甘蔗与斑茅杂交染色体组构成特征研究

doi:10.3724/SP.J.1006.2024.34100

作物学报 ›› 2024, Vol. 50 ›› Issue (3): 633-644.doi: 10.3724/SP.J.1006.2024.34100

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

甘蔗与斑茅杂交染色体组构成特征研究

薛丽¹(), 李心怡¹, 黄勇泰¹, 欧财篮¹, 吴小青¹, 余泽怀¹, 崔泽田¹, 张木清¹, 邓祖湖², 余凡¹^,^*()

¹亚热带农业生物资源保护与利用国家重点实验室 / 广西甘蔗生物学重点实验室/广西大学农学院, 广西南宁 530004
²福建农林大学农学院国家甘蔗工程技术研究中心, 福建福州 350002

收稿日期:2023-06-16 接受日期:2023-09-13 出版日期:2024-03-12 网络出版日期:2023-09-27
通讯作者: *余凡, E-mail: yufanky@163.com
作者简介:E-mail: 1825600735@qq.com
基金资助:
广西大学甘蔗专项科研项目(2022GZB006);广西甘蔗生物学重点实验室自主研究课题(GXKLSCB-20190201)

Component characterization of chromosome sets in the hybrids between sugarcane and Tripidium arundinaceum

XUE Li¹(), LI Xin-Yi¹, HUANG Yong-Tai¹, OU Cai-Lan¹, WU Xiao-Qing¹, YU Ze-Huai¹, CUI Ze-Tian¹, ZHANG Mu-Qing¹, DENG Zu-Hu², YU Fan¹^,^*()

¹State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources / Guangxi Key Laboratory of Sugarcane Biology / College of Agriculture, Guangxi University, Nanning 530004, Guangxi, China
²National Engineering Research Center for Sugarcane, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China

Received:2023-06-16 Accepted:2023-09-13 Published:2024-03-12 Published online:2023-09-27
Contact: *E-mail: yufanky@163.com
Supported by:
Sugarcane Research Foundation of Guangxi University(2022GZB006);independent fund of Guangxi Key Laboratory of sugarcane biology(GXKLSCB-20190201)

摘要/Abstract

摘要：

斑茅是甘蔗重要的野生种质资源, 渗入斑茅血缘来提高甘蔗抗性是目前甘蔗育种的重要途径之一。对甘蔗与斑茅杂交后代进行染色体分型有利于高效利用斑茅的各种优异性状。本研究利用斑茅特异引物鉴定斑茅与甘蔗杂交高世代材料的真实性, 通过荧光原位杂交对甘蔗与斑茅染色体进行分型, 以探究斑茅和甘蔗染色体在子代中的遗传与分离, 并分析其染色体组结构特征。结果表明, 杂交群体中有30份为斑茅的真实后代, 包含斑茅染色体从1~10条不等, 说明其后代群体基本服从n+n的遗传方式, 占整个真实后代群体的60%。斑茅与甘蔗发生染色体水平的重组约16.67%, 并且斑茅与不同血缘甘蔗重组的概率趋近一致。割手密种特异探针共定位结果表明, 斑茅血缘的渗入降低了近现代栽培甘蔗种中热带种血缘与重组血缘的占比, 并同时提高了割手密血缘的比例。本研究分析的甘蔗与斑茅杂交后代中不同血缘染色体组的遗传及结构特点, 为提高斑茅种质资源在甘蔗育种中的开发利用提供了细胞遗传学基础。

关键词: 甘蔗, 斑茅, 荧光原位杂交, 染色体遗传

Abstract:

T. arundinaceum (Tripidium arundinaceum) is an important wild germplasm resource of sugarcane. It is one of the important ways of sugarcane breeding to infiltrate its lineage to improve sugarcane resistance. Karyotyping of the hybrids between sugarcane and T. arundinaceum is beneficial for the efficient utilization of its various superior traits. In this study, we used species-specific primers for identifying the authenticity of the high generation hybrids between sugarcane and T. arundinaceum, and typed the sugarcane and T. arundinaceum chromosomes by fluorescence in situ hybridization to investigate the inheritance, segregation, and structural characteristics of T. arundinaceum in the offspring. These results indicated that 30 clones were true progeny with 1-10 T. arundinaceum chromosomes, indicating that their progeny population basically obeyed n+n mode of chromosome inheritance that accounted for 60% in the entire true progeny population. The probability of recombination at the chromosomal level between T. arundinaceum and sugarcane was about 16.67%, and the probability of recombination between T. arundinaceum and sugarcane of different lineages tended to be the same. Co-localization of S. spontaneum species-specific probes showed that infiltration of T. arundinaceum lineages reduced the proportion of S. officinarum and recombinant chromosomes in modern sugarcane cultivars, and simultaneously increased the proportion of S. spontaneum. In conclusion, analyzing the genetic and structural characteristics of different chromosome sets in the hybrids between sugarcane and T. arundinaceum provides a cytogenetic basis for improving the exploitation of T. arundinaceum germplasm resources in sugarcane breeding.

Key words: Saccharum, Tripidium arundinaceum, fluorescence in situ hybridization, chromosome inheritance

薛丽, 李心怡, 黄勇泰, 欧财篮, 吴小青, 余泽怀, 崔泽田, 张木清, 邓祖湖, 余凡. 甘蔗与斑茅杂交染色体组构成特征研究[J]. 作物学报, 2024, 50(3): 633-644.

