甘蓝型油菜产量及其构成因素的QTL定位与分析

作物学报 ›› 2006, Vol. 32 ›› Issue (08): 1135-1142.

甘蓝型油菜产量及其构成因素的QTL定位与分析

张书芬^1,2;傅廷栋¹;朱家成²;王建平²;文雁成²;马朝芝¹

1 华中农业大学作物遗传改良国家重点实验室，湖北武汉 430070；2 河南省农业科学院棉花油料作物研究所，河南郑州 450002

收稿日期:2005-08-25 修回日期:1900-01-01 出版日期:2006-08-12 网络出版日期:2006-08-12
通讯作者: 马朝芝

QTL Mapping and Epistasis Analysis for Yield and Its Components in Brassica napus L.

ZHANG Shu-Fen^{1 2},FU Ting-Dong¹,ZHU Jia-Cheng²,WANG Jian-Ping²,WEN Yan-Cheng²,MA Chao-Zhi¹

1National Key Laboratory of Crop Genetic Improvement，Huazhong Agricultural University，Wuhan 430070, Hubei; 2Cotton and Oil Crops Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China

Received:2005-08-25 Revised:1900-01-01 Published:2006-08-12 Published online:2006-08-12
Contact: MA Chao-Zhi

摘要/Abstract

摘要：

产量性状是复杂的数量性状, 对种子的单株产量及其构成因素(全株总有效角果数、每角粒数、千粒重)进行QTL定位和上位性分析，确定其在染色体上的位置及其遗传效应,可以探讨油菜杂种优势产生原因，提高育种中对产量性状优良基因型选择的效率，达到提高油菜产量的目的。在双低油菜细胞质雄性不育保持系1141B和双高恢复系垦C₁构建的F₂作图群体中，运用SRAP、AFLP和SSR三种标记技术构建了一个甘蓝型油菜(Brassica napus L.)的分子标记遗传连锁图谱。共包含244个标记，分布于20个主要连锁群、1个三联体上，图谱总长度为2 769.5 cM。采用Windows QTL Cartographer Version 2.0统计软件及复合区间作图法，对油菜单株产量及其3大构成因素进行QTL定位，共检测到QTLs 16个分布在9个连锁群上，其中第6和13连锁群最多，均有3个。单个QTL解释性状表型变异的0.38%～73.34%。对于同一性状，等位基因的增效作用既来自母本，亦源自父本；采用双向方差分析法对位点间互作及其上位性进行分析，检测到26对影响产量构成性状的上位性互作效应QTL，说明油菜基因组中存在大量控制产量的互作位点，油菜产量性状的上位性存在着多效性，上位性互作包括QTL与非QTL位点，其中以非QTL位点较多。一般互作位点的独立效应值较小，而互作的效应值显著增大，且一般超过两位点独立效应值之和。反映了控制产量性状基因的复杂性。上位性是甘蓝型油菜产量性状杂种优势的重要遗传基础。

关键词: 甘蓝型油菜, 产量构成因素, 遗传图谱, 分子标记, QTLs定位, 上位性

Abstract:

Since yield traits are complicated quantitative traits, QTLs mapping and genetic effects analyzed for seed yield and its components can help us to understand the source for heterosis and improve the selecting efficiency for good genotype of seed yield. A genetic linkage map consisting of 244 DNA markers was constructed based on F₂ population derived from a cross between double low parent CMS maintainer 1141B and double high CMS restorer KenC1. The markers in the linkage map distributed on all the 20 main linkage groups and 1 triplet and covered 2 769.5 cM of the rapeseed genome. The statistic software of Windows QTL Cartographer Version2.0 and Composite Internal Mapping (CIM) were applied to detect QTLs for seed yield and its components, including seed yield per plant, number of silique per plant, number of seed per silique and 1000-seed weight. A total of 16 QTLs located in nine different linkage groups were identified for seed yield and the three component traits. There were three QTLs (the most QTLs) in LG6 and LG13, respectively. Each of these QTLs explained 0.38%–73.34% of the phenotypic variance. They had additional effect of alleles that came from maternal parent, the others from paternal parent for the same trait. Epistatic effect was screened in all 20 linkage groups using all possible loci pairs by two-way ANOVAs between co-dominant markers. Twenty-six two-locus combinations had significant epistatic effects. The results showed that there were many interaction loci involving the entire genome detected in yield traits. The epistatic interactions had pleiotropic effects, interactions were contained epistasis between QTL and non-QTL (non-significant effect loci) and between non-QTL. The later was the majority. In general, the independent effect of interaction loci was lower, on the opposite, the value of interaction effect was higher, and much higher than the total of independent effects.This indicated that the genes controlling seed yield was complicated. Epistasis interactions might play an important role as the basis of heterosis in Brassica napus L. Some questions about QTLs mapping were also discussed.

Key words: Brassica napus L., Yield components, Genetic linkage map, Molecular marker, QTL mapping, Epistatic effect

中图分类号:

Q943

张书芬;傅廷栋;朱家成;王建平;文雁成;马朝芝. 甘蓝型油菜产量及其构成因素的QTL定位与分析[J]. 作物学报, 2006, 32(08): 1135-1142.

ZHANG Shu-Fen ;FU Ting-Dong;ZHU Jia-Cheng;WANG Jian-Ping;WEN Yan-Cheng;MA Chao-Zhi. QTL Mapping and Epistasis Analysis for Yield and Its Components in Brassica napus L.[J]. Acta Agron Sin, 2006, 32(08): 1135-1142.

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链接本文: https://zwxb.chinacrops.org/CN/

https://zwxb.chinacrops.org/CN/Y2006/V32/I08/1135

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