作物学报 ›› 2023, Vol. 49 ›› Issue (4): 955-965.doi: 10.3724/SP.J.1006.2023.24089
徐子寅1(), 于晓玲2,3, 邹良平2,3, 赵平娟2,3, 李文彬2,3, 耿梦婷1,*(
), 阮孟斌2,3,*(
)
XU Zi-Yin1(), YU Xiao-Ling2,3, ZOU Liang-Ping2,3, ZHAO Ping-Juan2,3, LI Wen-Bin2,3, GENG Meng-Ting1,*(
), RUAN Meng-Bin2,3,*(
)
摘要:
Myeloblastosis (MYB)类转录因子在植物对非生物胁迫的响应过程中起重要调控作用。本研究在分析栽培木薯MYB家族成员表达模式的基础上, 筛选并克隆到了一个R2R3-MYB转录因子基因MeMYB60。基因表达特性分析表明, 该基因在叶片特异表达, 受干旱、低温负调控, 同时对ABA处理也有响应。启动子活性分析发现, MeMYB60可以在保卫细胞表达, 预示着该转录因子基因的表达可能与木薯气孔开闭调节有关。MeMYB60编码蛋白主要定位于细胞核中, 具有转录激活活性, 其转录激活结构域在蛋白C端第194~343氨基酸残基范围内。以MeMYB60蛋白N端第1~194氨基酸残基片段为诱饵, 从干旱胁迫后木薯叶片的cDNA文库中筛选到18种可能与MeMYB60互作的蛋白, 酵母双杂确定了MeCatlase1和MeCatalase2分别与MeMYB60存在互作关系。本研究为深入研究转录因子MeMYB60在木薯响应非生物胁迫过程中的功能并解析其调控网络奠定了基础。
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