MeERF6-MePAP2模块响应低温胁迫的分子机制研究

doi:10.3724/SP.J.1006.2025.54025

作物学报 ›› 2025, Vol. 51 ›› Issue (11): 2971-2982.doi: 10.3724/SP.J.1006.2025.54025

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

MeERF6-MePAP2模块响应低温胁迫的分子机制研究

艾力^1,2，李梦桃^1,2，卢发宝^1,2，刘晓晨^1,2，麦伟涛^1,2，周新成^2,3,*，陈新^2,3,*

1海南大学热带农林学院, 海南海口570228; 2热带作物生物育种全国重点实验室 / 中国热带农业科学院热带生物技术研究所, 海南海口571101; 3中国热带农业科学院三亚研究院, 海南三亚572025

收稿日期:2025-02-21 修回日期:2025-07-09 接受日期:2025-07-09 出版日期:2025-11-12 网络出版日期:2025-07-14
通讯作者: 周新成, E-mail: zhouxincheng@itbb.org.cn; 陈新, E-mail: chenxin@itbb.org.cn
基金资助:
本研究由热带作物生物育种全国重点实验室创新团队科研项目(NKLTCBCXTD07)和财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-11-HNCX)资助。

Molecular mechanism of the MeERF6-MePAP2 module in response to low-temperature stress

AI Li^1,2,LI Meng-Tao^1,2,LU Fa-Bao^1,2,LIU Xiao-Chen^1,2,MAI Wei-Tao^1,2,ZHOU Xin-Cheng^2,3,*,CHEN Xin^2,3,*

1 College of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, Hainan, China; 2 National Key Laboratory of Tropical Crop Biological Breeding / Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, China; 3 Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya 572025, Hainan, China

Received:2025-02-21 Revised:2025-07-09 Accepted:2025-07-09 Published:2025-11-12 Published online:2025-07-14
Supported by:
This study was supported by the National Key Laboratory of Tropical Crop Biotechnology Innovation Team Research Project (NKLTCBCXTD07) and the China Agriculture Research System of MOF and MARA (CARS-11-HNCX).

摘要/Abstract

摘要：

以木薯品种SC124的自交一代分离群体为研究对象，通过集团分离分析法(BSA)和基因组重测序，定位并克隆了MYB类转录因子基因MePAP2。研究发现，MePAP2启动子区域存在一段84 bp的插入缺失，该插入缺失与紫色叶柄和绿色叶柄性状的分离显著相关，并与植株在低温环境下的生长势密切相关。进一步以MePAP2启动子为诱饵利用酵母单杂交试验，筛选到AP2/ERF家族转录因子基因MeERF6，并通过双荧光素酶试验和活体成像技术确定MeERF6能够直接结合MePAP2启动子并正向调控其转录活性。MeERF6在低温胁迫下的响应时间早于MePAP2，且其在叶柄组织中的转录活性显著高于其他部位。本研究揭示了MeERF6-MePAP2模块在木薯低温胁迫响应中的分子调控机制，为培育耐低温木薯新品种提供了重要的理论依据和基因资源。

关键词: 木薯, 低温胁迫, MePAP2, MeERF6, 酵母单杂

Abstract:

Using a segregating population derived from the self-pollination of the cassava variety SC124, we employed Bulked Segregant Analysis (BSA) combined with genome re-sequencing to map and clone the MYB-family transcription factor MePAP2. Genetic analysis revealed an 84 bp insertion-deletion (InDel) in the promoter region of MePAP2, which was significantly associated with the segregation of the purple/green petiole trait and correlated with plant growth vigor under low-temperature conditions. A yeast one-hybrid assay using the MePAP2 promoter as bait identified the AP2/ERF-family transcription factor MeERF6. Dual-luciferase reporter assays and in vivo imaging demonstrated that MeERF6 directly binds to the MePAP2 promoter and positively regulates its transcription. Notably, MeERF6 responded to low-temperature stress earlier than MePAP2, and its transcriptional activity was significantly higher in petiole tissue compared to other organs. Collectively, these findings elucidate a hierarchical regulatory pathway involving the MeERF6–MePAP2 module in cassava’s response to low-temperature stress, providing valuable genetic targets for the molecular breeding of cold-tolerant cassava varieties.

Key words: cassava, low-temperature stress, MePAP2, MeERF6, yeast one-hybrid

艾力, 李梦桃, 卢发宝, 刘晓晨, 麦伟涛, 周新成, 陈新. MeERF6-MePAP2模块响应低温胁迫的分子机制研究[J]. 作物学报, 2025, 51(11): 2971-2982.

AI Li, LI Meng-Tao, LU Fa-Bao, LIU Xiao-Chen, MAI Wei-Tao, ZHOU Xin-Cheng, CHEN Xin. Molecular mechanism of the MeERF6-MePAP2 module in response to low-temperature stress[J]. Acta Agronomica Sinica, 2025, 51(11): 2971-2982.

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MeERF6-MePAP2模块响应低温胁迫的分子机制研究

Molecular mechanism of the MeERF6-MePAP2 module in response to low-temperature stress

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