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作物学报 ›› 2022, Vol. 48 ›› Issue (1): 48-62.doi: 10.3724/SP.J.1006.2022.11006
所属专题： 小麦：遗传育种·种质资源·分子遗传学
李玲红(
), 张哲, 陈永明, 尤明山, 倪中福, 邢界文*()
LI Ling-Hong(), ZHANG Zhe, CHEN Yong-Ming, YOU Ming-Shan, NI Zhong-Fu, XING Jie-Wen*()
摘要：
为了明确突变体颖壳蜡质含量显著变化的分子机制, 本研究对源自济麦22颖壳蜡质缺失突变体glossy1与野生型进行了转录组分析。结果表明, 在glossy1突变体中, 共筛选到12,230个差异表达基因, 其中5811个基因在突变体中上调表达, 6419个下调表达。GO (gene ontology)功能富集分析发现, 差异基因主要富集在蜡质合成和转运途径, 具体分布在酰基转移酶活性、脂质结合和水解酶活性等条目, 由此推测这些途径与小麦穗部蜡质缺失性状是紧密相关的。我们还利用RT-qPCR检测了参与蜡质代谢途径部分基因的表达, 结果与转录组结果是一致的。本研究为今后探究小麦蜡质代谢的分子机制和基因调控网络提供了数据支持, 同时也为抗逆小麦育种奠定了理论基础。
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