欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2010, Vol. 36 ›› Issue (4): 665-672.doi: 10.3724/SP.J.1006.2010.00665

• 耕作栽培·生理生化 • 上一篇    下一篇

喷施硼肥对紫花苜蓿光合作用及可溶性糖源库间运转的影响

宗毓铮,王雯玥,韩清芳*,丁瑞霞,贾志宽,聂俊峰   

  1. 西北农林科技大学干旱半干旱研究中心/农业部旱地作物生产与生态重点开放实验室,陕西杨凌712100
  • 收稿日期:2009-08-12 修回日期:2009-12-18 出版日期:2010-04-12 网络出版日期:2010-03-03
  • 通讯作者: 韩清芳, E-mail: hanqf88@126.com
  • 基金资助:

    本研究由陕西省自然科学基金项目(2006C105),国家"十一五"旱地农业支撑计划课题(2006BAD29B03)和西北农林科技大学唐仲英育种基金(05YZ019)项目资助.

Effects of Different Levels of Boron Fertilizer on Alfalfa Photosynthesis and Source-Sink Translocation of Soluble Carbohydrate

ZONG Yu-Zheng,WANG Wen-Yue,HAN Qing-Fang*,DING Rui-Xia,JIA Zhi-Kuan,NIE Jun-Feng   

  1. The Arid and Semi-arid Areas Research Center of Agriculture Northwest A&F University/Key Laboratory of Crop Production and ecology,Minister of Agriculture,the People's Republic of China,Yangling 712100,China
  • Received:2009-08-12 Revised:2009-12-18 Published:2010-04-12 Published online:2010-03-03
  • Contact: HAN Qing-Fang,E-mail:hanqf88@126.com

PDF

1444

被引次数

48

摘要：

通过叶面喷施不同次数、不同浓度硼肥，研究其对缺硼土壤条件下紫花苜蓿光合作用及可溶性糖运转的影响，探讨苜蓿吸收的硼规律及高产施硼技术。结果表明，从返青期开始，连续在分枝期和现蕾初期累计喷施1次、2次或3次5个浓度的硼砂，均不同程度影响紫花苜蓿光合特性和可溶性糖源库间的运转。喷施3次0.3%(W/W)的硼砂对提高叶片中B含量、光合速率(Pn)、蒸腾速率(Tr)和叶绿素含量(Chl)作用最显著，较对照提高幅度分别为75%、39%、30%和70%，喷施3次0.2%的硼砂对增大叶面积效果最佳，较对照增加37%，并可有效降低叶片可溶性糖含量(50%)；一次或多次喷施0.5%(W/W)的硼砂会导致硼中毒现象，抑制光合作用，植株生长受阻。

关键词: 紫花苜蓿, 光合作用, 叶绿素含量, 可溶性糖

Abstract:

Alfalfa, one of the uppermost leguminous grasses in the world, is widely cultivated in arid-semiarid areas of the loess plateau. Because the content of water-soluble Boron (B) is less than 0.5 mg kg–1 in 56% soil in loess plateau and alfalfa is a highly sensitive crop to B, it is necessary to supply adequate B for perennial alfalfa growth. Previous studies showed that physiological characteristics of crops were influenced by B through its effect on the structure of chloroplast, promoting the transportation of sugar and adjusting the balance between moisture and nutrient, then indirectly on the oxidation and reduction process of photosynthesis and respiration of crops. Until now, most studies have concentrated on the effects of B on yield and quality of alfalfa, few reports on the impact of B on photosynthetic characteristics of alfalfa were available. This study focused on the effects of different B fertilizer levels on the photosynthesis and translocation of soluble carbohydrate by spraying B on the leaves surface of alfalfa grown in B deficient soil, and investigated the high-yield technology of applying B fertilizer in alfalfa. The results showed that photosynthesis and translocation of soluble carbohydrate between source and sink were differently influenced by spraying five concentrations of B fertilizer with different times (1, 2, or 3 times) at reviving, branching and early flower bud stages. Significant influences were observed on the increase of Pn, Tr, and chlorophyll content by 39%, 30%, and 70%, respectively, in the treatment of spaying three times of 0.3% borax solution compared with the control. Leaf area increased by 37% and soluble carbohydrate concentration in leaves decreased by 50% in the treatment of spraying three times of 0.2% borax solution. B content, Pn, and Tr in leaf were significantly increased by 51%, 33%, and 25%, respectively in the treatment of spraying two times of 0.4% borax solution compared with the control. However, B intoxication might occur, along with the decrease of photosynthesis capacity and retardness of growth by spraying 0.5% borax solution 1–3 times.

