小麦卷叶突变体RL1的生理特性及遗传研究

doi:10.3724/SP.J.1006.2023.31004

作物学报 ›› 2023, Vol. 49 ›› Issue (12): 3399-3410.doi: 10.3724/SP.J.1006.2023.31004

• 研究简报 • 上一篇

小麦卷叶突变体RL1的生理特性及遗传研究

刘叶¹^,²(), 李越¹, 苑名杨¹, 卫乃翠¹, 关攀锋³, 赵佳佳¹, 武棒棒¹, 郑兴卫¹, 郝宇琼¹, 乔玲¹^,^*(), 郑军¹^,^*()

¹山西农业大学小麦研究所 / 农业农村部有机旱作农业重点实验室(部省共建), 山西临汾 041000
²山西大学生命科学学院, 山西太原 030006
³郑州大学农学院, 河南郑州 450001

收稿日期:2023-01-09 接受日期:2023-06-29 出版日期:2023-12-12 网络出版日期:2023-08-04
通讯作者: * 乔玲, E-mail: qiaolingsmile@163.com; 郑军, E-mail: sxnkyzj@126.com
作者简介:刘叶, E-mail: liuye12345202@126.com^**同等贡献
基金资助:
山西农业大学省部共建有机旱作农业国家重点实验室自主研发项目(202002-1);山西省科技重大专项计划“揭榜挂帅”项目(202201140601025-2-01)

Physiological characteristics and genetic research of rolled leaf mutant1 (RL1) in wheat (Triticum aestivum L.)

LIU Ye¹^,²(), LI Yue¹, YUAN Ming-Yang¹, WEI Nai-Cui¹, GUAN Pan-Feng³, ZHAO Jia-Jia¹, WU Bang-Bang¹, ZHENG Xing-Wei¹, HAO Yu-Qiong¹, QIAO Ling¹^,^*(), ZHENG Jun¹^,^*()

¹Institute of Wheat Research, Shanxi Agriculture University / Key Laboratory of Sustainable Dryland Agriculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Linfen 041000, Shanxi, China
²School of Life Science, Shanxi University, Taiyuan 030006, Shanxi, China
³School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, Henan, China

Received:2023-01-09 Accepted:2023-06-29 Published:2023-12-12 Published online:2023-08-04
Contact: * E-mail: qiaolingsmile@163.com; E-mail: sxnkyzj@126.com
About author:^**Contributed equally to this work
Supported by:
Research Program Sponsored by State Key Laboratory of Integrative Sustainable Dryland Agriculture, Shanxi Agricultural University(202002-1);Science and Technology Major Special Plan Project “Reveal the Title” of Shanxi Province(202201140601025-2-01)

摘要/Abstract

摘要：

小麦叶片在逆境下会发生可逆的折叠或卷曲, 通过脱水回避的形态学变化降低非生物胁迫的损害。目前小麦叶片卷曲的生理和遗传调控机制尚不清楚。本文利用EMS诱变晋麦47获得了卷叶突变体RL1 (Rolled Leaf 1), RL1在整个生育期叶片呈现卷曲, 初生叶片沿中轴脉向近轴面微卷, 随着叶片生长加速卷曲, 直至为筒状。与野生型相比, RL1株高降低、穗长变短、旗叶变窄和千粒重降低。氯化三苯基四氮唑(TTC)染色结果表明RL1种子活力低, 且种子发芽率降低了22%。抽穗10 d后, RL1的叶绿素含量与野生型基本一致, 净光合速率、蒸腾速率、气孔导度、细胞间隙CO₂浓度差异不显著, 但水分利用率降低。低温、高温和干旱促进RL1的叶片卷曲; 石蜡切片观察表明, RL1的大叶脉和小叶脉偏少, 在中脉区域远轴面厚壁细胞和近轴面薄壁细胞数目减少, 且维管束间泡状细胞的面积和数量均明显低于野生型; RL1叶片不同部位泡状细胞缩小以及维管束减少导致整个叶片向近轴面极度卷曲。遗传分析表明该性状受1对不完全显性的核基因控制, 位于1D染色体短臂上, 精细定位将目标区间锁定在9.42 Mb范围内。

关键词: 小麦, 卷叶突变体, 细胞学分析, 生理特性, 遗传分析

Abstract:

Wheat leaves tend to fold or curl when exposure to stresses, the dehydration avoidance in morphology can reduce the damage of abiotic stress. At present, the physiological and genetic regulation mechanism associated with leaf curling is not clear in wheat. This study reported a novel rolled leaf mutant (RL1) from the ethyl methyl sulphonate (EMS) mutagenesis cultivar Jinmai 47. The leaves of the mutant RL1 were curled during the growth period, and the primary leaves were slightly curled along axial vein to paraxial plane. Leaf curling was accelerated with the growth until the leaf was tubular. Compared to wild type (WT), plant height, ear length, flag leaf narrowing, and 1000-grain weight were decreased in mutant RL1. Triphenyltetrazolium Chloride (TTC) staining showed that seed vigor of RL1 was low, together with the decreased germination rate by 22%. Additionally, there was no significant differences in chlorophyll content, net photosynthetic rate, transpiration rate, stomatal conductance, and intercellular CO₂ concentration between RL1 and WT, while water utilization rate was decreased in RL1 after heading for 10 days. Low temperature, high temperature, and drought led to the leaf rolling in RL1. RL1 showed fewer leaf/lobular veins via paraffin section assay, and the number of abaxial sclerenchyma and adaxial parenchyma cells were reduced in midrib region of RL1. Moreover, the area and counts of vesicular cells between the vascular bundles were significantly reduced in RL1, together with the vesicular cells at the midvein region of leaves compared with WT. Vesicular cells and vascular bundles shrunk and decreased, respectively, resulting in the situation that the entire blade was extremely crimped to the adaxial plane. Genetic analysis demonstrated that the mutant trait was localized on the short arm of chromosome 1D, regulated by a pair of nuclear genes with incomplete dominance and fine mapping analysis further locked the target interval at 9.42 Mb.

