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作物学报 ›› 2010, Vol. 36 ›› Issue (2): 217-227.doi: 10.3724/SP.J.1006.2010.00217

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

小麦旗叶叶绿素含量及荧光动力学参数与产量的灰色关联度分析

王正航1，武仙山2，昌小平2，李润植1，景蕊莲2,*   

  1. 1山西农业大学，山西太谷 030801；2国家基因资源与遗传改良重大科学工程 / 农业部作物种质资源利用重点实验室 / 中国农业科学院作物科学研究所，北京 100081
  • 收稿日期:2009-07-27 修回日期:2009-12-08 出版日期:2010-02-10 网络出版日期:2009-12-21
  • 通讯作者: 景蕊莲, E-mail: jingrl@caas.net.cn, Tel: 010-82105829
  • 基金资助:

    本研究由国家高技术研究发展计划（863计划）项目（2006AA100201）和国家转基因生物新品种培育重大专项（2008ZX08002-002）资助。

Chlorophyll Content and Chlorophyll Fluorescence Kinetics Parameters of Flag Leaf and Their Gray Relational Grade with Yield in Wheat

WANG Zheng-Hang1,WU Xian-Shan2,CHANG Xiao-Ping2,LI Run-Zhi1,JING Rui-Lian2*   

  1. 1 Shanxi Agricultural University, Taigu 030801, China; 2 National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, china
  • Received:2009-07-27 Revised:2009-12-08 Published:2010-02-10 Published online:2009-12-21
  • Contact: JING Rui-Lian, E-mail: jingrl@caas.net.cn, Tel: 010-82105829

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被引次数

134

摘要：

为探讨干旱胁迫条件下小麦旗叶光合性状的变化及其对产量的影响，以小麦RIL群体(旱选10号×鲁麦14) F8代的305个株系及其亲本为材料，分析雨养和灌溉两种水分条件下，开花期和灌浆期旗叶叶绿素含量和叶绿素荧光动力学参数的变化情况、相关关系、遗传力、基因数目以及不同性状与产量的关系。结果表明，在两种水分条件下，RIL群体性状多数表现超亲现象，变异系数为1.12%~67.05%。雨养条件下抗旱亲本旱选10号的旗叶叶绿素含量及叶绿素荧光动力学参数比水地品种鲁麦14表现稳定。两种水分条件下，两个生育时期的旗叶叶绿素含量极显著正相关，而荧光动力学参数相关性表现复杂，Fm与Fv、Fo/Fm与Fv/Fm之间相关性最高，相关系数大于0.994。在所有性状中，叶绿素含量的遗传力最高；多数性状在雨养条件下的基因数目多于灌溉条件的，控制灌浆期Fv/Fo的基因数目最多，达34对。灰色关联度分析显示，灌浆期Fm、Fv及Fo与Fm曲线之间的面积对产量影响较大，可以作为选择抗旱高光效小麦的重要评价指标。

关键词: 小麦, RIL群体, 干旱胁迫, 叶绿素含量, 叶绿素荧光动力学参数, 产量, 灰色关联度

Abstract:

Drought stress impacts photosynthetic characteristics and results in a diminished output in wheat (Triticum aestivum L.). In the variety screening and breeding of wheat for high photosynthetic efficiency and drought resistance, indices for the assessment are of great importance. Although a few investigators have studied the physiological mechanism of photosynthesis on the basis of the chlorophyll fluorescence kinetics parameters with several wheat varieties, the relationships between these parameters and grain yield were not completely clear. The inheritance of chlorophyll related traits under different water conditions has not been reported, especially using genetic populations.To dissect the dynamics of photosynthetic characteristics and the heritabilities of chlorophyll content and chlorophyll fluorescence kinetics parameters in wheat, the authors have constructed a set of recombinant inbred lines (RILs) through crossing a highly drought-resistant variety, Hanxuan 10, and a high-yielding variety Lumai 14 grown in irrigated areas. In this study, 305 RILs of F8 generation were evaluated in well-watered and rainfed (drought stress) environments, and traits of chlorophyll content and 7 chlorophyll fluorescence kinetics parameters in flag leaves were measured at flowering and filling stages. Most of the mean values of traits showed substantial transgressive segregation in the RILs, and the variation coefficients ranged from 1.12% to 67.05% under both water regimes. All traits measurements except for Fo and Fo/Fm in the RILs and their parents were lower under rainfed condition than under the well-watered condition.The chlorophyll content andthe chlorophyll fluorescence kinetics parameters were more stable in Hanxuan 10 than in Lumai 14. Significantly positive correlations were observed in chlorophyll content between either water conditions or growth stages, and the correlation coefficients ranged from 0.499 (P < 0.01) to 0.717 (P < 0.01). However, correlations among the chlorophyll fluorescence kinetics parameters were complex, of which the largest correlation coefficients (more than 0.994) were observed between Fm and Fv and between Fo/Fm and Fv/Fm. Among all the traits tested, chlorophyll content had the highest heritability that was no less than 0.81 in both treatments. According to the primary estimates, most traits under rainfed condition were controlled by more genes than under well-watered condition. For instance, the maximum genes, totally 34, were detected for Fv/Fo at filling stage under rainfed condition; 33 genes were detected for Fv at filling stage under rainfed condition and for Fv/Fm and Fo/Fm at flowering stage under rainfed condition; 32 genes were found for Fm at filling stage under rainfed condition. The gray relational grade analysis indicated that Fv, Fm, and the area between curves of Fo and Fm at filling stage made important impacts on the grain yield. Therefore, they are considered as important indices for in the selection of drought tolerance and high photosynthetic efficiency in wheat.

Key words: Wheat, Recombinant inbred lines, Drought stress, Chlorophyll content, Chlorophyll fluorescence kinetics parameters, Yield, Gray relational grade

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