Key words: Boron, Alfalfa, Photosynthesis, Chlorophyll content, Soluble carbohydrate

[1] Zhu X-M(朱显谟). Soil and Agriculture on Loess Plateau (黄土高原土壤与农业). Beijing: Agriculture Press, 1989. pp 310-315 (in Chinese)

[2] Wang Q(王琴), Han Q-F(韩清芳), Jia Z-K(贾志宽). Effect of spraying trace element fertilizers at different levels on alfalfa quality in arid and semiarid areas of northwestern China. Acta Agrest Sin草地学报), 2008, 16(4): 408-411 (in Chinese with English abstract) (

[3] O’Neill MA, Ishii T, Albersheim P, Darvill A G. Rhamnogalacturonan: II. Structure and function of a borate cross-linked cell wall pectic polysaccharide. Annu Rev Plant Biol, 2004, 55: 109-139

[4] Liu P(刘鹏), Yang Y-A(杨玉爱). Effects of molybdenum and boron on membrane lipid peroxidation and endogenous protective systems of soybean leaves. Acta Bot Sin植物学报), 2000, 42(5): 461-466 (in Chinese with English abstract) (

[5] Match T. Boron in plant cell walls. Plant Soil, 1997, 193: 59-70

[6] Iwai H, Hokura A, Oishi M, Chida H, Ishii T, Sakai S, Satoh S. The gene responsible for borate cross-linking of pection rhamnogalacturonan-II is required for plant reproductive tissue development and fertilization. Proc Natl Acad Sci USA, 2006, 103: 16592-16597

[7] Guo Y-H(郭玉虹). Effects of trace element fertilizer in soybean production. Soybean Bull (大豆通报), 1995, (3): 27 (in Chinese)

[8] Zhang J-C(张继澍). Plant Physiology (植物生理学). Beijing: Higher Education Press, 2006. pp 95-100 (in Chinese)

[9] Zu Y-Q(祖艳群), Lin K-H(林克惠). The role of boron in plants and its effect on the yield and quality of crops. J Yunnan Agric Univ (云南农业大学学报), 2000, 12(4): 359-363 (in Chinese with English abstract)

[10] Liu P(刘鹏). Research on effects of Molybdenum and Boron to the Quality and Physiological Mechanism of Soybean. PhD Dissertation of Zhejiang University, 2000 (in Chinese with English abstract)

[11] Liu G-H(刘贵河), Han J-G(韩建国), Wang K(王堃). Effects of boron, molybdenum, zinc and macronutrients on the yield and quality of alfalfa. Acta Agrest Sin草地学报), 2004, 12(4): 268-272 (in Chinese with English abstract) (

[12] Hu H-F(胡华锋), Jie X-L(介晓磊), Guo X(郭孝). Effect of spraying trace elements on nutrient quality of Medicago sativa. Chin J Grassland中国草地学报), 2007, 29(2): 107-111 (in Chinese with English abstract) (

[13] Wang Y(王云), Wei F-S(魏复盛), Yang G-Z(杨国治). Elements Chemistry in Soil Environment (土壤环境元素化学). Beijing: China Environmental Science Press, 1995. pp 289-318 (in Chinese)

