欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2010, Vol. 36 ›› Issue (05): 833-839.doi: 10.3724/SP.J.1006.2010.00833

• 耕作栽培·生理生化 • 上一篇    下一篇

胞内钙库对小麦叶锈菌侵染之过敏反应的影响

张蓓,阎爱华,刘刚,刘猛,侯春燕,王冬梅*   

  1. 河北农业大学生命科学学院,河北保定071001
  • 收稿日期:2009-11-09 修回日期:2010-02-07 出版日期:2010-05-12 网络出版日期:2010-03-15
  • 通讯作者: 王冬梅, E-mail: dongmeiwang63@hotmail.com
  • 基金资助:

    本研究由国家自然科学基金(30671244),河北省应用基础研究计划重点基础研究项目(08965505D),河北省自然科学基金(303180,C2005000220,C2007000515)资助.

Effect of Intracellular Calcium Stores on Hypersensitive Reaction Induced by Wheat Leaf Rust Fungus

ZHANG Bei,YAN Ai-Hua,LIU Gang,LIU Meng,HOU Chun-Yan,WANG Dong-Mei*   

  1. College of Life Science,Agricultural University of Hebei,Baoding 071001,China
  • Received:2009-11-09 Revised:2010-02-07 Published:2010-05-12 Published online:2010-03-15
  • Contact: WANG Dong-Mei,E-mail:dongmeiwang63@hotmail.com

PDF

2174

被引次数

36

摘要：

使用影响胞内Ca2+库和钙通道的药物预注射小麦叶片，观察其对小麦受叶锈菌侵染诱发的过敏反应(HR)的影响。结果表明，对小麦叶片预注射不同浓度的胞内Ca2+螯合剂(BAPTA-AM)后接种叶锈菌小种260，随着注射药物浓度的增高，寄主细胞发生HR的面积逐渐减小。而注射胞内Ca2+激活剂(caffiene)后接种，HR的面积有所增加。进一步用胞内Ca2+通道抑制剂(herapin、RR和8-Br-cADPR)预处理，结果herapin对HR的影响呈浓度依赖型，而RR和8-Br-cADPR对HR没有明显作用。据此提出，胞内Ca2+可能参与小麦抵抗叶锈菌侵染过程中钙信号的形成，且这一过程主要通过IP3途径完成。

关键词: 小麦, 叶锈菌, Ca2+, 过敏性反应

Abstract:

With injecting drugs to affect intracellular calcium stores and calcium channel in Wheat (Triticum aestivum) leaves, we investigated hypersensitive reaction (HR) changes in wheat leaves invaded by leaf rust (Puccinia triticina). The results showed that the intracellular calcium chelators (BAPTA-AM) could obviously reduce the area of dying cells caused by HR. The higher the concentration was, the smaller the area of dying cell was. While intracellular calcium activator (caffeine) was injected into wheat leaves, HR could also be detected. The HR area increased with caffeine concentration. Calcium channel blockers (heparin, RR, and 8-Br-cADPR) were separately injected into wheat leaves before inoculation. The results showed that there was a concentration-depended effect on HR with herapin, and no obviously effect with RR and 8-Br-cADPR. Therefore, we deduced that intracellular calcium might involve in the formation of calcium signal transduction through IP3 pathway during infection of wheat leaf rust fungus.

Key words: Wheat(Triticum aestivum L.), Puccinia triticina, Calcium, Hypersensitive response



[1] Xu H, Heath M C. Role of calcium in signal transduction during the hypersensitive response caused by basidiospore-derived infection of the cowpea rust fungus. Plant Cell, 1998, 10: 585–597





[2] Guan C-L(关春蕾), Hou C-Y(侯春燕), Wang D-M(王冬梅). The role of drugs affecting calcium metabolism and calcium channel on HR induced by wheat leaf rust fungus. J Agric Univ Hebei (河北农业大学学报), 2006, 29(6): 4–8 (in Chinese with English abstract)





