不同马铃薯品种块茎创伤愈合能力的比较

doi:10.3724/SP.J.1006.2025.54027

摘要/Abstract

摘要：

马铃薯块茎的创伤愈合能力因品种而异，本研究以5个四倍体马铃薯栽培品种‘滇薯47号’(D47)、‘滇薯14023’(14023)、‘滇薯1208’(1208)、‘合作88’(C88)和‘青薯9号’(Q9)为材料，观察软木脂和木质素在损伤块茎中的积累，并测定苯丙烷途径关键酶活性和代谢产物含量，以及创伤对木栓质合成相关基因相对表达水平的影响，利用隶属函数法对5个马铃薯品种的创伤愈合能力进行综合评价。结果表明，5个马铃薯品种损伤块茎中的PAL、4CL、C4H、POD酶活性和StPAL、StPOD、St4CL、StC4H相对基因表达量在愈伤前期(0~2 d)快速积累，到愈伤中后期(4~8 d)持续增加且达到最大值；随着创伤时间的增加，木质素、类黄酮、总酚含量增加，SPP和SPA快速沉积，形成创伤周皮。在整个创伤愈合过程中，D47的失重率最低，1208和C88的失重率最高；D47的SPA、SPP和木质素层沉积速度较快，在创伤后6 d达到最大值，14023和1208的沉积速度较慢；此外D47和C88具有较高的PAL、POD和C4H酶活性；StPAL、StPOD、StC4H相对基因表达量在D47和Q9中显著高于其余品种(P < 0.05)；而14023和1208的PAL和POD酶活性较低；D47的木质素、总酚和类黄酮含量在整个创伤愈合过程中较高，在1208和14023中的积累较低。通过5个马铃薯品种在整个创伤愈合过程中(创伤后0~8 d)的18个创伤愈合指标进行主成分分析，筛选出失重率、SPA细胞层厚度、SPP细胞层厚度、木质素细胞层厚度及StPAL、StC4H和StPOD相对表达水平作为评价马铃薯创伤愈合能力的代表性指标，根据隶属函数分析法对不同品种创伤愈合能力综合排序为D47 (3.9808) > Q9 (3.5767) > C88 (3.4663) > 1208 (3.3546) > 14023 (2.0241)，且苯丙烷代谢途径在马铃薯块茎愈合中起重要作用。

关键词: 马铃薯品种, 创伤愈合, 苯丙烷代谢, 主成分分析, 隶属函数

Abstract:

The wound healing ability of potato tubers varies among cultivars. In this study, five tetraploid potato cultivars “Dianshu 47” (D47), “Dianshu 14023” (14023), “Dianshu 1208” (1208), “Cooperation-88” (C88), and “Qingshu 9” (Q9) were used, as experimental materials. The accumulation of suberin and lignin at wound sites was observed, along with the activity of key enzymes and metabolite contents involved in the phenylpropanoid pathway, as well as the relative expression levels of genes related to suberin biosynthesis. The wound healing capacity of the five cultivars was comprehensively evaluated using the membership function method. The results showed that the activities of PAL, 4CL, C4H, and POD, as well as the relative expression levels of StPAL, StPOD, St4CL, and StC4H, increased rapidly during the early stage of wound healing (0–2 d) and peaked during the middle to late stages (4–8 d). As healing progressed, the contents of lignin, flavonoids, and total phenolics increased, while SPP and SPA were rapidly deposited to form the wound periderm. Among the whole cultivars, D47 exhibited, the lowest weight loss rate, 1208 and C88 showed the highest. The deposition rates of SPA, SPP, and lignin layers in D47 were relatively rapid, reaching maximum levels at 6 days post-injury, while deposition in 14023 and 1208 was comparatively slower. Additionally, D47 and C88 showed higher activities of PAL, POD, and C4H, and therelative expression levels of StPAL, StPOD, and StC4H were significantly higher in D47 and Q9 than in the other cultivars (P < 0.05), In contrast, 14023 and 1208 exhibited lower PAL and POD activities. During the wound-healing process, lignin, total phenolics, and flavonoid contents were consistently higher in D47 and lower in 1208 and 14023. Principal component analysis of 18 wound-healing indicators across the 0–8 day period identified weight loss rate, SPA cell layer thickness, SPP cell layer thickness, lignin layer thickness, and the relative expression levels of StPAL, StC4H, and StPOD as representative indicators for evaluating wound healing ability. Based on membership function analysis, the overall ranking of wound healing ability was: D47 (3.9808) > Q9 (3.5767) > C88 (3.4663) > 1208 (3.3546) > 14023 (2.0241). These findings suggest that the phenylpropanoid metabolic pathway plays a key role in the wound suberization process of potato tubers.

Key words: potato varieties, wound healing, phenylpropane metabolism, principal component analysis, membership function

尹丽娜, 张锐, 陈国欢, 白磊, 李俊, 郭华春, 杨芳. 不同马铃薯品种块茎创伤愈合能力的比较[J]. 作物学报, 0, (): 0-.

YIN Li-Na, ZHANG Rui, CHEN Guo-Huan, BAI Lei, LI Jun, GUO Hua-Chun, YANG Fang. Comparison of wound healing capacity of tubers of different potato varieties[J]. Acta Agronomica Sinica, 0, (): 0-.

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