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作物学报 ›› 2024, Vol. 50 ›› Issue (3): 779-792.doi: 10.3724/SP.J.1006.2024.31045
• 研究简报 • 上一篇
琚吉浩1(
), 马超1, 王添宁1, 吴毅1, 董钟2, 方美娥1, 陈钰姝1, 张均1,*(), 付国占1,*()
JU Ji-Hao1(), MA Chao1, WANG Tian-Ning1, WU Yi1, DONG Zhong2, FANG Mei-E1, CHEN Yu-Shu1, ZHANG Jun1,*(), FU Guo-Zhan1,*()
摘要：
过氧化物酶(Peroxidase, POD)家族成员在调节植物生长发育和响应逆境胁迫中起重要作用。为系统探究小麦(Triticum aestivum L.) TaPOD基因家族的功能及其表达模式, 本研究利用生物信息学的方法鉴定了小麦TaPOD基因家族成员, 对其理化性质、启动子顺式作用元件、进化特征做了预测分析, 并通过小麦转录组和实时荧光定量PCR (Real Time Quantitative, RT-qPCR)分析了其在不同组织、外源激素及逆境胁迫下的表达模式。结果表明, 目前基因组测序小麦中包含659个TaPOD基因家族成员, 蛋白质序列长度在206~518个氨基酸之间; 系统发育分析表明, 小麦TaPOD家族成员分为I~VII组且每组成员数量不等; 序列比对显示小麦TaPOD家族成员具有5个保守基序, 且基因结构等方面存在很大差异, 预示着功能存在多样性; 染色体定位发现其数量在小麦的21条染色体上分布不均匀, 其中2B染色体上数量最多; 通过种内共线性分析发现, 小麦TaPOD基因共有396个重复事件, 同源性较高且进化过程非常保守, 主要通过片段复制和串联复制进行扩增, 且Ka/Ks比率显示仅有4对家族成员受到了正向的自然选择压力; 顺式作用元件分析表明, 上游2 kb区域中存在23种与生长发育和抗逆性相关的结合元件; 基因表达模式分析显示, 86.5% TaPOD基因在小麦根系中表达量较高; 通过RT-qPCR检测发现TaPOD基因表达量与激素诱导和非生物胁迫密切相关。上述结果为深入研究TaPOD基因在调控小麦生长发育与逆境胁迫中的功能提供初步的理论基础。
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