪华. 条锈病对冬小麦叶绿素荧光、光合及蒸腾作用的影响. 华北农学报, 2004, 19: 92–94



Wang B H, Huang M Y, Ma Z H, Wang J H. Effect of stripe rust on chlorophyll fluorescence and photosynthesis of winter wheat. Agta Agric Boreali-Sin, 2004, 19: 92–94 (in Chinese with English abstract)



[31] Kiang N Y, Siefert J, Govindjee, Blankenship R E. Spectral signatures of photosynthesis. I. Review of earth organisms. Astrobiology, 2007, 7: 222–251



[32] 张雪芬, 陈怀亮, 郑有飞, 邹春辉, 陈东, 付祥建. 冬小麦冻害遥感监测应用研究. 南京气象学院学报, 2006, 29(1): 94–100



Zhang X F, Chen H L, Zheng Y C, Zhou C H, Chen D, Fu X J. Monitoring the freezing injury of winter wheat by remote sensing. J Nanjing Inst Meteorol, 2006, 29(1): 94–100 (in Chinese with English abstract)



[33] Rizza F, Pagani D, Stanca A M, Cattivelli L. Use of chlorophyll fluorescence to evaluate the cold acclimation and freezing tolerance of winter and spring oats. Plant Breed, 2001, 20: 389–396



[34] Grzesiak M, Rzepka A, Czyczylo-Mysza I, Hura T, Dziurka M. Emission and excitation spectra of drought-stressed and non-stressed maize and triticale seedling leaves. Zeszyty Problemowe Postepow Nauk Rolniczych, 2008, 524: 213–220



[35] 翁永玲，宫鹏. 土壤盐渍化遥感应用研究进展. 地理科学，2006, 26: 369–375



Weng Y L, Gong P. Research progress of application of soil salinization remote sensing. Sci Geogr Sin, 2006, 26: 369–375



[36] Pu R L, Kelly M, Anderson G L, Gong P. Using CASI hyperspectral imagery to detect mortality and vegetation stress associated with a new hardwood forest disease. Photogram Eng Remote Sens, 2008, 74: 65–75



[37] Huang W J, Lamb D W, Niu Z, Zhang Y J, Liu L Y, Wang J H. Identification of yellow rust in wheat using in-situ spectral reflectance measurements and airborne hyperspectral imaging. Precision Agric, 2007, 8: 187–197



[38] 张雷明, 上官周平, 毛明策, 于贵端. 长期施氮对旱地小麦灌浆期叶绿素荧光参数的影响. 应用生态学报,2003, 14: 695–698



Zhang L M, Shang-Guan Z P, Mao M C,Yu G D. Effects of long ter m application of nitrogen fertilizer on leaf chlorophyll fluorescence of upland winter wheat. Chin J Appl Ecol, 2003, 14: 695–698 (in Chinese with English abstract)



[39] 王荣富, 张云华, 钱立生, 于江龙. 超级杂交稻两优培九及其亲本的光氧化特性. 应用生态报, 2003, 14: 1309–1312



Wang R F, Zhang Y H, Qian L S, Yu J L. Photooxidation characteristics of super hybrid rice “Liangyoupeijiu” and its parents. Chin J Appl Ecol, 2003, 14: 1309–1312 (in Chinese with English abstract)



[40] 李晓, 冯伟, 曾晓春. 叶绿素荧光分析技术及应用进展. 西北植物学报, 2006, 26: 2186 –2196



Li X, Feng W, Zeng X C. Advances in chlorophyll fluorescence analysis and its uses. Acta Bot Boreali-Occident Sin, 2006, 26: 2186–2196



[41] Subhash N, Wenzel O, Lichtenthaler H K. Changes in blue-green and chlorophyll fluorescence emission and fluorescence ratios during senescence of tobacco plants. Remote Sens Environ, 1999, 69: 215–213



