欢迎访问作物学报,今天是

作物学报 ›› 2008, Vol. 34 ›› Issue (06): 984-990.doi: 10.3724/SP.J.1006.2008.00984

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

W6基因的过表达提高转基因烟草的耐盐性

鲁燕1,2;徐兆师2,*;张瑞越2,3;刘丽2;李连城2;陈明2;叶兴国2;陈耀锋1;马有志2,*   

  1. 1 西北农林科技大学农学院, 陕西杨凌712100; 2 中国农业科学院作物科学研究所 / 国家农作物基因资源与遗传改良重大科学工程 / 农业部作物遗传育种重点开放实验室, 北京100081; 3 淮阴师范学院生物系, 江苏淮安223300
  • 收稿日期:2007-05-29 修回日期:1900-01-01 出版日期:2008-06-12 网络出版日期:2008-06-12
  • 通讯作者: 徐兆师

Overexpression of W6 Gene Increases Salt Tolerance in Transgenic To-bacco Plants

LU Yan12,XU Zhao-Shi2*,ZHANG Rui-Yue23,LIU Li2,LI Lian-Cheng2,CHEN Ming2,YE Xing-Guo2,CHEN Yao-Feng1,MA You-Zhi2*   

  1. 1 College of Agriculture, Northwest A&F University, Yangling 712100, Shaanxi; 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sci-ences / National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Crop Genetics and Breeding, Ministry of Ag-riculture, Beijing 100081; 3 Department of Biology, Huaiyin Normal College, Huai’an 223300, Jiangsu, China
  • Received:2007-05-29 Revised:1900-01-01 Published:2008-06-12 Published online:2008-06-12
  • Contact: XU Zhao-Shi

摘要: W6基因是由普通小麦地方品种小白麦cDNA文库中克隆获得的ERF类转录因子, 属于ERF IV家族。将W6基因构建在由组成型CaMV35S强启动子控制的双子叶转化载体pBI121上, 通过农杆菌介导法将其转入烟草新华1号。利用RT-PCR技术分析W6基因的过量表达对下游抗性基因表达水平的影响, 并测定高盐胁迫下转基因烟草的SOD酶活性和叶绿素含量。结果显示, 所检测的转基因烟草阳性株中W6基因均有不同程度的表达, 其耐盐性明显提高。W6基因的表达诱导并增强了含有GCC box的PR(pathogenesis-related)基因和含有DRE/CRT元件的COR/RD基因的表达。在高盐胁迫下, 转基因烟草的SOD酶活性和叶绿素含量明显高于对照。W6基因既能诱导抗病相关的PR基因表达又能诱导与提高非生物胁迫能力的COR/RD基因表达, 可能与生物胁迫和非生物胁迫相关, 证实转录因子W6参与了耐盐胁迫相关基因的调节, W6基因的过表达提高了转基因烟草的耐盐性。

关键词: ERF, 转基因, 耐盐性, 烟草

Abstract: Ethylene responsive factors (ERFs) are important plant-specific transcription factors. Some of them have been identified to interact with the ethylene-responsive GCC box and the dehydration responsive element (DRE). They usually play very important regulatory roles in response to biotic and abiotic stresses in plants. In our study, an ERF gene W6 was isolated from wheat (Xiao baimai) cDNA library, which belongs to ERF IV subfamily. Full-length W6 cDNA encoded an ERF protein containing a conserved ERF DNA-binding motif, two putative nuclear localization sequences and a C-terminal acidic transcription activation domain. W6 expression in wheat can be induced by various treatments such as cold, drought, salt, ABA and fungal pathogens. We constructed transgenic vector (35S::pBI121::W6) containing CaMV 35S promoter and then obtained W6-overexpresed tobacco plants by Agrobacterium-mediated transformation in order to identify the function of W6. Under the treatment with 200 mmol L-1 NaCl for 50 d, the transgenic plants grew well but the control plants nearly died. The root length of transgenic tobacco plants was between 1.40 cm and 3.93 cm, while that of the control only 0.20 cm. The root weight of transgenic tobacco plants was between 2.41 g and 7.79 g, while that of the control only 0.06 g. SOD activity and chlorophyll content in transgenic plants increased markedly compared to the control. All results the above showed that the overexpression of W6 improved tobacco’s salt tolerance, and W6 may act as a connector among different signal transduction pathways. The overexpression of W6 activated the expression of GCC box-containing genes PR2, PR3, PR5, and DRE/CRT genes NtERD10A and NtERD10C under normal growth conditions, and the tolerance to salt stress was improved in transgenic tobacco plants, which suggested that W6 regulates osmotic tolerance by activation of downstream gene expression through interaction with the GCC box or DRE.