Key words: wheat, rolled leaf mutant, cytological analysis, physiological characteristics, genetic analysis

刘叶, 李越, 苑名杨, 卫乃翠, 关攀锋, 赵佳佳, 武棒棒, 郑兴卫, 郝宇琼, 乔玲, 郑军. 小麦卷叶突变体RL1的生理特性及遗传研究[J]. 作物学报, 2023, 49(12): 3399-3410.

LIU Ye, LI Yue, YUAN Ming-Yang, WEI Nai-Cui, GUAN Pan-Feng, ZHAO Jia-Jia, WU Bang-Bang, ZHENG Xing-Wei, HAO Yu-Qiong, QIAO Ling, ZHENG Jun. Physiological characteristics and genetic research of rolled leaf mutant1 (RL1) in wheat (Triticum aestivum L.)[J]. Acta Agronomica Sinica, 2023, 49(12): 3399-3410.

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://zwxb.chinacrops.org/CN/10.3724/SP.J.1006.2023.31004

https://zwxb.chinacrops.org/CN/Y2023/V49/I12/3399

图/表 12

表1

附表1

42对SSR引物信息(国标: NY/T 2859-2015)"

引物名称 Primer name	染色体 Chr.	退火温度 Annealing temperature (℃)	引物序列 Primer sequence (5'-3')
cwm65	1A	65	F: TCATTGGTGTCATCCCTCGTGT; R: GAATAATGCCTTGACCCTGGAC
barc80	1BL	65	F: GCGAATTAGCATCTGCATCTGTTTGAG; R: CGGTCAACCAACTACTGCACAAC
cfd72	1DL	60	F: CTCCTTGGAATCTCACCGAA; R: TCCTTGGGAATATGCCTCCT
gwm294	2AL	55	F: GGATTGGAGTTAAGAGAGAACCG; R: GCAGAGTGATCAATGCCAGA
gwm429	2BS	55	F: TTGTACATTAAGTTCCCATTA; R: TTTAAGGACCTACATGACAC
gwm261	2DS	55	F: CTCCCTGTACGCCTAAGGC; R: CTCGCGCTACTAGCCATTG
gwm155	3AL	55	F: CAATCATTTCCCCCTCCC; R: AATCATTGGAAATCCATATGCC
gwm285	3BS	65	F: ATGACCCTTCTGCCAAACAC; R: ATCGACCGGGATCTAGCC
gdm72	3DS	55	F: TGGTTTTCTCGAGCATTCAA; R: TGCAACGATGAAGACCAGAA
gwm610	4AS	65	F: CTGCCTTCTCCATGGTTTGT; R: AATGGCCAAAGGTTATGAAGG
ksum62	4B	60	F: GGAGAGGATAGGCACAGGAC; R: GAGAGCAGAGGGAGCTATGG
barc91	4DL	55	F: TTCCCATAACGCCGATAGTA; R: GCGTTTAATATTAGCTTCAAGATCAT
gwm304	5AS	55	F: AGGAAACAGAAATATCGCGG; R: AGGACTGTGGGGAATGAATG
gwm67	5BL	60	F: ACCACACAAACAAGGTAAGCG; R: CAACCCTCTTAATTTTGTTGGG
cfd29	5DL	65	F: GGTTGTCAGGCAGGATATTTG; R: TATTGATAGATCAGGGCGCA
gwm459	6AS	55	F: AATTTCAAAAAGGAGAGAGA; R: AACATGTGTTTTTAGCTATC
barc198	6BS	55	F: CGCTGAAAAGAAGTGCCGCATTATGA; R: CGCTGCCTTTTCTGGATTGCTTGTCA
cfd76	6DL	65	F: GCAATTTCACACGCGACTTA; R: CGCTCGACAACATGACACTT
cfa2028	7AS	55	F: TGGGTATGAAAGGCTGAAGG; R: ATCGCGACTATTCAACGCTT
gwm333	7BS	60	F: GCCCGGTCATGTAAAACG; R: TTTCAGTTTGCGTTAAGCTTTG
gwmn437	7DL	55	F: GATCAAGACTTTTGTATCTCTC; R: GATGTCCAACAGTTAGCTTA
wmc312	1AS	55	F: TGTGCCCGCTGGTGCGAAG; R: CCGACGCAGGTGAGCGAAG
barc240	1BL	55	F: AGAGGACGCTGAGAACTTTAGAGAA; R: GCGATCTTTGTAATGCATGGTGAAC
gdm111	1DL	55	F: CACTCACCCCAAACCAAAGT; R: GATGCAATCGGGTCGTTAGT
wmc522	2AS	55	F: AAAAATCTCACGAGTOGGGC; R: CCCGAGCAGGAGCTACAAAT
cfd51	2DS	55	F: GGAGGCTTCTCTATGGGAGG; R: TGCATCTTATCCTGTGCAGC
barc324	3AS	55	F: CCAATTCTGCCCATAGGTGA; R: GAGGAAATAAGATTCAGCCAACTG
barc164	3BS	55	F: TGCAAACTAATCACCAGCGTAA; R: CGCTTTCTAAAACTGTTCGGGATTTCTAA
cfd9	3DL	55	F: TTGCACGCACCTAAACTCTG; R: CAAGTGTGAGCGTCGG
gwm161	3DS	55	F: GATCGAGTGATGGCAGATGG; R: TGTGAATTACTTGGACGTGG
barc170	4AL	55	F: CGCTTGACTTTGAATGGCTGAACA; R: CGCCCACTTTTTACCTAATCCTTTTGAA
gwm495	4BL	55	F: GAGAGCCTCGCGAAATATAGG; R: TGCTTCTGGTGTTCCTTCG
winc720	4DS	55	F: CACCATGGTTGGCAAGAGA; R: CTGGTGATACTGCCGTGACA
gwm186	5AL	55	F: GCAGAGCCTGGTTCAAAAAG; R: CGCCTCTAGCGAGAGCTATG
cfa2155	5AL	55	F: TTTGTTACAACCCAGGGGG; R: TTGTGTGGCGAAAGAAACAG
cfd8	5DS	60	F: ACCACCGTCATGTCACTGAG; R: GTGAAGACGACAAGACGCAA
gwml69	6AL	55	F: ACCACTGCAGAGAACACATACG; R: GTGCTCTGCTCTAAGTGTGGG
barc345	6BL	55	F: CGCCAGACTGCTAGGATAATACTTT; R: GCGGCTAGTGCTCCCTCATAAT
barcl121	6DL	60	F: GCGAGCAAACTGATCCCAAAAAG; R: TATCGGTGAGTACGCCAAAAACA
cfa2123	7AS	60	F: CGGTCTTTGTTTGCTCTAAACC; R: ACCGGCCATCTATGATGAAG
wmc476	7BS	55	F: TACCAACCACACCTGCGAGT; R: CTAGATGAACCTTCGTGCGG
gwm44	7DS	65	F: GTTGAGCTTTTCAGTTCGGC; R: ACTGGCATCCACTGAGCTG

