玉米生长素响应因子家族基因的表达模式分析

doi:10.3724/SP.J.1006.2021.03043

摘要/Abstract

摘要：

生长素响应因子(auxin response factor, ARF)是一类重要的转录因子, 通过特异性地结合生长素响应元件调节下游靶基因的转录, 参与诸多植物生长发育过程的调控。玉米中有许多ARF家族基因, 但其表达模式有待深入研究。本研究分析了玉米ARF家族基因在不同组织器官中的表达, 发现除ARF10、ARF16和ARF34组成型表达外, 其余32个ARF基因的表达水平在生殖器官中要明显高于营养器官。对ARF基因启动子区的顺式作用元件分析显示, 28个ARF基因的启动子区含有逆境胁迫相关顺式元件, 实时定量PCR分析结果显示, 多个ARF基因分别响应冷、热、盐和渗透胁迫。研究结果不仅暗示了ARF家族基因在玉米生殖生长和非生物逆境胁迫响应中的重要性, 也为全面解析ARF基因在玉米中的生物学功能提供有用信息。

关键词: ARF, 表达, 逆境胁迫, 玉米

Abstract:

Auxin response factors (ARFs) are important transcription factors which control the expression of target genes by binding specifically to auxin response elements, and are involved in a series of developmental processes in plant species. In maize genome, dozens of ARF genes are encoded, however, there is little known on their expression patterns. In this study, the analysis on the expression level of ARF genes in diverse tissues and organs revealed that expression level of 32 ARF genes were higher in reproductive organs than that in vegetative organs, except ARF10, ARF16, and ARF34 constitutively expressed. The predicted results of cis-acting elements showed that the promoter regions of 28 ARF genes harbored the cis-regulatory elements related to abiotic stresses. Real-time quantitative PCR results indicated that expression of several ARF genes showed a response to cold, heat, and osmotic stresses, respectively. The results highlighted the importance of ARF family genes in reproductive growth and abiotic stress response, and provided useful information for the comprehensive analysis of the biological function of ARF genes in maize.

Key words: auxin response factor, expression, abiotic stress, maize (Zea mays L.)

李文兰, 李文才, 孙琦, 于彦丽, 赵勐, 鲁守平, 李艳娇, 孟昭东. 玉米生长素响应因子家族基因的表达模式分析[J]. 作物学报, 2021, 47(6): 1138-1148.

LI Wen-Lan, LI Wen-Cai, SUN Qi, YU Yan-Li, ZHAO Meng, LU Shou-Ping, LI Yan-Jiao, MENG Zhao-Dong. A study of expression pattern of auxin response factor family genes in maize (Zea mays L.)[J]. Acta Agronomica Sinica, 2021, 47(6): 1138-1148.

图/表 5

表1

实时定量PCR的引物序列"