Key words: ERF, Transgenic plants, Salt tolerance, Tobacco

[1] 周文期, 强晓霞, 王森, 江静雯, 卫万荣. 水稻OsLPL2/PIR基因抗旱耐盐机制研究[J]. 作物学报, 2022, 48(6): 1401-1415.
[2] 单露英, 李俊, 李亮, 张丽, 王颢潜, 高佳琪, 吴刚, 武玉花, 张秀杰. 转基因玉米NK603基体标准物质研制[J]. 作物学报, 2022, 48(5): 1059-1070.
[3] 陈悦, 孙明哲, 贾博为, 冷月, 孙晓丽. 水稻AP2/ERF转录因子参与逆境胁迫应答的分子机制研究进展[J]. 作物学报, 2022, 48(4): 781-790.
[4] 胡亮亮, 王素华, 王丽侠, 程须珍, 陈红霖. 绿豆种质资源苗期耐盐性鉴定及耐盐种质筛选[J]. 作物学报, 2022, 48(2): 367-379.
[5] 王渭霞, 赖凤香, 胡海燕, 何佳春, 魏琪, 万品俊, 傅强. 超低温11年保存期对转基因作物基体标准样品核酸检测的影响[J]. 作物学报, 2022, 48(1): 238-248.
[6] 李杰华, 端群, 史明涛, 吴潞梅, 柳寒, 林拥军, 吴高兵, 范楚川, 周永明. 新型抗广谱性除草剂草甘膦转基因油菜的创制及其鉴定[J]. 作物学报, 2021, 47(5): 789-798.
[7] 蒙姜宇, 梁光伟, 贺亚军, 钱伟. 甘蓝型油菜耐盐和耐旱相关性状的QTL分析[J]. 作物学报, 2021, 47(3): 462-471.
[8] 李鹏, 刘彻, 宋皓, 姚盼盼, 苏沛霖, 魏跃伟, 杨永霞, 李青常. 烟草非特异性脂质转移蛋白基因家族的鉴定与分析[J]. 作物学报, 2021, 47(11): 2184-2198.
[9] 杨琴莉, 杨多凤, 丁林云, 赵汀, 张军, 梅欢, 黄楚珺, 高阳, 叶莉, 高梦涛, 严孙艺, 张天真, 胡艳. 棉花花器官突变体的鉴定及候选基因的克隆[J]. 作物学报, 2021, 47(10): 1854-1862.
[10] 李健, 王逸茹, 张凌霄, 孙明昊, 秦阳, 郑军. 玉米ZmCIPK24-2基因在盐胁迫应答中的功能研究[J]. 作物学报, 2020, 46(9): 1351-1358.
[11] 董庆园,马德清,杨学,刘勇,黄昌军,袁诚,方敦煌,于海芹,童治军,沈俊儒,许银莲,罗美中,李永平,曾建敏. 高抗黑胫病烤烟BAC文库的构建及分析[J]. 作物学报, 2020, 46(6): 869-877.
[12] 宝力格,陆平,史梦莎,许月,刘敏轩. 中国高粱地方种质芽期苗期耐盐性筛选及鉴定[J]. 作物学报, 2020, 46(5): 734-744.
[13] 李俊,李亮,李夏莹,宋贵文,沈平,张丽,翟杉杉,柳方方,吴刚,张秀杰,武玉花. 转基因玉米MIR604基体标准物质研制[J]. 作物学报, 2020, 46(4): 473-483.
[14] 衡友强,游西龙,王艳. 费尔干猪毛菜病程相关蛋白SfPR1a基因的异源表达增强了烟草对干旱、盐及叶斑病的抗性[J]. 作物学报, 2020, 46(4): 503-512.
[15] 陈杉彬, 孙思凡, 聂楠, 杜冰, 何绍贞, 刘庆昌, 翟红. 甘薯IbCAF1基因的克隆及耐盐性、抗旱性鉴定[J]. 作物学报, 2020, 46(12): 1862-1869.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!