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作物学报 ›› 2015, Vol. 41 ›› Issue (01): 145-153.doi: 10.3724/SP.J.1006.2015.00145

• 耕作栽培·生理生化 • 上一篇    下一篇

绿豆种质资源苗期抗旱性鉴定

王兰芬,武晶,景蕊莲,程须珍,王述民   

  1. 中国农业科学院作物科学研究所 / 农作物基因资源与基因改良国家重大科学工程, 北京 100081
  • 收稿日期:2014-05-22 修回日期:2014-09-16 出版日期:2015-01-12 网络出版日期:2014-09-26
  • 通讯作者: 王述民, E-mail: wangshumin@caas.cn
  • 基金资助:

    本研究由国家现代农业产业技术体系建设专项(CARS-09), 中国农业科学院科技创新工程项目和国家科技支撑计划项目(2013BAD05-2-4)资助。

Drought Resistance Identification of Mungbean Germplasm Resources at Seedlings Stage

WANG Lan-Fen,WU Jing,JING Rui-Lian,CHENG Xu-Zhen,WANG Shu-Min?   

  1. Institute of Crop Science, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China?
  • Received:2014-05-22 Revised:2014-09-16 Published:2015-01-12 Published online:2014-09-26
  • Contact: 王述民, E-mail: wangshumin@caas.cn

摘要:

鉴定筛选苗期抗旱绿豆种质, 对改良绿豆品种抗旱性、促进绿豆产业发展具有重要意义, 同时, 也为“绿豆抗旱性鉴定评价技术规范”的制定提供方法和信息支撑。本研究以70份绿豆种质为材料, 采用苗期反复干旱法,测定幼苗存活率、萎蔫指数、株高、叶片鲜重、叶片干重、叶片含水量、生物量和胁迫指数等指标, 分析各指标间的相关性, 结果显示第1次旱胁迫后的幼苗存活率与第2次旱胁迫后的幼苗存活率呈极显著正相关; 萎蔫指数、株高与幼苗存活率呈极显著负相关,故遴选出第1次旱胁迫幼苗存活率、萎蔫指数和株高为绿豆苗期抗旱性评价的适宜指标。采用隶属函数法, 综合分析划级, 获得高抗旱种质16份、抗旱种质20份, 中抗种质23份、敏感种质8份和极敏感种质3份。以第1次旱胁迫幼苗存活率、萎蔫指数和胁迫株高分别评价绿豆的抗旱性并与综合评价结果相比较,一致率分别为70.0%、58.6%和51.4%。认为第1次旱胁迫幼苗存活率可以作为大规模绿豆种质苗期抗旱筛选的鉴定指标。

关键词: 绿豆, 苗期, 反复干旱, 抗旱性, 相关性分析, 隶属函数

Abstract:

It is significant to identify and screen drought resistant mungbean germplasm resources at seedling stage for mungbean cultivar improvement and production in China, at the same time, which could provide some reference methods and basic information for developing “the technical specification of identification and evaluation for drought resistance in mungbean”. The indices including survival rate of seedlings, wilt index, plant height, weight of fresh and dry leaf, relative water content, biomass weight and stress index were measured with the method of repeat drought stress in 70 accessions of mungbean resources. The survival rate of seedlings had significantly positive correlation between the first and the second drought stress, both wilt index and plant height had significantly negative correlation with survival rate. The survival rate of seedling under the first drought stress, wilt index and plant height were selected as suitable indices for drought resistance identification and evaluation based on correlation analysis. The various resistant accessions of mungbean germplasm resources screened by subordinative function analysis included 16 highly resistant, 20 resistant, 23 moderately resistant, eight susceptible and three highly susceptible. The drought resistance was evaluated based on the survival rate of seedling under the first drought stress, wilt index and plant height with the concordance rate of 70.0%, 58.6%, and 51.4% respectively as compared to the comprehensive evaluation. The survival rate of seedling under the first drought stress was recommended as a drought resistance index in screening a large number of germplasm resources ofmungbean.

Key words: Mungbean, Seedling stage, Repeat drought, Drought resistance, Correlation analysis, Subordinative function analysis

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