附表1

图1

图2

表2

图3

图4

图5

附表2

表3

图6

表4

水稻叶片卷曲相关基因在小麦中的同源基因"

水稻基因 Rice gene	基因注释 Gene annotation	部分同源群 Homoeologous groups	小麦的同源基因 Wheat gene
OsACL1	Os04g33860	2	TraesCS2A01G296600 TraesCS2B01G312800 TraesCS2D01G294500
OsDEK1	Os02g47970	6	TraesCS6A01G275500 TraesCS6B01G302900 TraesCS6D01G255800
OsCFL1	Os02g31140	4	TraesCS4A01G407300 TraesCS4B01G306500 TraesCS4D01G304700
OsMYB103L	Os08g05520	7	TraesCS7A01G304500 TraesCS7B01G204800 TraesCS7D01G300000
OsVIL3	Os02g05840	6	TraesCS6A02G123400 TraesCS6B02G151600 TraesCS6D02G113600
OsAGO7	Os03g33650	2	TraesCS2A01G414800 TraesCS2B01G434000 TraesCS2D01G412100
OsCOW1	Os03g06654	4	TraesCS4A01G027500 TraesCS4B01G278300 TraesCS4D01G276600
OsCSLD4	Os12g36890	5	TraesCS5A01G090500 TraesCS5B01G096200 TraesCS5D01G102600
OsHOX32	Os03g43930	5	TraesCS5A01G375800 TraesCS5B01G378000 TraesCS5D01G385300
OsYAB1	Os07g06620	5	TraesCS5A01G371500 TraesCS5B01G373600 TraesCS5D01G380900
OsZHD1	Os09g29130	5	TraesCS5A01G246500 TraesCS5B01G243800 TraesCS5D01G253100
OsPSL1	Os01g19170	1	TraesCS1A01G311100 TraesCS1B01G322500 TraesCS1D01G311000
OsREL1	Os01g64380	2	TraesCS2A01G065100 TraesCS2B01G077300 TraesCS2D01G063200
OsREL2	Os10g41310	1	TraesCS1A01G218200 TraesCS1B01G231600 TraesCS1D01G219800
Osrl14	Os10g40960	1	TraesCS1A01G221200 TraesCS1B01G234500 TraesCS1D01G222800
OsROC5	Os02g45250	6	TraesCS6A01G255800 TraesCS6B01G269700 TraesCS6D01G237000
OsRoc8	Os06g10600	6	TraesCS6A01G324500 TraesCS6B01G354900 TraesCS6D01G304300
OsSLL1	Os09g23200	5	TraesCS5A01G203200 TraesCS5B01G201900 TraesCS5D01G209600
OsSRL2	Os03g19520	4	TraesCS4A01G121600 TraesCS4B01G181700 TraesCS4D01G184300
OsSrl1	Os07g01240	2	TraesCS2A01G256400 TraesCS2B01G286100 TraesCS2D01G347200
OsOFP1	Os01g12690	3	TraesCS3A01G151800 TraesCS3B01G178800 TraesCS3D01G159700
OsMKB3	Os03g52320	4	TraesCS4A01G250600 TraesCS4B01G064000 TraesCS4D01G062900
OsLBD3-7	Os03g57670	5	TraesCS5A01G515300 TraesCS5B01G282700 TraesCS5D01G291300
OsARVL4	Os04g33580	1	TraesCS1A01G036100 TraesCS1B01G231600 TraesCS1D01G219800
OsARF18	Os06g47150	7	TraesCS7A01G446900 TraesCS7B01G346700 TraesCS7D01G436800
OsWOX3A	Os12g01120	5	TraesCS5A01G157300 TraesCS5B01G156400 TraesCS5D01G162600

