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作物学报 ›› 2019, Vol. 45 ›› Issue (10): 1503-1510.doi: 10.3724/SP.J.1006.2019.91011

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

小麦新种质CH1357抗白粉病遗传分析及染色体定位

陈芳1,乔麟轶2,3,李锐2,刘成4,李欣2,3,郭慧娟2,张树伟2,常利芳2,李东方5,阎晓涛2,任永康2,张晓军2,3,*(),畅志坚2,3,*()   

  1. 1山西大学生命科学学院, 山西太原 030006
    2山西省农业科学院作物科学研究所 / 作物遗传与分子改良山西省重点实验室, 山西太原 030031
    3农业部黄土高原作物基因资源与种质创制重点实验室, 山西太原 030031
    4山东省农业科学院作物研究所, 山东济南 250100
    5新疆生产建设兵团第六师农业科学研究所, 新疆五家渠 831300
  • 收稿日期:2019-01-18 接受日期:2019-05-12 出版日期:2019-10-12 网络出版日期:2019-09-10
  • 通讯作者: 张晓军,畅志坚
  • 基金资助:
    本研究由国家重点研发计划项目(2017YFD0100600);山西省农业科学院项目(YGG17123);山西省农业科学院项目(YCX2018D2YS01);山东省自然科学基金项目(ZR2017MC004);山西省重点研发计划项目(201703D211007);山西省重点研发计划项目(201803D221018-5);山西省重点研发计划项目(201803D421020);山西省重点科技创新平台资助(201605D151002)

Genetic analysis and chromosomal localization of powdery mildew resistance gene in wheat germplasm CH1357

CHEN Fang1,QIAO Lin-Yi2,3,LI Rui2,LIU Cheng4,LI Xin2,3,GUO Hui-Juan2,ZHANG Shu-Wei2,CHANG Li-Fang2,LI Dong-Fang5,YAN Xiao-Tao2,REN Yong-Kang2,ZHANG Xiao-Jun2,3,*(),CHANG Zhi-Jian2,3,*()   

  1. 1College of Life Science, Shanxi University, Taiyuan 030006, Shanxi, China
    2Institute of Crop Science, Shanxi Academy of Agricultural Sciences / Shanxi Key Laboratory of Crop Genetics and Molecular Improvement, Taiyuan 030006, Shanxi, China
    3Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau of Ministry of Agriculture, Taiyuan 030006, Shanxi, China
    4Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong, China
    5Sixth Agricultural Division Agricultural Science Research Institute of Xinjiang Production and Construction Crops, Wujiaqu 831300, Xinjiang, China
  • Received:2019-01-18 Accepted:2019-05-12 Published:2019-10-12 Published online:2019-09-10
  • Contact: Xiao-Jun ZHANG,Zhi-Jian CHANG
  • Supported by:
    This study was supported by the National Key Research and Development Program of China(2017YFD0100600);Shanxi Academy of Agricultural Sciences(YGG17123);Shanxi Academy of Agricultural Sciences(YCX2018D2YS01);the National Natural Science Foundation of Shandong Province(ZR2017MC004);the Key Research and Development Program of Shanxi Province(201703D211007);the Key Research and Development Program of Shanxi Province(201803D221018-5);the Key Research and Development Program of Shanxi Province(201803D421020);the Key Scientific and Technological Innovation Platform(201605D151002)

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摘要：

白粉病是影响小麦产量和品质的一种主要病害。小偃麦衍生品系CH1357对白粉病具有较好的成株抗性, 苗期对27个菌株表现为免疫或高抗, 是一个高抗白粉病的优异抗源。为了明确其抗白粉病基因在染色体上的位置, 对台长29/CH1357和绵阳11/CH1357的F1、BC1及F2:3家系进行了遗传分析, 并利用分离群体分组分析法(bulked segregant analysis, BSA)将其初步定位。CH1357的白粉病抗性受1对显性核基因控制, 位于染色体5DS, 暂命名为PmCH1357。其侧翼连锁标记为Xcfd81和Xbwm8, 在2个作图群体台长29/CH1357和绵阳11/CH1357中的遗传距离分别为2.0 cM/11.3 cM和1.5 cM/8.9 cM。PmCH1357与5DS染色体上已报道的其他抗白粉病基因抗谱不同, 可能是一个新的抗源。

关键词: 白粉病抗性, SSR标记, 连锁图谱, 基因定位, Pm2

Abstract:

Powdery mildew is a serious disease affecting yield and quality of wheat. Wheat-Thinopyrum imtermedium introgression line CH1357 is highly resistant to Blumeria graminis f. sp. tritici (Bgt) at the adult plant stage and immune or highly resistant to 27 Bgt isolates at the seedling stage. Two mapping populations (F1, BC1, and F2:3) derived from Taichang 29/CH1357 and Mianyang 11/CH1357 were used to map the powdery mildew resistance gene by the bulked segregant analysis. The powdery mildew resistance in CH1357 was controlled by a single dominant gene, temporarily named PmCH1357. This gene was located on the short arm of chromosome 5D and linked to SSR markers Xcfd81 and Xbwm8, with genetic distances of 2.0 cM and 11.3 cM in the Taichang 29/CH1357 population and 1.5 cM and 8.9 cM in the Mianyang 11/CH1357 population, respectively. PmCH1357 differs from other Pm genes reported on chromosome 5DS in resistance spectrum, which may be a new source of resistance.