基因名 Gene name	基因号 Gene accession	引物名称 Primer name	引物序列 Primer sequence (5°-3°)
ARF1	Zm00001	arf1-s	CGTGTATATGTATCCTTCC
ARF1	d030803	arf1-a	ATTGTCTTCTGAGTACCA
ARF2	Zm00001	arf2-s	AAACGACTCTGGGTATGT
ARF2	d031064	arf2-a	CTGAAGGACTTGTGTCTG
ARF3	Zm00001	arf3-s	AACGGCATCTCTAACTAC
ARF3	d031522	arf3-a	ACGAATCTATGGAATTGAAC
ARF4	Zm00001	arf4-s	AGTTCCGATGGCAGTGTT
ARF4	d032683	arf4-a	CGAGGAACCGATGCAGAT
ARF5	Zm00001	arf5-s	CGCAACAACAACAGGCATG
ARF5	d001879	arf5-a	GGGCTAAAAAGGGACTGGTT
ARF6	Zm00001	arf6-s	AATCTCAGCAGCAGTTAA
ARF6	d001945	arf6-a	CTTGGGACTCTTGGTTTA
ARF7	Zm00001	arf7-s	CTAGTGACGCCCTGTACC
ARF7	d003601	arf7-a	AGTAATAGACACGCTCGC
ARF8	Zm00001	arf8-s	GTAGTTGAAGTGGATAATTGTT
ARF8	d041056	arf8-a	TCTGGAGAAGGCTGATTA
ARF9	Zm00001	arf9-s	TCTGAACCTCTGGTATCC
ARF9	d041056	arf9-a	AACAATTATCCACTTCAACTAC
ARF10	Zm00001	arf10-s	TGAACTCGAAATCAGCTGC
ARF10	d042267	arf10-a	AACTCCAACCTCCACTTGC
ARF11	Zm00001d	arf11-s	ATGTTACAGGGAATGGGAATG
ARF11	043431	arf11-a	CAACAGTTTGAGGAGCAGACG
ARF12	Zm00001d	arf12-s	ATGGTAGACTTGATAGGA
ARF12	043922	arf12-a	CATATTCACAGTTCCAGTA
ARF13	Zm00001d	arf13-s	CAAGGCAATCACAATCTG
ARF13	049295	arf13-a	CTGTCTGTTCATCCCAAA
ARF14	Zm00001d	arf14-s	AATGACCGTTCTACTCCAATCA
ARF14	050781	arf14-a	CTATCTCAATGCCAAACAATCT
ARF15	Zm00001d	arf15-s	ATGAGGTCTTCGCCAGGAT
ARF15	051172	arf15-a	GGACTGTGTCAGCGTCTTG
ARF16	Zm00001d	arf16-s	AGAACATTGCTGATAGAT
ARF16	053819	arf16-a	TTGTGTATGACCTTGAAT
ARF17	Zm00001d	arf17-s	CCGTATATCCAAGGGTTTTG
ARF17	014013	arf17-a	ATGTGGGGTCTCTTTATGTCA
ARF18	Zm00001d	arf18-s	GCAGCAGATGGGGAAGCA
ARF18	014377	arf18-a	AACTCGACCGAACCGACG
ARF19	Zm00001d	arf19-s	AGAGGACGGCGGCAAGAT
ARF19	014507	arf19-a	TGCTCGCCCTCGGGTAGT
ARF20	Zm00001d	arf20-s	CCACCAATGAAGCAAGAA
ARF20	015243	arf20-a	GATAGACAACATCTGACACAT
ARF21	Zm00001d	arf21-s	AACAGAACAGCATTCAGT
ARF21	014690	arf21-a	TGATTCAGTGGAAGAGATG
ARF22	Zm00001d	arf22-s	GCTTTCCGCCAGCCTCA
ARF22	016838	arf22-a	CCGGTGTCACCACCGATG
基因名 Gene name	基因号 Gene accession	引物名称 Primer name	引物序列 Primer sequence (5°-3°)
ARF23	Zm00001d	arf23-s	CTGAGAGGACGGTGAGCAA
ARF23	000358	arf23-a	CGCGACAGCCGAGAGGT
ARF24	Zm00001d	arf24-s	GCCACTTTCAAGTCAGATT
ARF24	036593	arf24-a	TTGGATTGTGCTCTCAGA
ARF25	Zm00001d	arf25-s	CCCTCTTCTGTTCTTATGTTT
ARF25	038698	arf25-a	TACTTCTTCACGGTTGGT
ARF26	Zm00001d	arf26-s	GCATTCGCCCTCTTCTGTT
ARF26	038698	arf26-a	AGGCTCGCTTCCATTTACA
ARF27	Zm00001d	arf27-s	TTCCATCTCAATCCTCAT
ARF27	039006	arf27-a	TCTATCCTCTTTCTTATTCAC
ARF28	GRMZM2	arf28-s	TTATGGTTCCAATACAAGAA
ARF28	G075715	arf28-a	CTCATTCCTATTCCTTAGC
ARF29	Zm00001d	arf29-s	TTCAAGATCAGGGTTCAG
ARF29	011953	arf29-a	GATTCAACCGTCAGAGAA
ARF30	Zm00001d	arf30-s	CAAGTTCTTCAACATCAG
ARF30	045026	arf30-a	ATCCTGTATTATGGTTCAA
ARF31	Zm00001d	arf31-s	ACTCGCTGGGAAGAGGGCT
ARF31	023659	arf31-a	CCTTTTGTCTGCTTCACCAC
ARF32	Zm00001d	arf32-s	AGCTGGTGCGGGGCAAC
ARF32	025871	arf32-a	CCTGCAAGGCCTCAATGAC
ARF33	Zm00001d	arf33-s	AGTTGAATGCTCTTGGTA
ARF33	026540	arf33-a	GTGAATCTGTGCTTCTTG
ARF34	Zm00001d	arf34-s	ATGCTGGGTTGTTTGGTT
ARF34	026590	arf34-a	GCGGCTAGAAAGTGGAAT
ARF35	Zm00001d	arf35-s	ATGATATTGGAGCAGATG
ARF35	026687	arf35-a	AAGAGCATTATGGTGTTC
18S rRNA		18S-s	AAACGGCTACCACATCCAAG
18S rRNA		18S-a	CCTCCAATGGATCCTCGTTA

表1

图1

图2

表2

玉米ARF家族基因启动子区的顺式作用元件"