表4

参考文献 46

[1]	Bogard M, Hourcade D, Piquemal B, Gouache D, Deswartes J C, Throude M, Cohan J P. Marker-based crop model-assisted ideotype design to improve avoidance of abiotic stress in bread wheat. J Exp Bot, 2021, 72: 1085-1103. doi: 10.1093/jxb/eraa477 pmid: 33068400
[2]	Merrium S, Ali Z, Tahir M H N, Habib-Ur-Rahman M, Hakeem S. Leaf rolling dynamics for atmospheric moisture harvesting in wheat plant as an adaptation to arid environments. Environ Sci Pollut Res Int, 2022, 29: 48995-49006. doi: 10.1007/s11356-022-18936-2
[3]	Sirault X R R, Condon A G, Wood J T, Farquhar G D, Rebetzke G J. ‘Rolled-upness’: phenotyping leaf rolling in cereals using computer vision and functional data analysis approaches. Plant Methods, 2015, 11: 52. doi: 10.1186/s13007-015-0095-1 pmid: 26583042
[4]	Zhang X Y, Jia H Y, Li T, Wu J Z, Nagarajan R, Lei L, Powers C, Kan C C, Hua W, Liu Z Y, Chen C, Carver B F, Yan L L. TaCol-B5 modifies spike architecture and enhances grain yield in wheat. Science, 2022, 376: 180-183. doi: 10.1126/science.abm0717
[5]	Sun J, Cui X A, Teng S Z, Zhao K N, Wang Y W, Chen Z H, Sun X H, Wu J X, Ai P F, Quick W P, Lu T G, Zhang Z G. HD-ZIP IV gene Roc8 regulates the size of bulliform cells and lignin content in rice. Plant Biotechnol J, 2020, 18: 2559-2572. doi: 10.1111/pbi.v18.12
[6]	Li L, Shi Z Y, Li L, Shen G Z, Wang X Q, An L S, Zhang J L. Overexpression of ACL1 (abaxially curled leaf 1) increased bulliform cells and induced abaxial curling of leaf blades in rice. Mol Plant, 2010, 3: 807-817. doi: 10.1093/mp/ssq022
[7]	Zou L P, Sun X H, Zhang Z G, Liu P, Wu J X, Tian C J, Qiu J L, Lu T G. Leaf rolling controlled by the homeodomain leucine zipper class IV gene Roc5 in rice. Plant Physiol, 2011, 156: 1589-1602. doi: 10.1104/pp.111.176016
[8]	Zhang G H, Xu Q, Zhu X D, Qian Q, Xue H W. SHALLOT- LIKE1 is a KANADI transcription factor that modulates rice leaf rolling by regulating leaf abaxial cell development. Plant Cell, 2009, 21: 719-735. doi: 10.1105/tpc.108.061457
[9]	邹良平. 水稻卷叶突变体的细胞形成机制以及OUL1基因的克隆和功能研究. 中国农业科学院博士学位论文,北京, 2012.
	Zou L P. Cytological Mechanism of Rolled-feaf Formation and Functional Analysis of OUL1 Controlling Leaf Roll in Rice. PhD Dissertation of Chinese Academy of Agricultural Sciences, Beijing, China, 2012. (in Chinese with English abstract)
[10]	Wu R H, Li S B, He S, Wassmann F, Yu C H, Qin G J, Schreiber L, Qu L J, Gu H Y. CFL1, a WW domain protein, regulates cuticle development by modulating the function of HDG1, a class IV homeodomain transcription factor, in rice and Arabidopsis. Plant Cell, 2011, 23: 3392-3411. doi: 10.1105/tpc.111.088625
[11]	Juarez M T, Twigg R W, Timmermans M C P. Specification of adaxial cell fate during maize leaf development. Development, 2004, 131: 4533-4544. doi: 10.1242/dev.01328 pmid: 15342478
[12]	Canales C, Grigg S, Tsiantis M. The formation and patterning of leaves: recent advances. Planta, 2005, 221: 752-756. pmid: 15909148
[13]	Zhu Z, Wang J Y, Li C N, Li L, Mao X G, Hu G, Wang J P, Chang J Z, Jing R L. A transcription factor TaMYB5 modulates leaf rolling in wheat. Front Plant Sci, 2022, 13: 897623. doi: 10.3389/fpls.2022.897623
[14]	Verma A, Niranjana M, Jha S K, Mallick N, Agarwal P, Vinod. QTL detection and putative candidate gene prediction for leaf rolling under moisture stress condition in wheat. Sci Rep, 2020, 10: 18696. doi: 10.1038/s41598-020-75703-4 pmid: 33122772
[15]	Yang X, Wang J Y, Mao X G, Li C N, Li L, Xue Y H, He L H, Jing R L. A locus controlling leaf rolling degree in wheat under drought stress identified by bulked segregant analysis. Plants, 2022, 11: 2076. doi: 10.3390/plants11162076
[16]	Bian R L, Liu N, Xu Y Z, Su Z Q, Chai L L, Bernardo A, Amand P St, Fritz A, Zhang G R, Rupp J, Akhunov E, Jordan K W, Bai G H. Quantitative trait loci for rolled leaf in a wheat EMS mutant from Jagger. Theor Appl Genet, 2023, 136: 52. doi: 10.1007/s00122-023-04284-3
