豆科作物AOX基因鉴定及其在普通菜豆响应非生物胁迫中的表达模式研究

doi:10.3724/SP.J.1006.2025.44170

作物学报 ›› 2025, Vol. 51 ›› Issue (7): 1769-1783.doi: 10.3724/SP.J.1006.2025.44170

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

豆科作物AOX基因鉴定及其在普通菜豆响应非生物胁迫中的表达模式研究

闫知兰¹，赵芹¹，常甜达¹，王一鸣¹，王碧辉¹，王鹏¹，黄春国¹，张会²，王利祥¹，郝晓鹏^3,* ，赵波^1,*

1山西农业大学农学院 / 山西省后稷实验室, 山西太原030031; 2山西农业大学生命科学学院, 山西太谷030801; 3山西农业大学农业基因资源研究中心 / 杂粮种质资源发掘与遗传改良山西省重点实验室, 山西太原030031

收稿日期:2024-10-06 修回日期:2025-04-27 接受日期:2025-04-27 出版日期:2025-07-12 网络出版日期:2025-05-13
基金资助:
本研究由国家重点研发计划项目(2023YFD1202700, 2023YFD1202703), 国家自然科学基金项目(32301778), 山西省博士毕业生、博士后研究人员来晋工作奖励经费科研项目(SXBYKY2023004), 山西省基础研究计划(自由探索类)项目(202403021211051, 202203021222147), 山西省后稷实验室自主立项课题(202304010930003-03)和山西省科技创新人才团队专项(202204051002013)资助。

Genome-wide identification and characterization of Alternative oxidase (AOX) genes in leguminous crops and their expression patterns in response to abiotic stresses in common bean

YAN Zhi-Lan¹,ZHAO Qin¹,CHANG Tian-Da¹,WANG Yi-Ming¹,WANG Bi-Hui¹,WANG Peng¹,HUANG Chun-Guo¹,ZHANG Hui²,WANG Li-Xiang¹,HAO Xiao-Peng^3,*,ZHAO Bo^1,*

1 College of Agriculture, Shanxi Agricultural University / Shanxi Houji Laboratory, Taiyuan 030031, Shanxi, China; 2 College of Life Sciences, Shanxi Agricultural University, Taigu 030801, Shanxi, China; 3 Agricultural Gene Resources Research Center, Shanxi Agricultural University / Key Laboratory of Germplasm Resources Exploration and Genetic Improvement of Coarse Cereals, Taiyuan 030031, Shanxi, China

Received:2024-10-06 Revised:2025-04-27 Accepted:2025-04-27 Published:2025-07-12 Published online:2025-05-13
Supported by:
This study was supported by National Key R&D Program of China (2023YFD1202700, 2023YFD1202703), the National Natural Science Foundation of China (32301778), the Shanxi Province’s Reward Fund for Doctoral Graduates and Postdoctoral Researchers to Work in Shanxi Province (SXBYKY2023004), the Shanxi Provincial Basic Research Program-Free Exploration Category (202403021211051, 202203021222147), the Shanxi Houji Laboratory Self-initiated Research Projects (202304010930003-03), and the Special Fund for Science and Technology Innovation Teams of Shanxi Province (202204051002013).

