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马铃薯III类POD基因家族的全基因组鉴定及其表达谱分析

杨飚，杜帅康，张继旺，石瑛*，张丽莉*   

  1. 东北农业大学农学院马铃薯研究所, 黑龙江哈尔滨150030
  • 收稿日期:2025-08-01 修回日期:2025-10-30 接受日期:2025-10-30 网络出版日期:2025-11-07
  • 基金资助:
    本研究由国家自然科学基金项目(31601355)，财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-09)资助。

Genome-wide identification of class III POD gene family in potato and its expression profile analysis

Yang Biao,Du Shuai-Kang,Zhang Ji-Wang,Shi Ying*,Zhang Li-Li*   

  1. Potato Research Institute, College of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China
  • Received:2025-08-01 Revised:2025-10-30 Accepted:2025-10-30 Published online:2025-11-07
  • Supported by:
    This study was supported by the National Natural Science Foundation of China (31601355) and the the China Agriculture Research System of MOF and MARA (CARS-09).

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摘要： III类过氧化物酶(EC 1.11.1.7)是植物特异性氧化还原酶，广泛分布于生物体内，催化作为电子受体的过氧化氢(H2O2)与多种电子供体之间的氧化还原反应，是植物在胁迫条件下酶防御系统的关键酶之一。马铃薯(Solanum tuberosum L.)是茄科茄属的一年生草本植物，目前关于马铃薯POD基因家族(StPODs)的功能研究鲜有报道。本研究通过生物信息学的方法对StPODs基因家族成员进行了分析，探究其在多种非生物胁迫下的表达模式。马铃薯全基因组中共鉴定出148个StPODs基因家族成员，并根据基因在染色体上位置顺序依次命名为StPOD1~StPOD148。148个StPODs蛋白的长度为76~914个氨基酸不等，分子质量在83.64~101.32 kD之间；通过保守基序和结构域分析148个StPODs基因结构发现，所有StPODs基因均具有5个高度保守的Motif (Motif 1、Motif 2、Motif 3、Motif 4和Motif 5)和3个保守结构域(plant_peroxidase_like superfamily、secretory_peroxidase和PLN03030 superfanmily)。微阵列数据用于进一步的表达谱分析，多个StPODs基因受盐胁迫、干旱胁迫和高温胁迫后其表达量显著增加，其中响应盐胁迫的差异表达基因数量最多(64个)；经脱落酸(ABA)、生长素(IAA)、赤霉素(GA3)和苄氨基嘌呤(BAP)处理后分别诱导了多个StPODs差异表达基因，其中85个StPODs基因受ABA诱导后差异表达。此外，本试验通过分析耐旱型马铃薯材料“A90”和干旱敏感型材料“A163”的表达谱，筛选出14个StPODs耐旱候选基因，它们在耐旱型材料和干旱敏感型材料之间的表达量表现出相反的趋势，将表达量趋势相差最大的6个候选基因在酒酿酵母中异源表达，证实了StPOD23和StPOD53基因参与酵母细胞的渗透调节反应。研究结果可为StPOD基因后续功能研究提供理论基础。

关键词: 过氧化物酶, 马铃薯, 非生物胁迫, 干旱胁迫, 表达谱分析

Abstract:

Class III peroxidases (EC 1.11.1.7) are plant-specific oxidoreductases widely distributed across plant species. They catalyze redox reactions between hydrogen peroxide (H2O2), serving as an electron acceptor, and various electron donors, playing a critical role in plant responses to diverse environmental stresses. Potato (Solanum tuberosum L.), a herbaceous annual of the Solanum genus in the Solanaceae family, has seen limited functional research on its peroxidase (POD) gene family (StPODs). In this study, we conducted a comprehensive bioinformatics analysis of the StPOD gene family to explore their expression patterns under various abiotic stresses. A total of 148 StPOD genes were identified in the potato genome and named StPOD1–StPOD148 based on their chromosomal positions. These StPOD proteins ranged from 76 to 914 amino acids in length, with an average length of 310 amino acids, and molecular weights ranging from 8.36 to 101.32 kDa. Functional analyses based on conserved motifs and structural domains revealed that all StPODs contained five highly conserved motifs (Motifs 1–5) and three conserved domains: plant_peroxidase_like superfamily, secretory_peroxidase, and PLN03030 superfamily. Microarray data were used to analyze their expression profiles under stress conditions. Many StPODs showed significantly increased expression in response to salt, drought, and high temperature stresses, with the highest number of differentially expressed genes (64) observed under salt stress. Additionally, several StPODs were induced by plant hormone treatments, including abscisic acid (ABA), indole-3-acetic acid (IAA), gibberellic acid (GA3), and benzylaminopurine (BAP), with 85 StPOD genes showing differential expression following ABA induction. Furthermore, by comparing expression profiles between the drought-tolerant potato line “A90” and the drought-sensitive line “A163”, 14 StPODs were identified as candidate drought-tolerance genes, showing opposite expression trends between the two lines. Six genes with the most pronounced differential expression were heterologously expressed in Saccharomyces cerevisiae, and functional analysis confirmed that StPOD23 and StPOD53 are involved in osmoregulatory responses in yeast cells. These findings provide a theoretical foundation for future functional studies of StPOD genes.

Key words: peroxidase, potato, abiotic stresses, drought stress, expression profile analysis

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