Key words: resistance to powdery mildew, SSR markers, linkage map, gene mapping, Pm2

表1

CH1357、台长29 (TC29)和绵阳11 (MY11)苗期对32个白粉菌株的抗性反应"

白粉菌株Bgt 材料Material 白粉菌株Bgt 材料Material 白粉菌株Bgt 材料Material
CH1357 TC29 MY11 CH1357 TC29 MY11 CH1357 TC29 MY11
B09 0 4 4 B33 0 4 4 B50 3 4 4
B13 0 4 4 B37 0 4 4 B51 2 4 4
B14 4 4 4 B38 1 4 4 B52 0 4 3
B15 0 4 4 B39 0 4 4 B55 0 4 4
B17 0 4 4 B40 0 4 4 B56 0 4 4
B21 0 4 4 B41 0 4 4 B57 0 4 4
B22 0 4 4 B42 0 4 4 E09 0 4 4
B23 0 4 3 B44 0 4 4 E18 4 4 4
B25 0 4 4 B46 0 4 3 E21 0 4 4
B28 0 4 4 B48 0 4 4 E23-1 0 4 4
B30 0 4 4 B49 0 4 4 - - - -

表2

2个作图群体不同世代对白粉菌株E09的苗期抗性和分离比率"

亲本/组合
Parent/cross		 对白粉菌株E09的侵染类型 Infection types to Bgt isolates E09 理论比率
Theoretical ratio
R:S		 χ2值
χ2 value		 P值
P-value
0 0; 1 2 3 4
CH1357 (P1) 12 8
台长29 Taichang 29 (P2) 5 15
绵阳11 Mianyang 11 (P3) 5 14
P2/P1 (F1) 13 7
P1/P2 (F1) 15 5
P2/P1//P2 (BC1) 14 10 4 2 12 17 1:1 0.017 0.896
P3/P1 (F1) 14 5
P1/P3 (F1) 16 4
P3/P1//P3 (BC1) 12 14 2 1 12 15 1:1 0.071 0.789

表3

2个F2:3作图群体对白粉菌株E09的苗期抗性"

F2: 3家系表现型
Phenotype of F2:3 lines		 杂交组合 Hybrid cross
CH1357/Taichang 29 CH1357/Mianyang 11
实际值 Actual value 期望值 Expected value 实际值 Actual value 期望值 Expected value
纯合抗病株 Resistant plants 120 120.75 108 102.75
抗性分离株 Heterozygous plants 231 241.50 208 205.50
纯合感病株 Susceptible plants 132 120.75 95 102.75
合计 Total 483 411
χ2 (1:2:1) 1.509 0.883
P值P-value 0.470 0.643

图1

SSR标记Xcfd81和Xgwm190在CH1357 F2群体中的扩增结果 M: marker; Pr: 抗病亲本CH1357; Ps: 感病亲本台长29; Br: 抗病池; Bs: 感病池; 1~6: 纯合抗病株; 7~12: 纯合感病株。"

图2

PmCH1357在群体台长29/CH1357 (a)与绵阳11/CH1357 (b)中的遗传定位及其与Pm2位点其他基因的比较"

图3

CH1357中连锁标记与染色体5DS上部分已知抗白粉病基因的比较 M: marker; 1: 台长29; 2: CH1357; 3: UIka (Pm2a) ; 4: KM2939 (Pm2b); 5: 鸟麦(Pm2c)。"

表4

CH1357与染色体5DS上已知抗白粉病基因对12个白粉菌株的抗性反应比较"

品种/品系
Variety/line		 抗白粉病基因
Pm gene		 白粉菌株 Blumeria graminis tritici isolate
B13 B14 B38 B40 B41 B50 B51 B56 E09 E18 E21 E23-1
CH1357 PmCH1357 0 4 1 0 0 3 2 0 0 4 0 0
UIka/8*Cc Pm2a 0 4 3 3 1 4 3 0; 0; 4 0; 0;
KM2939 Pm2b 0 4 1 0 0; 4 0 0; 0; 1 3 0;
鸟麦Niaomai Pm2c 0 0 2 0 0 4 0 0 0; 0 0; 0;
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