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作物学报 ›› 2010, Vol. 36 ›› Issue (2): 233-241.doi: 10.3724/SP.J.1006.2010.00233

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

大豆[Glycine max（L.）]成熟期近等基因系导入片段分析

魏金鹏1,**，鄂文弟1,2,**，刘章雄1，关荣霞1，常汝镇1，邱丽娟1,*   

  1. 1 中国农业科学院作物科学研究所 / 国家农作物基因资源与遗传改良重大科学工程 / 农业部作物种质资源利用重点开放实验室, 北京100081; 2 全国农业技术服务推广中心, 北京 100026
  • 收稿日期:2009-05-26 修回日期:2009-10-02 出版日期:2010-02-10 网络出版日期:2009-12-21
  • 通讯作者: 邱丽娟,E-mail:qiu_lijuan@263.net;Tel:010-82105843
  • 基金资助:

    本研究由国家高技术研究发展计划（863计划）（2006AA100104，2006AA10A11D，2006AA10Z1F1，2006AA10Z1B3）和国家科技支撑项目（2006BAD13B05）资助。

Analysis of Introgressed Segments in Near-Isogenic Lines Carrying Soybean Maturity Genes

WEI Jin-Peng1,**,E Wen-Di2,**,LIU Zhang-Xiong1,GUAN Rong-Xia1,CHANG Ru-Zhen1,QIU Li-Juan1*   

  1. 1 National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Crop Germplasm Utilization, Ministry of Agriculture / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 China Agricultural Technology Extension Service Center, Beijing 100026, China
  • Received:2009-05-26 Revised:2009-10-02 Published:2010-02-10 Published online:2009-12-21
  • Contact: QIU Li-Juan,E-mail: qiu_lijuan@263.net;Tel:010-82105843

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被引次数

6

摘要：

选择243个多态性SSR标记, 分析轮回亲本Harosoy及23个携带大豆4个不同成熟期基因的近等基因系(near isogenic line, NIL), 共检测出导入片段266个, 平均每个NIL有导入片段11.6个。其中由携带E1基因的NIL检测出导入片段150个, 主要集中在第6号染色体；由携带E2、e3和E5基因的NIL各检测出导入片段55个、49个和73个, 分别集中在第20号、第12号和第20号染色体, 根据NIL的SSR分析结果聚类，具有相同熟期基因的NILs趋向聚在一起。通过对导入片段进行分析, 推测E1基因与第6号染色体的satt643~sat_312区间及第11号染色体的sat_095位点相关, E2和E5基因与第20号染色体的satt587~satt496区间相关, e3与第12号染色体的satt317~satt181区间相关。结果表明, 利用近等基因系不仅验证了已知E1基因所在的染色体区间, 发现了一个新的标记位点与E1相关, 还鉴定出与E2、e3和E5基因相关的标记, 明确了轮回亲本中成熟期基因所在导入片段大小及其位置, 为成熟期基因的精细定位和克隆提供了信息。

关键词: 大豆, 近等基因系, 导入片段, 成熟期

Abstract:

It is very important to exploit the soybean maturity genes for developing varieties with high-yield and wide adaptability in both theory and practice. The objective of the study was to identify the size and position of introgressed segments associating with maturity genes, which provides information of fine mapping and cloning maturity genes. Twenty-three introgression lines (near isogenic lines, NILs) carrying four soybean maturity genes and their recurrent parent, were analyzed with 243 SSR markers, and 266 introgression segments were found, so each NIL containing 11.6 introgressed segments on average. The majority of 150 introgressed segments with E1, were located on chromosome 6, while 55, 49; 73 introgressed segments with E2, e3, E5on chromosomes 20, 12 and 20 respectively. The NILs with the same maturity gene trended to cluster together. The results showed that E1 was related to the interval of satt643–sat_312 on chromosome 6 and sat_095 on chromosome 11; both E2 and E5 loci associated with the interval of satt587–satt496 on chromosome 20; e3 was related to the interval of satt317–satt181 on chromosome 12. Therefore, E1 locus was validated with NILs, anda new markerrelated to E1 was detected, and E2, e3, E5 gene-related markers were identified.

Key words: Soybenan, Near-isogenic lines, Introgressed segments, Maturity


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