[17]	赵佳佳, 乔玲, 武棒棒, 葛川, 乔麟轶, 张树伟, 闫素仙, 郑兴卫, 郑军. 山西省小麦苗期根系性状及抗旱特性分析. 作物学报, 2021, 47: 714-727. doi: 10.3724/SP.J.1006.2021.01048
	Zhao J J, Qiao L, Wu B B, Ge C, Qiao L Y, Zhang S W, Yan S X, Zheng X W, Zheng J. Seedling root characteristics and drought resistance of wheat in Shanxi province. Acta Agron Sin, 2021, 47: 714-727. (in Chinese with English abstract) doi: 10.3724/SP.J.1006.2021.01048
[18]	李浩然, 李慧玲, 王红光, 李东晓, 李瑞奇, 李雁鸣. 冬小麦叶面积测算方法的再探讨. 麦类作物学报, 2018, 38: 455-459.
	Li H R, Li H L, Wang H G, Li X D, Li R Q, Li Y M. Further study on the method of leaf area calculation in winter wheat. J Triticeae Crops, 2018, 38: 455-459. (in Chinese with English abstract)
[19]	Shi Z Y, Wang J, Wan X S, Shen G Z, Wang X Q, Zhang J L. Over-expression of rice OsAGO7 gene induces upward curling of the leaf blade that enhanced erect-leaf habit. Planta, 2007, 226: 99-108. doi: 10.1007/s00425-006-0472-0
[20]	Selim D A H, Zayed M, Ali M M E, Eldesouky H S, Bonfill M, El-Tahan A M, Ibrahim O M, El-Saadony M T, El-Tarabily K A, AbuQamar S F, Elokkiah S. Germination, physio-anatomical behavior, and productivity of wheat plants irrigated with magnetically treated seawater. Front Plant Sci, 2022, 13: 923872. doi: 10.3389/fpls.2022.923872
[21]	张礼霞, 刘合芹, 于新, 王林友, 范宏环, 金庆生, 王建军. 水稻卷叶突变体rl15(t)的生理学分析及基因定位. 中国农业科学, 2014, 47: 2881-2888. doi: 10.3864/j.issn.0578-1752.2014.14.018
	Zhang L X, Liu H Q, Yu X, Wang L Y, Fan H H, Jin Q S, Wang J J. Molecular mapping and physiological characterization of a novel mutant rl15(t) in rice. Sci Agric Sin, 2014, 47: 2881-2888. (in Chinese with English abstract) doi: 10.3864/j.issn.0578-1752.2014.14.018
[22]	严长杰, 严松, 张正球, 梁国华, 陆驹飞, 顾铭洪. 一个新的水稻卷叶突变体rl9(t)的遗传分析和基因定位. 科学通报, 2005, 50: 2757-2762.
	Yan C J, Yan S, Zhang Z Q, Liang G H, Lu J F, Gu M H. Genetic analysis and gene fine mapping for a rice novel mutant rl9(t) with rolling leaf character. Sci Bull, 2005, 50: 2757-2762. (in Chinese with English abstract)
[23]	Duan P G, Ni S, Wang J M, Zhang B L, Xu R, Wang Y X, Chen H Q, Zhu X D, Li Y H. Regulation of OsGRF4 by OsmiR396 controls grain size and yield in rice. Nat Plants, 2015, 2: 15203. doi: 10.1038/nplants.2015.203
[24]	Li Y Y, Shen A, Xiong W, Sun Q L, Luo Q, Song T, Li Z L, Luan W J. Overexpression of OsHox32 results in pleiotropic effects on plant type architecture and leaf development in rice. Rice, 2016, 9: 46. doi: 10.1186/s12284-016-0118-1
[25]	Liu X F, Li M, Liu K, Tang D, Sun M F, Li Y F, Shen Y, Du G J, Cheng Z K. Semi-Rolled Leaf2 modulates rice leaf rolling by regulating abaxial side cell differentiation. J Exp Bot, 2016, 67: 2139-2150. doi: 10.1093/jxb/erw029
[26]	Shimano S, Hibara K I, Furuya T, Arimura S I, Tsukaya H, Itoh J I. Conserved functional control, but distinct regulation, of cell proliferation in rice and Arabidopsis leaves revealed by comparative analysis of GRF-INTERACTING FACTOR 1 orthologs. Development, 2018, 145: 159624.
[27]	Jane W N, Chiang S H T. Morphology and development of bulliform cells in Arundo formosana Hack. Taiwania Int J Life Sci, 1991, 36: 85-97.
[28]	Hibara K L, Obara M, Hayashida E, Abe M, Ishimaru T, Satoh H, Itoh J L, Nagato Y. The ADAXIALIZED LEAF1 gene functions in leaf and embryonic pattern formation in rice. Dev Biol, 2009, 334: 345-354. doi: 10.1016/j.ydbio.2009.07.042
[29]	Xu Y, Wang Y H, Long Q Z, Huang J X, Wang Y L, Zhou K N, Zheng M, Sun J, Chen H, Chen S H, Jiang L, Wang C M, Wan J M. Overexpression of OsZHD1, a zinc finger homeodomain class homeobox transcription factor, induces abaxially curled and drooping leaf in rice. Planta, 2014, 239: 803-816. doi: 10.1007/s00425-013-2009-7
[30]	Li C, Zou X H, Zhang C Y, Shao Q H, Liu J, Liu B, Li H Y, Zhao T. OsLBD3-7 overexpression induced adaxially rolled leaves in rice. PLoS One, 2016, 11: e0156413. doi: 10.1371/journal.pone.0156413
[31]	Yang C H, Li D Y, Liu X, Ji C J, Hao L L, Zhao X F, Li X B, Chen C Y, Cheng Z K, Zhu L H. OsMYB103L, an R2R3-MYB transcription factor, influences leaf rolling and mechanical strength in rice (Oryza sativa L.). BMC Plant Biol, 2014, 14: 158. doi: 10.1186/1471-2229-14-158