[14] Guo Z-H(郭志华), Zhang X-D(张旭东), Huang L-L(黄玲玲). Solar energy and water utilization of Quercus mongolica, a deciduous broadleaf tree, in different light regimes across the edge of a deciduous broad-leaved forest. Acta Ecol Sin生态学报), 2006, 26(4): 1047-1056 (in Chinese with English abstract) (

[15] Bao S-D(鲍士旦). Agricultural Soil Analysis (土壤农化分析). Beijing: China Agricultural Press, 2000. pp 127-128, 267-277 (in Chinese)

[16] Gao J-F(高俊风). Experimental Guidance for Plant Physiology (植物生理学实验指导). Beijing: Higher Education Press, 2006. pp 144-147(in Chinese)

[17] Fischer R A, Turner N C. Plant productivity in the arid and semiarid zones. Annu Rev Plant Physiol, 1978, 29: 227-317

[18] Peng F-T(彭福田), Jiang Y-M(姜远茂), Gu M-R(顾曼如). Absorption and distribution of foliar applied 10B in apple trees during spring. Plant Physiol Commun植物生理学通讯), 2001, 37(5): 401-403 (in Chinese) (

[19] Tong Y-A(仝月澳), Zhou H-J(周厚基). Nutritional Diagnosis for Fruit Trees (果树营养诊断法). Beijing: Agriculture Press, 1982. pp 18-19, 102-104, 207-208 (in Chinese)

[20] Zhang Y-C(张宜春), Liu T-C(刘同仇), Xie Z-C(谢振翅), trans. Principles of Plant Nutrition (植物营养原理). Beijing: Agriculture Press, 1987. pp 550-561 (in Chinese)

[21] Zhong Y-Y(钟勇玉), Du J-B(杜军宝). Effect of soil lacking boron on the photosynthesis and breathing in mulberry leaf. Acta Agric Boreali-Occident Sin西北农业学报), 1996, 5(1): 58-62 (in Chinese with English abstract) (

[22] Lee S, Aronoff S. Boron in plants: A biochemical role. Science, 1967, 158: 798-799

[23] Liu Y-S(娄云生). Research on Absorption Effects of Boron to Different Rape Genotypes under Different Water and Fertilizer Conditions. PhD Dissertation of Zhejiang Agricultural University, 1998 (in Chinese with English abstract)

[24] Fang Y-H(方益华), Liu P(刘鹏). A review of advances in physiological function of boron in plants. Subtrop Plant Sci亚热带植物科学), 2002, 31(2): 64-69 (in Chinese with English abstract) (

[25] Sun Y-H(孙亚辉), Li R-Q(李瑞奇), Dang H-K(党红凯). Population and individual characteristics of growth and development and yield components of super-high-yielding winter wheat in Hebei province. J Agric Univ Hebei河北农业大学学报), 2007, 30(3): 1-8 (in Chinese with English abstract) (

[26] Wu L-S(吴礼树). The Influences of boron and potassium on nitrogenious compounds in cotton. J Huazhong Agric华中农业大学学报), 1987, (4): 336-340 (in Chinese with English abstract) (

[27] Xu G-D(徐根娣), Cai M-Z(蔡妙珍), Liu P(刘鹏). Effect of boron and manganese on photosynthetic characteristic of soybean. J Zhejiang Norm Univ浙江师范大学学报·自然科学版), 2004, 27(1): 62-65 (in Chinese with English abstract) (Nat Sci)(

[28] Niu Y(牛义), Zhang S-L(张盛林). The present conditions and prospects of plant boron nutrition. Chin Agric Sci Bull (中国农学通报), 2003, 19(2): 101-104 (in Chinese with English abstract)

[29] Milburn I A, Kallarackal J. Quantitative determination of sieve tube dimensions in Ricinus, Cucumis and Musa. New Phytog, 1994, 96: 383-395

