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作物学报 ›› 2010, Vol. 36 ›› Issue (09): 1490-1497.doi: 10.3724/SP.J.1006.2010.01490

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

利用分子标记筛查小麦相似品种(系)

王立新1,李宏博1,廖琴2,邱军2,常利芳1,刘丽华1,任立平1,高新欢1,赵昌平1,*   

  1. 1北京市农林科学院北京杂交小麦工程技术研究中心，北京 100097；2全国农业技术推广服务中心，北京 100026
  • 收稿日期:2010-01-08 修回日期:2010-04-17 出版日期:2010-09-12 网络出版日期:2010-06-11
  • 通讯作者: 赵昌平, E-mail: bjhwc2003@yahoo.com.cn, Tel: 010-51503968
  • 基金资助:

    本研究由北京市农业育种基础研究创新平台项目（D08070500690801），国家科技支撑计划项目（2006BAD01A02-4）和国家高技术研究与发展计划（863计划）项目（2009AA101102）资助。

Screening Wheat cultivars with Genetic Similarity Using Molecular Markers

WANG Li-Xin1,LI Hong-Bo1,LIAO Qin2,QIU Jun2,CHANG Li-Fang1,LIU Li-Hua1,REN Li-Ping1,GAO Xin-Huan1,ZHAO Chang-Ping1*   

  1. 1Beijing Engineering and Technique Research Center for Hybrid Wheat, Beijing 100097, China；National Agriculture Technology Spreading Service, Beijing 100026, China
  • Received:2010-01-08 Revised:2010-04-17 Published:2010-09-12 Published online:2010-06-11
  • Contact: ZHAO Chang-Ping, E-mail: bjhwc2003@yahoo.com.cn, Tel: 010-51503968

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被引次数

6

摘要： 在每年的小麦区域试验中均发现一些参试品系高度相像或与审定品种高度相像，为此需建立从大量品种中筛查相似性品种的方法。本文比较了547个国家冬小麦区域试验品系及134个国审品种的分子标记遗传相似系数(GS)和主要农艺性状，发现GS大于0.95的绝大多数品种(系)间主要农艺性状和表型高度相似，分子标记比对结果与田间比较结果吻合程度高达95%。依此确立了用分子标记筛查相似品种的方法体系，解决了无法从大量品种中查找相似品种(系)的问题。

关键词: 小麦品种, 分子标记, 遗传相似系数

Abstract: Cultivars with similar traits are often found in the national winter wheat (Triticum aestivum L.) regional trials. Molecular markers have been introduced to identify such cultivars efficiently and accurately in screening wheat cultivars with genetic similarity. In this study, 681 wheat cultivars and lines were assessed using 105 molecular markers, including 63 genome-SSRs, 21 EST-SSRs, and 21 AFLP-SCARs. These markers were classified into core primer (21 pairs), first-grade standby primer (29 pairs), and second-grade standby primer (55 pairs). The 681 cultivars and lines consisted of 134 released cultivars and 547 lines evaluated in the national winter wheat regional trials from 2005 to 2009. Cultivars were primarily screened with the core primers, and those with similar fingerprint in 19-21 loci were further tested with the first-grade standby primers, and only the confirmed similar cultivars (GS > 0.90) were conduced the final detection by the second-grade standby primers. Finally, the cultivars with total GS value (at least 100 loci) higher than 0.95 were considered as similar ones. These similar cultivars were continuously identified on the basis of main agronomic traits. The results showed that 95% of the similar cultivars detected by molecular markers had identical or highly similar agronomic traits, and only two pairs of cultivars with GS > 0.95 had distinct performances in agronomic traits. Thus, the laboratory identification had the accordance with the field test by 95%, suggesting that these molecular markers are effective in bulk screening similar cultivars in wheat regional trials.

Key words: Winter wheat, molecular marker, genomic similarity coefficient

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