XUE Li, LI Xin-Yi, HUANG Yong-Tai, OU Cai-Lan, WU Xiao-Qing, YU Ze-Huai, CUI Ze-Tian, ZHANG Mu-Qing, DENG Zu-Hu, YU Fan. Component characterization of chromosome sets in the hybrids between sugarcane and Tripidium arundinaceum[J]. Acta Agronomica Sinica, 2024, 50(3): 633-644.

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材料名称 Material name	斑茅基因组探针 Genomic probe of T. arundinaceum (µL)	割手密特异探针 Specific probe of S. spontaneum (µL)
10×DNA聚合酶I缓冲液 10×DNA polymerase I buffer	2.5	2.5
CY3/FITC标记的0.1 mmol L^-1 dNTP混合液 CY3/FITC labeled 0.1 mmol L^-1 dNTP mix	7	7
0.05 U μL^-1 DNA内切酶I 0.05 U μL^-1 DNase I	2	2
5 U μL^-1 DNA聚合酶I 5 U μL^-1 DNA polymerase I	2	2
DNA模板 DNA template	3	7.5
双蒸水 Double distilled water	8.5	4
总体系 Total system	25	25

甘蔗与斑茅杂交染色体组构成特征研究

Component characterization of chromosome sets in the hybrids between sugarcane and Tripidium arundinaceum

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序号 No.	无性系 Clone	来源 Source	序号 No.	无性系 Clone	来源 Source
1	海南92-77 Hainan 92-77	T. arundinaceum	19	崖城89-59 YCE 89-59	BC₄
2	拔地拉 Badila	S. officinarum	20	崖城89-60 YCE 89-60	BC₄
3	Np-X	S. spontaneum	21	崖城89-62 YCE 89-62	BC₄
4	柳城05-136 LC 05-136	S. cultivars	22	崖城89-63 YCE 89-63	BC₄
5	崖城05-164 YCE 05-164	BC₃	23	崖城89-66 YCE 89-66	BC₄
6	崖城89-23 YCE 89-23	BC₄	24	崖城89-67 YCE 89-67	BC₄
7	崖城89-40 YCE 89-40	BC₄	25	崖城89-70 YCE 89-70	BC₄
8	崖城89-41 YCE 89-41	BC₄	26	崖城89-75 YCE 89-75	BC₄
9	崖城89-42 YCE 89-42	BC₄	27	崖城89-76 YCE 89-76	BC₄
10	崖城89-47 YCE 89-47	BC₄	28	崖城89-77 YCE 89-77	BC₄
11	崖城89-48 YCE 89-48	BC₄	29	崖城89-79 YCE 89-79	BC₄
12	崖城89-49 YCE 89-49	BC₄	30	崖城89-81 YCE 89-81	BC₄
13	崖城89-50 YCE 89-50	BC₄	31	崖城89-85 YCE 89-85	BC₄
14	崖城89-52 YCE 89-52	BC₄	32	崖城89-86 YCE 89-86	BC₄
15	崖城89-53 YCE 89-53	BC₄	33	崖城89-88 YCE 89-88	BC₄
16	崖城89-54 YCE 89-54	BC₄	34	崖城89-89 YCE 89-89	BC₄
17	崖城89-55 YCE 89-55	BC₄	35	崖城89-90 YCE 89-90	BC₄
18	崖城89-57 YCE 89-57	BC₄	36	崖城89-91 YCE 89-91	BC₄

编号 Number	染色体数目 Number of chromosome	斑茅染色体 T. arundinaceum chromosome	割手密染色体 S. spontaneum chromosome	甘蔗属重组 Recombination of sugarcane chromosome	热带种染色体 S. officinarum chromosome
柳城05-136 LC05-136	110	0	16	30	64
崖城05-164 YCE05-164	115	15	14	12	74
崖城89-23 YCE89-23	113	7	21	19	66
崖城89-40 YCE89-40	116	9	21	16	70
崖城89-41 YCE89-41	113	7	19	20	67
崖城89-42 YCE89-42	111	7	21	12	71
崖城89-47 YCE89-47	115	8	20	16	71
崖城89-48 YCE89-48	114	8	24	18	64
崖城89-49 YCE89-49	110	9	19	15	67
崖城89-50 YCE89-50	112	10	18	17	67
崖城89-52 YCE89-52	113	9	21	17	66
崖城89-53 YCE89-53	113	6	20	18	69
崖城89-54 YCE89-54	113	8	21	20	64
崖城89-55 YCE89-55	113	9	22	18	64
崖城89-57 YCE89-57	112	7	21	16	68
崖城89-59 YCE89-59	112	7	23	20	62
崖城89-60 YCE89-60	113	8	17	14	74
崖城89-62 YCE89-62	110	7	16	16	71
崖城89-63 YCE89-63	113	8	20	15	70
崖城89-66 YCE89-66	115	0	14	16	85
崖城89-67 YCE89-67	110	7	18	16	69
崖城89-70 YCE89-70	113	7	18	14	74
崖城89-75 YCE89-75	113	6	20	20	67
崖城89-76 YCE89-76	111	7	21	18	65
崖城89-77 YCE89-77	113	9	21	15	68
崖城89-79 YCE89-79	113	5	20	14	74
崖城89-81 YCE89-81	112	1	20	19	72
崖城89-85 YCE89-85	111	7	19	14	71
崖城89-86 YCE89-86	113	8	18	15	72
崖城89-88 YCE89-88	111	8	17	19	67
崖城89-89 YCE89-89	113	9	20	16	68
崖城89-90 YCE89-90	111	8	18	16	69
崖城89-91 YCE89-91	111	6	19	13	73

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