关键词: 水稻, 诱变, γ射线, EMS（甲基磺酸乙酯）, 突变体

Abstract:

Rice has become an established model system in the last decade and the organism chosen in the study of the cereal genomes. In order to find more mutants in the same genetic background, the seeds of indica rice cultivar 9311 were treated with γ-rays or EMS (ethyl methan sulfonate). Some mutants with the variations of leaf, stalk, panicle or root traits were found in the progenies, which the mutation frequency was 5.62%. Four hundred and sixty-five out of the 675 mutants were induced by γ-rays, and the other 210 mutants by EMS. Comparing with the EMS mutagenesis population, more mutants were produced in γ-rays mutagenesis population. But some mutants, such as the purple leaf sheath mutant and lesion resembling disease mutant, were only found in EMS mutagenesis population. The mutant library created will be helpful for gene identification and gene functional analysis.

Key words: Rice (Oryza sativa L.), Mutagenesis, γ-rays, EMS (Ethyl Methan Sulfonate), Mutant