[32]	Kinoshita T. Gene analysis and linkage map. Tokyo: Japan Scientific Societies Press, 1984. pp 187-274.
[33]	Khush G S, Kinoshita T. Rice karyotype, marker genes, and linkage groups. In: Khush G S, Toenniessen G H, eds. Rice Biology. Wallingford: CAB International and International Rice Research Institute, 1991. pp 83-108.
[34]	Wang J, Hu J, Qian Q, Xue H W. LC2 and OsVIL2 promote rice flowering by photoperoid-induced epigenetic silencing of OsLF. Mol Plant, 2013, 6: 514-527. doi: 10.1093/mp/sss096 pmid: 22973062
[35]	Woo Y M, Park H J, Su'udi M, Yang J I, Park J J, Back K, Park Y M, An G. Constitutively wilted 1, a member of the rice YUCCA gene family, is required for maintaining water homeostasis and an appropriate root to shoot ratio. Plant Mol Biol, 2007, 65: 125-136. doi: 10.1007/s11103-007-9203-6
[36]	Hu J, Zhu L, Zeng D L, Gao Z Y, Guo L B, Fang Y X, Zhang G Z, Dong G J, Yan M X, Liu J, Qian Q. Identification and characterization of NARROW AND ROLLED LEAF 1, a novel gene regulating leaf morphology and plant architecture in rice. Plant Mol Biol, 2010, 73: 283-292. doi: 10.1007/s11103-010-9614-7
[37]	Dai M Q, Zhao Y, Ma Q, Hu Y F, Hedden P, Zhang Q F, Zhou D X. The rice YABBY1 gene is involved in the feedback regulation of gibberellin metabolism. Plant Physiol, 2007, 144: 121-133.
[38]	Zhang G H, Hou X, Wang L, Xu J, Chen J, Fu X, Shen N W, Nian J Q, Jiang Z Z, Hu J, Zhu L, Rao Y C, Shi Y F, Ren D Y, Dong G J, Gao Z Y, Guo L B, Qian Q, Luan S. PHOTO- SENSITIVE LEAF ROLLING 1 encodes a polygalacturonase that modifies cell wall structure and drought tolerance in rice. New Phytol, 2021, 229: 890-901. doi: 10.1111/nph.v229.2
[39]	Chen Q L, Xie Q J, Gao J, Wang W Y, Sun B, Liu B H, Zhu H T, Peng H F, Zhao H B, Liu C H, Wang J, Zhang J L, Zhang G Q, Zhang Z M. Characterization of Rolled and Erect Leaf 1 in regulating leave morphology in rice. J Exp Bot, 2015, 66: 6047-6058. doi: 10.1093/jxb/erv319
[40]	Yang S Q, Li W Q, Miao H, Gan P F, Qiao L, Chang Y L, Shi C H, Chen K M. REL2, a gene encoding an unknown function protein which contains DUF630 and DUF632 domains controls leaf rolling in rice. Rice, 2016, 9: 37. doi: 10.1186/s12284-016-0105-6
[41]	Fang L K, Zhao F M, Cong Y F, Sang X C, Du Q, Wang D Z, Li Y F, Ling Y H, Yang Z L, He G H. Rolling-leaf14 is a 2OG-Fe (II) oxygenase family protein that modulates rice leaf rolling by affecting secondary cell wall formation in leaves. Plant Biotechnol J, 2012, 10: 524-532. doi: 10.1111/pbi.2012.10.issue-5
[42]	Xiang J J, Zhang G H, Qian Q, Xue H W. Semi-rolled leaf1 encodes a putative glycosylphosphatidylinositol-anchored protein and modulates rice leaf rolling by regulating the formation of bulliform cells. Plant Physiol, 2012, 159: 1488-1500. doi: 10.1104/pp.112.199968
[43]	Xiao Y H, Liu D P, Zhang G X, Tong H N, Chu C C. Brassinosteroids regulate OFP1, a DLT interacting protein, to modulate plant architecture and grain morphology in rice. Front Plant Sci, 2017, 8: 1698. doi: 10.3389/fpls.2017.01698 pmid: 29021808
[44]	Wang L, Xu J, Nian J Q, Shen N W, Lai K K, Hu J, Zeng D L, Ge C W, Fang Y X, Zhu L, Qian Q, Zhang G G. Characterization and fine mapping of the rice gene OsARVL4 regulating leaf morphology and leaf vein development. Plant Growth Regul, 2016, 78: 345-356. doi: 10.1007/s10725-015-0097-z
[45]	Huang J, Li Z Y, Zhao D Z. Deregulation of the OsmiR160 target gene OsARF18 causes growth and developmental defects with an alteration of auxin signaling in rice. Sci Rep, 2016, 6: 29938. doi: 10.1038/srep29938 pmid: 27444058
[46]	Cho S H, Yoo S C, Zhang H T, Pandeya D, Koh H J, Wang J Y, Kim G T, Paek N C. The rice narrow leaf2 and narrow leaf3 loci encode WUSCHEL-related homeobox 3A (OsWOX3A) and function in leaf, spikelet, tiller and lateral root development. New Phytol, 2013, 198: 1071-1084. doi: 10.1111/nph.2013.198.issue-4