[3] Bush D S. Calcium regulation in plant cells and its role in signaling. Annu Rev Plant Physiol Plant Miol Biol, 1995, 46: 95–122





[4] Sanders D, Brownlee C, Harper J F. Communicating with calcium. Plant Cell, 1999, 11: 691–706





[5] Alexandre J, Lassalles J P, Kado R T. Opening of Ca2+ channels in isolated red beet root vacuole membrane by inositol 1,4,5-trisphosphate. Nature, 1990, 343: 567–570





[6] Allen G J, Mair S R, Sandens D. Release of Ca2+from individual plant vacuoles by both InsP3 and cyclic ADP-ribose. Science, 1995, 268: 735–737





[7] Ward J M, Schroeder J I. Calcium-activated K+-channels and calcium induced calcium release by slow vacuolar ion channels in guard cell vacuoles implicated in the control of stomatal closure. Plant Cell, 1994, 6: 669–683





[8] Deng E-X(邓恩新), Wang W-Z(王文忠), Wang Z-X(王智炘). A simple equipment for injecting solution to plant leaves. Plant Physiol Commun (植物生理学通讯), 1992, 28(4): 296 (in Chinese)





[9] Rohringer K, Kim W K. An optical brighter for fluorescence microscopy of fungal plant parasites in leaves. Phytopathology, 1977, 67: 808–810





[10] Renelt A, Colling C, Hahlbrock K, Nürnberger T, Parker J E, Sacks W, Scheel D. Studies on elicitor recognition and signal transduction in plant defence. J Exp Bot, 1993, 44 (suppl): 257–268





[11] Mithöfer A, Ebel J, Bhagwat A A, Boller T, Neuhaus-Url G. Transgenic aequorin monitors cytosolic transients in soybean cells challenged with β-glucan or chitin elicitors. Planta, 1999, 207: 566–574





[12] Durner J, Wendehenne D, Klessig D F. Defence gene induction in tobacco by nitric oxide, cyclic GMP, and cyclic ADP-ribose. Proc Natl Acad Sci USA, 1998, 95: 10328–10333





[13] Klessig D F, Durner J, Noad R, Navarre D A, Wendehenne D, Kumar D, Zhou J M, Zhang S, Kachroo P, Trifa Y, Pontier D, Lam E, Silva H. Nitric oxide and salicylic acid signalling in plant defences. Proc Natl Acad Sci USA, 2000, 97: 8849–8855





[14] Lamotte O, Gould K, Lecourieux D, Sequeira-Legrand A, Lebrun-Garcia A, Durner J, Pugin A, Wendehenne D. Analysis of nitric oxide signalling functions in tobacco cells challenged by the elicitor cryptogein. Plant Physiol, 2004, 135: 516–529





[15] Vandelle E, Poinssot B, Wendehenne D, Bentéjac M, Pugin A. Integrated signalling network involving calcium, nitric oxide, and active oxygen species but not mitogen-activated protein kinases in BcPG1-elicited grapevine defences. Mol Plant Microbe Interactions, 2006, 19: 429–440





