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作物学报 ›› 2010, Vol. 36 ›› Issue (2): 267-275.doi: 10.3724/SP.J.1006.2010.00267

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

小麦苗期光合作用及其相关性状的QTL分析

梁燕1,张坤普2,赵亮1,梁雪1,张雯婷1,孙晓琳1,孟庆伟1,田纪春1,*,赵世杰1,*   

  1. 1山东农业大学国家作物生物学重点实验室, 山东泰安 271018；2 中国科学院遗传与发育生物学研究所，北京 100101
  • 收稿日期:2009-05-26 修回日期:2009-10-02 出版日期:2010-02-10 网络出版日期:2010-01-10
  • 通讯作者: 赵世杰, E-mail: sjzhao@sdau.edu.cn; Tel: 0538-8249767; 田纪春, E-mail: jctian@sdau.edu.cn; Tel: 0538-8242040
  • 基金资助:

    本研究由国家重点基础研究发展计划（973计划）项目（2009CB118301）和国家高技术研究发展计划（863计划）项目（2006AA100101）资助。

Analysis of QTLs Associated with Photosynthesis Characteristics in Wheat Seedlings

LIANG Yan1,ZHANG Kun-Pu2,ZHAO Liang1,LIANG Xue1,ZHANG Wen-Ting1,SUN Xiao-Lin1,MENG Qing-Wei1,TIAN Ji-Chun1,*,ZHAO Shi-Jie1,*
  

  1. 1 State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, China; 2Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
  • Received:2009-05-26 Revised:2009-10-02 Published:2010-02-10 Published online:2010-01-10
  • Contact: ZHAO Shi-Jie, E-mail: sjzhao@sdau.edu.cn; Tel: 0538-8249767;WANG Ji-Chun, E-mail: jctian@sdau.edu.cn; Tel: 0538-8242040

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1360

被引次数

20

摘要：

将小麦品种花培3号和豫麦57构建的DH群体的168个株系及其亲本，盆栽于两个环境中，利用324个SSR标记位点构建遗传图谱，对单叶净光合速率及相关参数、叶绿体色素含量和叶绿素荧光参数进行QTL定位和分析。利用基于混合线性模型的QTLNetwork 2.0，共检测到17个加性效应和20对上位性效应位点，其中所有加性效应位点和16对上位性效应位点具有环境互作效应。相关性较高的性状间有一些共同的QTL，表现出一因多效或者紧密连锁效应。在5D染色体上的Xwmc215至Xgdm63区段，检测到控制叶绿素a、叶绿素b和类胡萝卜素含量的3个主效QTL，各位点的遗传效应贡献率较大，增效基因均来源于花培3号，适用于分子标记辅助选择和聚合育种。另外，该区段与控制单叶净光合速率(Pn)、气孔导度(Gs)、胞间CO2浓度(Ci)和胞间CO2浓度与胞外CO2浓度比值(Ci/Cr的QTL的定位区间相近。位于5B染色体控制胞间CO2浓度的QTL是个微效基因，但是QTL与两种环境的互作效应表现的遗传贡献比较大。)

关键词: 净光合速率, 叶绿素含量, 叶绿素荧光参数, QTL定位, 小麦

Abstract:

For the purpose of detecting QTLs associated with photosynthetic related traits, a set of 168 doubled haploid (DH) lines derived from the cross between Huapei 3 and Yumai 57 was tested with 324 SSR markers covering the whole genome of wheat (Triticum aestivum L.). The net photosynthetic rate, gas changes, chlorophyll content, and chlorophyll fluorescence in leaves were investigated in both DH population and the parents at seedling stage. QTL analysis was carried out using QTLNetwork version 2.0 based on the mixed linear model. A total of 17 additive QTLs and 20 pairs of epistatic QTLs were detected for the photosynthetic related traits. All additive QTLs and 16 pairs of epistatic QTLs had interactions with environments. In agreement with the high correlations of phenotypes, several traits shared common QTL regions, and showed tight linkages of these QTLs or pleiotropisms. In the interval between Xwmc215 and Xgdw63 on chromosome 5D, three major additive QTLs for chlorophyll a, chlorophyll b, and carotinoid contents explained the phenotypic variations by 18.23%, 10.40%, and 27.25%, respectively, whose positive alleles were all originated from Huapei 3. These QTLs are favorable for marker-assisted selection. In addition, this region was near the QTLs for net photosynthetic rate (Pn), stomatal conductance (Gs), intercellular CO2 concentration (Ci), and the ratio of Cito gas conductance (Cr). The QTL for Ci on chromosome 5B was a minor locus but explained relatively great phenotypic variation in the interactions between QTLs and environments.

Key words: Photosynthetic rate, Chlorophyll content, Chlorophyll fluorescence parameters, QTL mapping, Wheat

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