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

标记 Marker	SNP	F₁	F₂	R
RLB	T/C	CCAACCCTAGCTTGTGATCCC	TCCAACCCTAGCTTGTGATCCT	AGGGGCCCACAAGCCTCCTG
RLC	C/G	GAATCACGAAAGCACCCTCGC	GAATCACGAAAGCACCCTCGG	GGCAAACGTGTAGATCGTCTCCC
RLF	A/C	CGTAACATACAACCATGCCTACCT	GTAACATACAACCATGCCTACCG	CGTTCAAGGGTTCACATAAGGCTTTTG
RLH	A/C	GTGGCATGTCAACTCGCGATTTATA	GGCATGTCAACTCGCGATTTATC	CAACTGAGTGGCATATATATAGTCTGTTATA
RLI	A/C	CAGAATCACTTCTTGACTACCCCAA	GAATCACTTCTTGACTACCCCAC	CAGCCTTCTAGAGCCACATTCTAGTA
RLJ	T/C	GTGCTCCTAGGAAGTAGGAGC	ATGTGCTCCTAGGAAGTAGGAGT	CCAAAAGCAAGGACGCTTCCAGG
RLK	T/C	TATTTTGAGACGAAGAGAGAATCGTG	ATTTATTTTGAGACGAAGAGAGAATCGTA	AGGCGGGTGCTTGCCCTTACAT
RLO	C/G	TTATACTAAAGCTGCATCAATTAACTTGC	TTATACTAAAGCTGCATCAATTAACTTGG	GGGCAGTACAACAAACATCAGAAATAAC

性状Trait	种子萌发15 d地上部鲜重 Aboveground fresh weight at 15 days of germination (g)	种子萌发15 d地下部鲜重 Subsurface fresh weight at 15 days of germination (g)	种子萌发15 d 根数目 Root number at 15 days of germination	种子萌发15 d 最大根长 Maximum root length at 15 days of germination (cm)	抽穗期 Heading stage (d)	成熟期 Maturity stage (d)	株高 Plant height (cm)	旗叶面积 Flag leaf area (cm²)	旗叶长 Flag leaf length (cm)	旗叶宽 Flag leaf width (cm)	穗粒数 Grain number per spike	小穗数 Spikelet number	千粒重 1000-grain weight (g)
WT	0.22±0.01^**	0.08±0.01	7±1.3	8.13±0.84	199±1	248±1	78.00±0.28^**	24.62±5.14	21.02±2.65	1.51±0.12^**	56.75±6.65^**	19.25±1.25	52.25±0.91^**
RL1	0.14±0.00	0.08±0.01	7±1.5	8.08±1.26	199±1	247±1	67.67±2.19	19.72±2.51	23.4±2.45	1.09±0.02	35.5±1.91	19.00±0.81	33.70±0.21

杂交组合 Cross combination	F₁表型及卷曲指数 Phenotype of F₁ and leaf rolled index	F₁株数 Number of plants in F₁
RL1×晋麦47 RL1/Jinmai 47	微卷(抽穗期LRIs: 0.64-0.76) Slightly curl (Heading date LRIs: 0.64-0.76)	17
晋麦47×RL1 Jinmai 47/RL1	微卷(抽穗期LRIs: 0.66-0.78) Slightly curl (Heading date LRIs: 0.66-0.78)	18
RL1×临汾5064 RL1/Linfen 5064	微卷(抽穗期LRIs: 0.61-0.76) Slightly curl (Heading date LRIs: 0.61-0.76)	17
临汾5064×RL1 Linfen 5064/RL1	微卷(抽穗期LRIs: 0.68-0.80) Slightly curl (Heading date LRIs: 0.68-0.80)	18

杂交组合 Cross combination	总株数 Number of plants	平展叶 Spread leaf	轻微卷叶 Slightly coiled leaf	高度卷叶 Highly rolled leaf	理论比例 Ratio of theory	卡方 χ²
RL1/晋麦47 RL1/Jinmai 47	176	45	94	37	1:2:1	0.03
RL1/临汾5064 RL1/Linfen 5064	173	44	95	34	1:2:1	0.01

小麦卷叶突变体RL1的生理特性及遗传研究

Physiological characteristics and genetic research of rolled leaf mutant1 (RL1) in wheat (Triticum aestivum L.)

