作物学报 ›› 2025, Vol. 51 ›› Issue (4): 1110-1117.doi: 10.3724/SP.J.1006.2025.42042
朱建平1,2,3(), 李文奇1,2,3, 许扬1,2,3, 王芳权1,2,3, 李霞1,2,3, 蒋彦婕1,2,3, 范方军1,2,3, 陶亚军1,2,3, 陈智慧1,2,3, 吴莹莹1,2,3, 杨杰1,2,3,*(
)
ZHU Jian-Ping1,2,3(), LI Wen-Qi1,2,3, XU Yang1,2,3, WANG Fang-Quan1,2,3, LI Xia1,2,3, JIANG Yan-Jie1,2,3, FAN Fang-Jun1,2,3, TAO Ya-Jun1,2,3, CHEN Zhi-Hui1,2,3, WU Ying-Ying1,2,3, YANG Jie1,2,3,*(
)
摘要:
从日本晴60Co诱变突变体库中筛选到一份粉质胚乳突变体we2 (white endosperm2), 对其表型和理化性质进行分析, 并利用we2与9311杂交获得的F2群体对目标基因进行精细定位。we2胚乳表现为白色粉质, 淀粉颗粒排列疏松且不规则; 千粒重、总淀粉和直链淀粉含量显著下降, 脂肪含量显著上升。遗传分析表明we2粉质胚乳性状受单个隐性基因控制。利用we2/9311 F2群体进行基因定位, WE2被定位在第6染色体长臂P38和P39之间约244 kb区间内, 该区间包含27个开放阅读框(Open reading frames, ORFs)。qRT-PCR结果显示, we2胚乳中淀粉合成相关基因表达显著下调。本研究为WE2的克隆和功能分析奠定了基础。
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