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作物学报 ›› 2011, Vol. 37 ›› Issue (04): 629-634.doi: 10.3724/SP.J.1006.2011.00629

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

水稻颖壳和浆片异常突变体ahl的基因定位

王增**,李云峰**,马娇,任德勇,王德仲,游小庆,桑贤春,何光华*   

  1. 西南大学水稻研究所 / 转基因植物与安全控制重庆市重点实验室，重庆400716
  • 收稿日期:2010-09-17 修回日期:2011-01-06 出版日期:2011-04-12 网络出版日期:2011-02-24
  • 通讯作者: 何光华, E-mail: hegh@swu.edu.cn
  • 基金资助:

    本研究由国家自然科学基金项目(31071390)，重庆市杰出青年基金项目(2008BA1033)和重庆市良种创新工程项目(CSTC,2010AA1013)资助。

Gene Mapping of a Novel Mutant ahl in Rice

WANG Zeng**,LI Yun-Feng**,MA Jiao,REN De-Yong,WANG De-Zhong,YOU Xiao-Qing,SANG Xian-Chun,HE Guang-Hua*   

  1. Rice Research Institute, Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops, Southwest University, Chongqing 400716, China
  • Received:2010-09-17 Revised:2011-01-06 Published:2011-04-12 Published online:2011-02-24
  • Contact: 何光华, E-mail: hegh@swu.edu.cn

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2354

被引次数

9

摘要： 研究水稻花发育基因对于水稻相关性状的分子育种具有十分重要的意义。本研究报道一个水稻颖壳和浆片异常突变体ahl (abnormal hull and lodicule)，来源于优良恢复系缙恢10号的EMS诱变群体。该突变体内外稃变小并发生严重扭曲，浆片顶端伸长。内外稃异常导致灌浆后米粒变小、畸形，千粒重下降。该性状遗传稳定，受一对隐性基因控制，利用群体分离分析法(bulked segregation analysis，BSA)将AHL基因定位在第2染色体上的SSR标记RM14153与RM14167之间，遗传距离分别为1.19 cM和1.34 cM，物理距离为226 kb。研究结果为AHL基因的图位克隆和功能研究奠定了基础。

关键词: 水稻, 颖壳和浆片异常突变体, 遗传分析, 基因定位

Abstract: Theresearch on rice flower development gene is very important in molecular breeding of rice for related traits. A rice flower mutant abnormal hull and lodicule (ahl) was identified from Jinhui 10 (Oryza sativa L. ssp. indica) treated by EMS. In ahl mutant, lemma and palea minished and significantly twisted along with the apparently elongated apical lodicules. This minished lemma and palea led toabnormal kernelsafter grain filling, meanwhile, the 1000-grain weight decreased. The trait wassteadily inherited, and controlled by a pair of recessive gene.Using bulked segregation analysis method AHL was restricted between SSR markers RM14153 and RM14167, with genetic distances of 1.34 cM and 1.19 cM respectively, which covered an approximate 226 kb region on the chromosome 2. The result is useful for further map-based cloning and functional analysis of AHL gene.

Key words: Rice (Oryza sativa L.), abnormal hull and lodicule, Genetic analysis, Gene mapping

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