RichHTML

PDF

摘要/Abstract

引用本文

使用本文

图/表 12

参考文献 46

相关文章 15

Metrics

本文评价

推荐阅读 0

关于本刊

在线期刊

作者中心

相关单位

联系我们

[1]	翟胜男, 曹新有, 李豪圣, 李吉虎, 李法计, 刘金栋, 夏先春, 吕莹莹, 马瑞峰, 王颖, 耿洪伟, 刘建军. 小麦Pod-A1、Pod-D1和Pod-2D位点等位变异对籽粒过氧化物酶活性的遗传效应分析[J]. 作物学报, 2026, 52(6): 1593-1603.
[2]	习千辉, 徐梓瑗, 刘梦梦, 王宏艺, 郎凯琳, 井震海, 陈锋, 赵磊. 小麦籽粒铜含量的全基因组关联分析及候选基因预测[J]. 作物学报, 2026, 52(6): 1604-1617.
[3]	毛嘉琦, 黄朋雨, 赵佳佳, 郑兴卫, 武棒棒, 郝宇琼, 屈非, 刘成, 马朋涛, 郑军. 山西小麦品种白粉病抗性评价及抗病基因分子检测[J]. 作物学报, 2026, 52(6): 1669-1681.
[4]	胡川, 赵凯男, 黄修利, 吴金芝, 任开明, 王贺正, 付国占, 黄明, 李友军. 一次灌溉下耕作方式和氮肥用量对旱地小麦产量和品质的影响[J]. 作物学报, 2026, 52(6): 1830-1846.
[5]	陈雪燕, 何华川, 李政嘉, 董新盼, 李藕琪, 刘小云, 李丹萍, 陈志伟, 刘国霞, 吕胜源, 吴印莹, 赵振东, 曹新有, 万何平. 水培盐碱复合胁迫下‘济麦60’苗期根系有机酸分泌动态变化及其转录调控机制[J]. 作物学报, 2026, 52(6): 1859-1875.
[6]	高沛阳, 李瑾璇, 董宇奎, 石玉, 张振, 张永丽. 测墒补灌下小麦分蘖发生和成穗对施氮量的响应[J]. 作物学报, 2026, 52(6): 1847-1858.
[7]	张献丰, 郭利建, 李康春, 孔斌雪, 刘玉芳, 车卓, 杨德龙. 小麦ABHD6基因家族鉴定与粒重功能标记开发[J]. 作物学报, 2026, 52(6): 1711-1727.
[8]	王壮壮, 武紫君, 张永新, 张芯源, 袁丽雪, 陈如雪, 刘世举, 段剑钊, 冯伟, 王同朝, 王永华. 豫东南黏壤潮土区水氮优化协同提高冬小麦产量和氮素利用效率[J]. 作物学报, 2026, 52(5): 1501-1521.
[9]	何万龙, 耿洪伟, 张飞飞, 米克热阿依·阿巴白克热, 罗紫洋, 李鹏程, 周钊宇, 程宇坤. 基于深度学习的小麦重要病害图像识别系统的研究[J]. 作物学报, 2026, 52(5): 1401-1417.
[10]	张振, 冯连杰, 石玉, 于振文, 张永丽. 节水补灌下不同穗型小麦产量形成差异研究[J]. 作物学报, 2026, 52(5): 1522-1535.
[11]	侯思宇, 王国璀, 韦金贵, 谢玮欣, 殷文, 樊志龙, 柴强, 胡发龙. 绿肥配施化学氮肥对西北干旱灌区小麦干物质积累及产量形成的影响[J]. 作物学报, 2026, 52(4): 1208-1219.
[12]	尚云秋, 赵竹, 陈欢, 丁永刚, 乔玉强, 李玮, 张向前, 曹承富, 杜世州. 长期定位耕作方式对雨养小麦籽粒灌浆和产量形成的影响[J]. 作物学报, 2026, 52(4): 1236-1250.
[13]	崔雪梅, 柳妍娣, 刘景辉, 米俊珍, 武俊英, 赵宝平. 不同基因型燕麦强弱势粒生理特性与产量关系研究[J]. 作物学报, 2026, 52(4): 1220-1235.
[14]	乔宇馨, 李程越, 康晓玉, 张鑫琪, 贾绍辉, 刘倩, 曹亚丽, 史鑫蕊, 郝兴宇, 李萍. 基于APSIM模型的长期免耕秸秆覆盖对旱地小麦增产效应研究[J]. 作物学报, 2026, 52(4): 1181-1192.
[15]	李灿, 张喜伟, 朱博涛, 张沛沛. 小麦GSK激酶TaSK41的功能分析及互作蛋白的筛选[J]. 作物学报, 2026, 52(3): 677-687.