基因 Gene	元件A Element A	元件B Element B	元件C Element C
ARF1	ABRE¹, AuxRR-core¹, CGTCA-motif², P-box², TGA-element², TGACG-motif²		MBS¹
ARF2	ABRE³, CGTCA-motif³, P-box¹, TGACG-motif³	CAT-box²
ARF3	ABRE³, CGTCA-motif⁴, GARE-motif¹, P-box¹, TGACG-motif⁴	CAT-box²	TCA-element², LTR¹
ARF4	ABRE¹, CGTCA-motif¹, P-box¹, TGACG-motif¹	GCN4_motif¹	LTR¹, MBS¹
ARF5	ABRE¹, CGTCA-motif¹, GARE-motif¹, TGA-element¹, TGACG-motif¹	CAT-box³	TCA-element¹, LTR²
ARF6	ABRE², CGTCA-motif¹, TGA-element¹, TGACG-motif¹	CAT-box², RY-element¹	MBS¹
ARF7	ABRE², CGTCA-motif¹, TGA-element¹, TGACG-motif¹	CAT-box¹	LTR³, MBS¹
ARF8	ABRE², CGTCA-motif³, GARE-motif¹, TGACG-motif³		LTR¹, MBS¹, TC-rich repeats¹
ARF9	ABRE², CGTCA-motif³, GARE-motif¹, TGACG-motif³		LTR¹, MBS¹, TC-rich repeats¹
ARF10	ABRE¹, CGTCA-motif⁵, TGACG-motif⁵
ARF11	ABRE², CGTCA-motif², TGA-element¹, TGACG-motif²	CAT-box²	TCA-element¹
ARF12	ABRE¹, CGTCA-motif¹, TGACG-motif¹	CAT-box¹	MBS²
ARF13	ABRE², CGTCA-motif², P-box¹, TGACG-motif²		TC-rich repeats¹
ARF14	ABRE², TGA-element¹		TCA-element²
ARF15	CGTCA-motif³, TGA-element¹, TGACG-motif³		LTR¹, MBS¹
ARF16	ABRE¹, AuxRR-core¹, CGTCA-motif², TGACG-motif²	CAT-box³
ARF17	ABRE¹, CGTCA-motif⁴, TGA-element¹, TGACG-motif⁴	RY-element¹	MBS¹, TCA-element¹
ARF18	ABRE², CGTCA-motif¹, P-box¹, TGACG-motif¹		MBS¹
ARF19	P-box¹	CAT-box¹	LTR¹, MBS¹, TCA-element¹
ARF20	ABRE³, CGTCA-motif¹, TGA-element¹, TGACG-motif¹		LTR¹, TCA-element¹
ARF21	ABRE⁷, CGTCA-motif², TGACG-motif²		MBS¹
ARF22	ABRE⁶, CGTCA-motif², GARE-motif¹, TGACG-motif²	CAT-box¹
ARF23	ABRE⁴, AuxRR-core¹, CGTCA-motif¹, TGA-element², TGACG-motif¹	CAT-box¹	LTR¹, MBS², TCA-element¹
ARF24	ABRE³, CGTCA-motif², GARE-motif¹, P-box², TGACG-motif²		MBS¹, TCA-element¹
ARF25	ABRE¹, CGTCA-motif¹, P-box¹, TGACG-motif¹	CAT-box³	LTR¹, TC-rich repeats¹
ARF26	ABRE¹, CGTCA-motif¹, P-box¹, TGACG-motif¹	CAT-box³	LTR¹, TC-rich repeats¹
ARF27	ABRE⁴, GARE-motif¹	RY-element¹
ARF28	ABRE¹, CGTCA-motif², GARE-motif¹, TGACG-motif²	CAT-box²	MBS², TCA-element²
ARF29	ABRE³, AuxRR-core¹, CGTCA-motif¹, TGACG-motif¹	CAT-box¹	LTR¹
ARF30	ABRE⁶, CGTCA-motif⁴, TGACG-motif⁴	circadian¹	MBS², TC-rich repeats¹
ARF31	ABRE¹, CGTCA-motif², TGACG-motif²	circadian¹	LTR²
ARF32	ABRE⁵, CGTCA-motif¹, TGACG-motif¹	CAT-box¹, GCN4_motif¹, RY-element³	TCA-element¹
ARF33	ABRE⁴, CGTCA-motif³, GARE-motif¹, TGA-element¹, TGACG-motif³	CAT-box⁴, RY-element¹
ARF34	ABRE¹, GARE-motif¹, TGA-element¹	CAT-box²	LTR¹
ARF35	ABRE³		MBS¹

表2

图3

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