[30] Liu P(刘鹏). The Research Development of Molybdenum & Boron Nutrition in Soybean. Chin Agric Sci Bull中国农学通报), 2001, 17(6): 41-44 (in Chinese with English abstract) (
[1] 周练, 刘朝显, 熊雨涵, 周京, 蔡一林. 质膜内在蛋白ZmPIP1;1参与玉米耐旱性和光合作用的功能分析[J]. 作物学报, 2021, 47(3): 472-480.
[2] 侯慧芝, 张绪成, 方彦杰, 于显枫, 王红丽, 马一凡, 张国平, 雷康宁. 全膜微垄沟播对寒旱区春小麦苗期土壤水热环境及光合作用的影响[J]. 作物学报, 2020, 46(9): 1398-1407.
[3] 荐红举, 霍强, 高玉敏, 李阳阳, 谢玲, 魏丽娟, 刘列钊, 卢坤, 李加纳. 用全基因组关联分析筛选甘蓝型油菜叶片叶绿素含量候选基因[J]. 作物学报, 2020, 46(10): 1557-1565.
[4] 于奇,冯乃杰,王诗雅,左官强,郑殿峰. S3307对始花期和始粒期淹水绿豆光合作用及产量的影响[J]. 作物学报, 2019, 45(7): 1080-1089.
[5] 吴含玉,肖飞,张亚黎,姜闯道,张旺锋. 强闪光抑制棉花叶片光系统II活性和热耗散[J]. 作物学报, 2019, 45(5): 792-797.
[6] 吴含玉,张雅君,张旺锋,王克如,李少昆,姜闯道. 田间密植诱导抽穗期玉米叶片衰老时的光合作用机制[J]. 作物学报, 2019, 45(2): 248-255.
[7] 李艳霞,杨卫兵,尹燕枰,郑孟静,陈金,杨东清,骆永丽,庞党伟,李勇,王振林. 小麦小穗不同粒位粒重形成的生理特性差异[J]. 作物学报, 2019, 45(11): 1715-1724.
[8] 肖飞,杨延龙,王娅婷,马慧,张旺锋. 棉花花铃期低温对叶片PSI和PSII光抑制的影响[J]. 作物学报, 2017, 43(09): 1401-1409.
[9] 王润青,樊晓聪,宋梅芳,肖阳,郭林,孟凡华,杨青华,吴大付,杨建平. 小麦DELLA获得性突变体矮变1号增强了幼苗的抗盐能力[J]. 作物学报, 2016, 42(11): 1721-1726.
[10] 刘凌云,刘浩,赵晶,王艳霞,王棚涛. 拟南芥低叶绿素荧光LCF3基因的克隆与功能分析[J]. 作物学报, 2016, 42(05): 690-695.
[11] 顾骏飞*,周振翔,李志康,戴琪星,孔祥胜,王志琴,杨建昌. 水稻低叶绿素含量突变对光合作用及产量的影响[J]. 作物学报, 2016, 42(04): 551-560.
[12] 梁慧珍,余永亮,杨红旗,董薇,许兰杰,牛永光,张海洋,刘学义,方宣钧. 大豆叶片性状和叶绿素含量QTL间的上位性和环境互作效应[J]. 作物学报, 2015, 41(06): 889-899.
[13] 向佳,李燕,樊亚伟,许军红,郑丽媛,何光华,杨正林,王楠,赵芳明*. 一个具有主效晚抽穗基因的水稻染色体片段代换系的鉴定、形态分析及Ehd4-2定位[J]. 作物学报, 2015, 41(05): 683-691.
[14] 王惠贞,赵洪亮,冯永祥,姜乐,宁大可,谢立勇,林而达. 北方水稻生育后期剑叶可溶性物质含量及植株生产力对CO2浓度增高的响应[J]. 作物学报, 2014, 40(02): 320-328.
[15] 晁毛妮,郝德荣,印志同,张晋玉,宋海娜,张怀仁,褚姗姗,张国正,喻德跃. 大豆生物量与产量组分间的相关及关联分析[J]. 作物学报, 2014, 40(01): 7-16.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2