作物学报 ›› 2025, Vol. 51 ›› Issue (7): 1736-1746.doi: 10.3724/SP.J.1006.2025.44141
尹雨萌1,**,王雁楠1,**,康志河1,乔守晨1,卞倩倩1,李亚蔚1,曹郭郑1,赵国瑞1,徐丹丹2,杨育峰1,*
YIN Yu-Meng1,**,WANG Yan-Nan1,**,KANG Zhi-He1,QIAO Shou-Chen1,BIAN Qian-Qian1,LI Ya-Wei1,CAO Guo-Zheng1,ZHAO Guo-Rui1,XU Dan-Dan2,YANG Yu-Feng1,*
摘要:
本研究通过分析前期的薯皮色变异转录组数据,克隆得到IbGSTU7基因,该基因开放阅读框(ORF)全长660 bp,编码219个氨基酸。蛋白序列分析表明,IbGSTU7为酸性、亲水性、稳定性蛋白,且与甘薯近缘野生种Ipomoea triloba的亲缘关系最近,定位于细胞质中。通过实时荧光定量PCR(qRT-PCR)对IbGSTU7基因的表达特征进行分析发现,IbGSTU7在甘薯主要组织中均有表达,且在薯皮中的表达量最高。此外,干旱、盐胁迫以及外源脱落酸(ABA)均可诱导IbGSTU7基因上调表达。通过农杆菌转化法对IbGSTU7在甘薯中进行过表达发现,过表达植株的薯皮花青苷含量以及花青苷合成途径相关基因IbPAL与IbUFGT的表达量均显著提高。同时,PEG模拟干旱胁迫下过表达植株表现出更强的抗旱性,鲜重和根长显著高于野生型对照,过氧化氢(H2O2)含量显著降低,活性氧(ROS)清除相关基因IbMDHAR和IbPOD显著上调表达。本研究为进一步探索IbGSTU7基因在甘薯花青苷积累和干旱胁迫应答中的功能提供了新的理论支撑。
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