[16] Lecourieux D, Mazars C, Pauly N, Ranjeva R, Pugin A. Analysis and effects of cytosolic free calcium increases in response to elicitors in Nicotiana plumbaginifolia cells. Plant Cell, 2002, 14: 2627–2641
[1] 翟胜男, 曹新有, 李豪圣, 李吉虎, 李法计, 刘金栋, 夏先春, 吕莹莹, 马瑞峰, 王颖, 耿洪伟, 刘建军. 小麦Pod-A1、Pod-D1和Pod-2D位点等位变异对籽粒过氧化物酶活性的遗传效应分析[J]. 作物学报, 2026, 52(6): 1593-1603.
[2] 习千辉, 徐梓瑗, 刘梦梦, 王宏艺, 郎凯琳, 井震海, 陈锋, 赵磊. 小麦籽粒铜含量的全基因组关联分析及候选基因预测[J]. 作物学报, 2026, 52(6): 1604-1617.
[3] 毛嘉琦, 黄朋雨, 赵佳佳, 郑兴卫, 武棒棒, 郝宇琼, 屈非, 刘成, 马朋涛, 郑军. 山西小麦品种白粉病抗性评价及抗病基因分子检测[J]. 作物学报, 2026, 52(6): 1669-1681.
[4] 胡川, 赵凯男, 黄修利, 吴金芝, 任开明, 王贺正, 付国占, 黄明, 李友军. 一次灌溉下耕作方式和氮肥用量对旱地小麦产量和品质的影响[J]. 作物学报, 2026, 52(6): 1830-1846.
[5] 陈雪燕, 何华川, 李政嘉, 董新盼, 李藕琪, 刘小云, 李丹萍, 陈志伟, 刘国霞, 吕胜源, 吴印莹, 赵振东, 曹新有, 万何平. 水培盐碱复合胁迫下‘济麦60’苗期根系有机酸分泌动态变化及其转录调控机制[J]. 作物学报, 2026, 52(6): 1859-1875.
[6] 高沛阳, 李瑾璇, 董宇奎, 石玉, 张振, 张永丽. 测墒补灌下小麦分蘖发生和成穗对施氮量的响应[J]. 作物学报, 2026, 52(6): 1847-1858.
[7] 张献丰, 郭利建, 李康春, 孔斌雪, 刘玉芳, 车卓, 杨德龙. 小麦ABHD6基因家族鉴定与粒重功能标记开发[J]. 作物学报, 2026, 52(6): 1711-1727.
[8] 王壮壮, 武紫君, 张永新, 张芯源, 袁丽雪, 陈如雪, 刘世举, 段剑钊, 冯伟, 王同朝, 王永华. 豫东南黏壤潮土区水氮优化协同提高冬小麦产量和氮素利用效率[J]. 作物学报, 2026, 52(5): 1501-1521.
[9] 何万龙, 耿洪伟, 张飞飞, 米克热阿依·阿巴白克热, 罗紫洋, 李鹏程, 周钊宇, 程宇坤. 基于深度学习的小麦重要病害图像识别系统的研究[J]. 作物学报, 2026, 52(5): 1401-1417.
[10] 张振, 冯连杰, 石玉, 于振文, 张永丽. 节水补灌下不同穗型小麦产量形成差异研究[J]. 作物学报, 2026, 52(5): 1522-1535.
[11] 侯思宇, 王国璀, 韦金贵, 谢玮欣, 殷文, 樊志龙, 柴强, 胡发龙. 绿肥配施化学氮肥对西北干旱灌区小麦干物质积累及产量形成的影响[J]. 作物学报, 2026, 52(4): 1208-1219.
[12] 尚云秋, 赵竹, 陈欢, 丁永刚, 乔玉强, 李玮, 张向前, 曹承富, 杜世州. 长期定位耕作方式对雨养小麦籽粒灌浆和产量形成的影响[J]. 作物学报, 2026, 52(4): 1236-1250.
[13] 乔宇馨, 李程越, 康晓玉, 张鑫琪, 贾绍辉, 刘倩, 曹亚丽, 史鑫蕊, 郝兴宇, 李萍. 基于APSIM模型的长期免耕秸秆覆盖对旱地小麦增产效应研究[J]. 作物学报, 2026, 52(4): 1181-1192.
[14] 李灿, 张喜伟, 朱博涛, 张沛沛. 小麦GSK激酶TaSK41的功能分析及互作蛋白的筛选[J]. 作物学报, 2026, 52(3): 677-687.
[15] 侯洁, 付朵朵, 武海峰, 郝宇琼, 郑兴卫, 武棒棒, 周凯, 李晓华, 郑军, 赵佳佳. 山西省小麦地方品种的染色体多样性及遗传效应分析[J]. 作物学报, 2026, 52(3): 746-763.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2