作物学报 ›› 2010, Vol. 36 ›› Issue (10): 1615-1623.doi: 10.3724/SP.J.1006.2010.01615
• 作物遗传育种·种质资源·分子遗传学 • 下一篇
孙慧敏1,张军1,2,赵团结1,*,盖钧镒1,*
SUN Hui-Min1,ZHANG Jun1,2,ZHAO Tuan-Ji1,*,GAI Jun-Yi1,*
摘要: 利用自然群体进行关联分析是检测目标性状QTL、揭示其遗传基础的有效方法。对国内黄淮和南方地区和东亚、东南亚、南亚291份大豆品种幼苗期耐淹性和64个SSR标记的关联分析结果表明,整个群体由国内和国外2个不同的亚群体组成,2个亚群均存在连锁不平衡。在群体1 (国内)中分别检测到相对死苗率、相对失绿率、相对萎蔫率的关联位点3、7和12个,群体2 (国外)中相应位点6、3和5个;多个位点兼与2个或者3个耐淹性状关联;部分关联位点与连锁定位结果一致。在2个群体中分别筛选出3个耐淹性状减效最大(最耐淹)的优异等位变异24个和22个。相对死苗率优异等位变异在黄淮、南方地区5个主要系谱中分布不同,育种轮次间有波动。结合基因型和耐性表现,从国内材料中优选出合豆2号、黔豆3号、诱变31、南农493-1,从国外材料中优选出PI208432、PI377576、PI481690等耐淹载体材料,为耐淹育种奠定材料和标记辅助选择育种的基础。
[1] Kruglyak L. Prospects for whole-genome linkage disequilibrium mapping of common disease genes. Nat Genet, 1999, 22: 139–144 [2] Gupta P K, Rustgi S, Kulwal P L. Linkage disequilibrium and association studies in higher plants: Present status and future prospects. Plant Mol Biol 2005, 57: 461–485 [3] Aranzana M J, Kim S, Zhao K Y, Bakker E, Horton M, Jakob K, Lister C, Molitor J, Shindo C, Tang C L, Toomajian C, Traw B, Zheng H G, Nergelson J, Dean C, Marjoram P, Nordborg M. Genome-wide association mapping in Arabidopsis identifies previously Known flowering time and pathogen resistance genes. PLoS Genet, 2005, 1: 60 [4] Zhu Y L, Song Q J, Hyten D L, Tassell C P van, Matukumalli L K, Grimm D R, Hyatt S M, Fickus E W, Young N D, Cregan P B. Single-nucleotide polymorphisms in soybean. Genetics, 2003, 163: 1123–1134 [5] Wang R-H(王荣焕), Wang T-Y(王天宇), Li Y(黎裕). Application of association analysis in molecular evaluation of crop germplasm resources. J Plant Genet Resour(植物遗传资源学报), 2007, 8(3): 366–372 (in Chinese with English abstract) [6] Jin L(金亮), Bao J-S(包劲松). Progress on the trait-marker association analysis in plants. Mol Plant Breed (分子植物育种), 2009, 7(6): 1048–1063 (in Chinese with English abstract) [7] Hyten D L, Choi I Y, Song Q J, Shoemaker R C, Nelson R L, Costa J M, Specht J E, Cregan P B. Highly variable patterns of linkage disequilibrium in multiple soybean populations. Genetics, 2007, 175: 1937–1944 [8] Wen Z X(文自翔), Zhao T-J(赵团结), Zheng Y-Z(郑永战), Liu S-H(刘顺湖), Wang C-E(王春娥), Wang F(王芳), Gai J-Y(盖钧镒). Association analysis of agronomic and quality traits with SSR markers in Glycine max and Glycine soja in China: I. Population structure and associated markers. Acta Agron Sin (作物学报), 2008, 34(7): 1169–1178 (in Chinese with English abstract) [9] Jun T H, Van K J, Kim M Y, Lee S H, Walker D R. Association analysis using SSR markers to find QTL for seed protein content in soybean. Euphytica, 2008, 162:179–191 [10] Zhang J(张军), Zhao T-J(赵团结), Gai J-Y(盖钧镒). Genetic diversity, specificity and population differentiation of soybean cultivars in Asia.Sci Agric Sin (中国农业科学), 2008, 41(11): 3511–3520 (in Chinese with English abstract) [11] Abe J, Xu D H, Suzuki Y, Kanazawa A, Shimamoto Y. Soybean germplasm pools in Asia revealed by nuclear SSRs. Theor Appl Genet, 2003, 106: 445–453 [12] Zhang J(张军), Zhao T-J(赵团结), Gai J-Y(盖钧镒). Association analysis of agronomic trait QTLs with SSR markers in released soybean cultivars. Acta Agron Sin(作物学报), 2008, 34(12): 2059–2069 (in Chinese with English abstract) [13] Wang F(王芳), Zhao T-J(赵团结), Gai J-Y(盖钧镒). Evaluation, eco-region characterization and elite germplasm identification of submergence tolerance at seeding stage in wild and cultivated soybeans. Soybean Sci (大豆科学), 2007, 26(6): 828–834 (in Chinese with English abstract) [14] Sun H-M(孙慧敏), Zhao T-J(赵团结), Gai J-Y(盖钧镒). Evaluation and identification of elite germplasm with submergence tolerance at young seedling stage of soybean cultivars released in Asia. Soybean Sci (大豆科学), 2010, 29(1): 1–6 (in Chinese with English abstract) [15] Pritchard J K, Stephens M, Donnelly P. Inference of population structure using multilocus genotype data. Genetics, 2000, 155: 945–959 [16] Edward Buckler Lab. Maize Diversity Research. [2007-01-30] [http://www.maizegenetics.net/bioinformatics/tassel/] [2007-09-20] [17] Githiri S M, Watanabe S, Harada K, Takahashi R. QTL analysis of flooding tolerance in soybean at an early vegetative growth stage. Plant Breed, 2006, 125: 613–618 [18] Cornelious B, Chen P, Chen Y, Leon N D, Shannon J G, Wang D. Identification of QTLs underlying water-logging tolerance in soybean. Mol Breed, 2005, 16: 103–112 [19] Wang J, McClean P E, Lee R, Goos R J, Helms T. Association mapping of iron deficiency chlorosis loci in soybean (Glycine max L. Merr.) advanced breeding lines. Theor Appl Genet, 2008, 116: 777–787 [20] Reyna N, Cornelious B, Shamnon J G, Sneller C H. Evaluation of a QTL for waterlogging tolerance in southern soybean germplasm. Crop Sci, 2003, 43: 2077–2082 [21] Wang F(王芳), Yu D-Y(喻德跃), Chen S-Y(陈受宜), Gai J-Y(盖钧镒). Inheritance and QTL analysis of submergence tolerance at seedling stage in soybean [Glycine max (L.) Merr.]. Acta Agron Sin (作物学报), 2008, 34(5): 748–753 (in Chinese with English abstract) [22] Zhang J(张军), Zhao T-J(赵团结), Gai J-Y(盖钧镒). Inheritance of elite alleles of yield and quality traits in pedigrees of major cultivar families released in Huang-Huai Valleys and southern China. Acta Agron Sin (作物学报), 2009, 35(2): 191–202 (in Chinese with English abstract) |
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