[42] Van Kooten O, Snel J F H. The use of chlorophyll nomenclature in plant stress physiology. Photosynth Res, 1990, 25: 147–150
[1] 田甜, 陈丽娟, 何华勤. 基于Meta-QTL和RNA-seq的整合分析挖掘水稻抗稻瘟病候选基因[J]. 作物学报, 2022, 48(6): 1372-1388.
[2] 郑崇珂, 周冠华, 牛淑琳, 和亚男, 孙伟, 谢先芝. 水稻早衰突变体esl-H5的表型鉴定与基因定位[J]. 作物学报, 2022, 48(6): 1389-1400.
[3] 周文期, 强晓霞, 王森, 江静雯, 卫万荣. 水稻OsLPL2/PIR基因抗旱耐盐机制研究[J]. 作物学报, 2022, 48(6): 1401-1415.
[4] 郑小龙, 周菁清, 白杨, 邵雅芳, 章林平, 胡培松, 魏祥进. 粳稻不同穗部籽粒的淀粉与垩白品质差异及分子机制[J]. 作物学报, 2022, 48(6): 1425-1436.
[5] 王丹, 周宝元, 马玮, 葛均筑, 丁在松, 李从锋, 赵明. 长江中游双季玉米种植模式周年气候资源分配与利用特征[J]. 作物学报, 2022, 48(6): 1437-1450.
[6] 王旺年, 葛均筑, 杨海昌, 阴法庭, 黄太利, 蒯婕, 王晶, 汪波, 周广生, 傅廷栋. 大田作物在不同盐碱地的饲料价值评价[J]. 作物学报, 2022, 48(6): 1451-1462.
[7] 颜佳倩, 顾逸彪, 薛张逸, 周天阳, 葛芊芊, 张耗, 刘立军, 王志琴, 顾骏飞, 杨建昌, 周振玲, 徐大勇. 耐盐性不同水稻品种对盐胁迫的响应差异及其机制[J]. 作物学报, 2022, 48(6): 1463-1475.
[8] 杨欢, 周颖, 陈平, 杜青, 郑本川, 蒲甜, 温晶, 杨文钰, 雍太文. 玉米-豆科作物带状间套作对养分吸收利用及产量优势的影响[J]. 作物学报, 2022, 48(6): 1476-1487.
[9] 陈静, 任佰朝, 赵斌, 刘鹏, 张吉旺. 叶面喷施甜菜碱对不同播期夏玉米产量形成及抗氧化能力的调控[J]. 作物学报, 2022, 48(6): 1502-1515.
[10] 徐田军, 张勇, 赵久然, 王荣焕, 吕天放, 刘月娥, 蔡万涛, 刘宏伟, 陈传永, 王元东. 宜机收籽粒玉米品种冠层结构、光合及灌浆脱水特性[J]. 作物学报, 2022, 48(6): 1526-1536.
[11] 李祎君, 吕厚荃. 气候变化背景下农业气象灾害对东北地区春玉米产量影响[J]. 作物学报, 2022, 48(6): 1537-1545.
[12] 杨建昌, 李超卿, 江贻. 稻米氨基酸含量和组分及其调控[J]. 作物学报, 2022, 48(5): 1037-1050.
[13] 石艳艳, 马志花, 吴春花, 周永瑾, 李荣. 垄作沟覆地膜对旱地马铃薯光合特性及产量形成的影响[J]. 作物学报, 2022, 48(5): 1288-1297.
[14] 杨德卫, 王勋, 郑星星, 项信权, 崔海涛, 李生平, 唐定中. OsSAMS1在水稻稻瘟病抗性中的功能研究[J]. 作物学报, 2022, 48(5): 1119-1128.
[15] 朱峥, 王田幸子, 陈悦, 刘玉晴, 燕高伟, 徐珊, 马金姣, 窦世娟, 李莉云, 刘国振. 水稻转录因子WRKY68在Xa21介导的抗白叶枯病反应中发挥正调控作用[J]. 作物学报, 2022, 48(5): 1129-1140.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2