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作物学报 ›› 2010, Vol. 36 ›› Issue (2): 242-248.doi: 10.3724/SP.J.1006.2010.00242

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

大豆叶绿素含量动态表达的QTL分析

李广军1,2,李河南1,程利国1,章元明1,*   

  1. 1 南京农业大学作物遗传与种质创新国家重点实验室 / 国家大豆改良中心，江苏南京 210095；2 临沂师范学院，山东临沂 276005
  • 收稿日期:2009-07-28 修回日期:2009-12-08 出版日期:2010-02-10 网络出版日期:2009-12-21
  • 通讯作者: 章元明，E-mail: soyzhang@njau.edu.cn
  • 基金资助:

    本研究由国家自然科学基金项目（30470998），教育部新世纪优秀人才支持计划项目（NECT-05-0489），高等学校博士点基金项目（20060307008），江苏省自然科学基金项目（BK2008335）和教育部"111"计划（B08025）资助。

QTL Analysis for Dynamic Expression of Chlorophyll Content in Soybean (Glycine max L. Merri.)

LI Guang-Jun1,2,LI He-Nan,CHENG Li-Guo,ZHANG Yuan-Ming1,*   

  1. 1 State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University / National Center for Soybean Improvement, Nanjing 210095, China; 2 Linyi Normal University, Linyi 276005, China
  • Received:2009-07-28 Revised:2009-12-08 Published:2010-02-10 Published online:2009-12-21
  • Contact: ZHANG Yuan-Ming，E-mail:soyzhang@njau.edu.cn

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1599

被引次数

38

摘要：

叶绿素是光合作用中最重要的色素，与大豆籽粒产量密切相关。本研究采用溧水中子黄豆×南农493-1后代衍生的244个F2单株，及筛选的150个SSR分子标记构建的连锁遗传图谱，在苗期至开花期测定F2衍生F2:3和F2:4家系生长正常单株的倒3复叶功能叶(非离体)的叶绿素含量13次，通过Windows QTL Cartographer v2.5软件包的复合区间法，动态定位了大豆叶绿素含量的QTL。结果表明，不同时间点共检测到20个QTL，其中，不同发育阶段间、年份间和地点间共同的QTL较少，不同时间点上的QTL差异较大，重复出现在N、D1a、F和K连锁群的QTL有3~4次。这些结果为叶绿素含量的遗传剖析和标记辅助育种提供理论依据。

关键词: 大豆, 叶绿素含量, QTL, 动态表达

Abstract:

Chlorophyll is the most important photosynthetic pigment and closely related to soybean seed yield. However, there are still very few studies at different developmental stages under multiple environments. A genetic linkage map using 244 F2 plants derived from a soybean cross between Lishuizhongzihuang and Nannong 493-1 was constructed. F2:3 and F2:4 families were used to dissect the developmental behavior for chlorophyll content across different environments by quantitative trait locus (QTL) mapping approach. Chlorophyll contentsfor the function leaf (in vivo) in the 244 F2:3 and F2:4 families from seedling to blooming stages at Jiangpu and/or Linyi experimental stations in 2007 and/or 2008 were measured by SPAD-502 chlorophyll instrument. Composite interval mapping (CIM) of Windows QTL Cartographer V2.5 was used for the QTL analysis. A total of 20 QTLs were detected at various developmental stages under two environments, but there were few common QTLs identified across different developmental stages, across years and across experimental stations, although there were 3 to 4 QTLs detected on each of the N, D1a, F and K linkage groups. In the two years above, one common QTL, qchl-D1a-1, located between markers sat_160 and satt147 on the linkage group D1a, was identified at Jiangpu experimental station. At the two experimental stations above, three common QTLs on the K, M and N linkage groups were mapped. These results provide a theoretical basis for genetic analysis of chlorophyll traits and marker-assisted breeding.

Key words: Soybean, Chlorphyll, Quantitative trait locus, Dynamic Expression


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