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作物学报 ›› 2023, Vol. 49 ›› Issue (4): 938-954.doi: 10.3724/SP.J.1006.2023.24066
孙全喜1(
), 苑翠玲1, 牟艺菲1, 闫彩霞1, 赵小波1, 王娟1, 王奇1, 孙慧2, 李春娟1, 单世华1,*()
SUN Quan-Xi1(), YUAN Cui-Ling1, MOU Yi-Fei1, YAN Cai-Xia1, ZHAO Xiao-Bo1, WANG Juan1, WANG Qi1, SUN Hui2, LI Chun-Juan1, SHAN Shi-Hua1,*()
摘要：
SWEET (sugars will eventually be exported transporter)蛋白是一类结构保守、不依赖能量的糖转运蛋白, 在植物生长发育、响应生物/非生物逆境胁迫等生理过程发挥重要作用。目前, 尚未见花生SWEET基因相关报道。本研究首次全基因组挖掘了花生SWEET基因, 对其分子特征及表达模式进行了细致分析。结果表明, 栽培种花生和2个祖先野生种基因组分别存在55、25、28个SWEET基因, 随机不均匀分布在各染色体上。来源于野生种和栽培种的同源基因在染色体位置相近, 但也存在个别缺失, 这验证了花生野生种和栽培种的进化关系, 也暗示了基因组复制加倍过程中存在同源基因的丢失或扩张。基因内含子-外显子数目和位置以及启动子中顺式作用元件种类和数量均存在差异, 暗示了花生SWEET基因生物学功能的多样性。系统进化分析将花生SWEET基因分为4个亚家族Clade I~Clade IV, 同一亚家族同一分支的基因具有相似的外显子-内含子结构。分析Clevenger等组织表达谱发现部分基因表现为组织优势表达, 这为深入了解SWEET基因行使功能部位提供了参考。此外, 基于课题组前期发表的干旱和高盐胁迫转录组分析和RT-qPCR验证, 我们挖掘出AhSWEET3a和AhSWEET4e等响应花生干旱或高盐胁迫的基因, 功能有待进一步鉴定。研究结果为下一步深入分析花生SWEET基因功能提供了理论参考。
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