摘要/Abstract

摘要：

交替氧化酶(alternative oxidase, AOX)是一种重要的线粒体末端氧化酶，在植物抵御非生物胁迫中扮演着关键角色。豆科作物作为植物蛋白的重要来源，在农业可持续发展中发挥着重要作用。然而，近年来频发的极端气候对豆科作物的产量和品质造成了严重影响，提高其耐逆性已成为亟待解决的问题。本研究对大豆、普通菜豆、宽叶菜豆和绿豆4种豆科作物的AOX基因家族进行了系统性的鉴定和分析，包括蛋白结构、理化性质、启动子顺式作用元件，以及普通菜豆AOX基因在多种非生物胁迫下的表达模式。家族成员鉴定发现，大豆、普通菜豆、宽叶菜豆和绿豆分别包含4个、3个、3个和3个AOX基因，亲缘关系分析将其划分为3个不同的亚族。顺式作用元件分析结果显示，豆科作物AOX基因启动子区域存在很多响应激素和逆境胁迫的顺式作用元件。对普通菜豆AOX基因在不同非生物胁迫下的表达模式进行分析发现，其AOX同源基因对不同非生物胁迫刺激响应存在差异，表现出多样的时序变化模式。其中，PvAOX1A;2受多种非生物胁迫的诱导显著上调表达。而PvAOX2;2_2受盐胁迫和高温胁迫诱导显著上调表达，PvAOX2;2_1受冷胁迫诱导显著上调表达。综上，普通菜豆AOX基因可能在其应答非生物胁迫中发挥着重要作用。本研究相关结果不仅为深入探究豆科作物AOX基因的生物学功能提供了重要线索，也为普通菜豆耐逆性的分子改良提供了潜在的靶基因。

关键词: 普通菜豆, AOX, 豆科作物, 非生物胁迫, 表达模式

Abstract:

Alternative oxidase (AOX), a key terminal oxidase in the mitochondrial electron transport chain, plays a pivotal role in plant response to various abiotic stresses. Leguminous crops are not only important sources of plant-based protein but also contribute significantly to sustainable agricultural development. However, their yield and quality have been increasingly compromised by frequent extreme climatic events, highlighting the urgent need to enhance their stress tolerance. In this study, we systematically identified and analyzed the AOX gene family in four leguminous crops: soybean, common bean, tepary bean, and mung bean. Our analysis included assessments of protein structure, physicochemical properties, promoter cis-acting elements, and the expression patterns of AOX genes in common bean under different abiotic stress treatments. We identified four, three, three, and three AOX genes in soybean, common bean, tepary bean, and mung bean, respectively. Phylogenetic analysis grouped these genes into three distinct subfamilies. Cis-element analysis of promoter regions revealed an abundance of hormone-responsive and stress-responsive element. Expression profiling in common bean demonstrated that AOX genes exhibit differential responses to various abiotic stresses, with distinct temporal expression patterns. Notably, PvAOX1A;2 was strongly upregulated under multiple stress conditions. PvAOX2;2_2 showed high induction under salt and heat stress, while PvAOX2;2_1 was significantly upregulated in response to cold stress. Collectively, these results suggest that AOX genes play important roles in the abiotic stress responses in common bean. This study provides valuable insights into the functional characteristics of AOX genes in leguminous crops and identifies potential candidate genes for the molecular improvement of stress tolerance in common bean.

Key words: common bean, AOX, leguminous crops, abiotic stress, expression pattern

闫知兰, 赵芹, 常甜达, 王一鸣, 王碧辉, 王鹏, 黄春国, 张会, 王利祥, 郝晓鹏, 赵波. 豆科作物AOX基因鉴定及其在普通菜豆响应非生物胁迫中的表达模式研究[J]. 作物学报, 2025, 51(7): 1769-1783.

YAN Zhi-Lan, ZHAO Qin, CHANG Tian-Da, WANG Yi-Ming, WANG Bi-Hui, WANG Peng, HUANG Chun-Guo, ZHANG Hui, WANG Li-Xiang, HAO Xiao-Peng, ZHAO Bo. Genome-wide identification and characterization of Alternative oxidase (AOX) genes in leguminous crops and their expression patterns in response to abiotic stresses in common bean[J]. Acta Agronomica Sinica, 2025, 51(7): 1769-1783.

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豆科作物AOX基因鉴定及其在普通菜豆响应非生物胁迫中的表达模式研究

Genome-wide identification and characterization of Alternative oxidase (AOX) genes in leguminous crops and their expression patterns in response to abiotic stresses in common bean

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