欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2011, Vol. 37 ›› Issue (05): 811-819.doi: 10.3724/SP.J.1006.2011.00811

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

利用SRAP标记分析我国甜菜三大产区骨干材料的遗传多样性

王茂芊1,2,3,吴则东1,2,3,王华忠1,2,3,*   

  1. 1黑龙江省普通高校甜菜遗传育种重点实验室 / 黑龙江大学，黑龙江哈尔滨 150080；2中国农业科学院北方糖料作物资源与利用重点开放实验室，黑龙江哈尔滨 150080；3中国农业科学院甜菜研究所 / 黑龙江大学农作物研究院，黑龙江哈尔滨 150080
  • 收稿日期:2010-10-08 修回日期:2011-01-06 出版日期:2011-05-12 网络出版日期:2011-03-24
  • 通讯作者: 王华忠, E-mail: wwhhzz0451@sohu.com, Tel: 0451-86609494
  • 基金资助:

    本研究由国家“十一五”科技支撑计划重点项目(2006BAD01A06)资助。

Analysis of Genetic Diversity of Major Sugar Beet Varieties from Three Regions of China with SRAP Markers

WANG Mao-Qian1,2,3, WU Ze-Dong1,2,3, WANG Hua-Zhong1,2,3,*   

  1. 1 The Key Laboratory of Sugar Beet Genetic Breeding, Colleges of Heilongjiang Province / Heilongjiang University, Harbin 150080, Heilongjiang; 2 The Key Laboratory of North Sugar Crop Resource and Utilization, Chinese Academy of Agricultural Sciences, Harbin 150080, Heilongjiang; 3 Sugar Beet Research Institute of Chinese Academy of Agricultural Sciences / Crop Academy of Heilongjiang University, Harbin 150080, Heilongjiang
  • Received:2010-10-08 Revised:2011-01-06 Published:2011-05-12 Published online:2011-03-24
  • Contact: 王华忠, E-mail: wwhhzz0451@sohu.com, Tel: 0451-86609494

PDF

1504

被引次数

23

摘要： 利用SRAP分子标记, 选用甜菜中SRAP的88对引物组合分别对4个经济性状差异较大的代表性品系(高产型、高产低糖抗丛根病型、标准型、中产高糖抗褐斑病型)进行扩增，筛选出有效引物组合33对。采用筛选的33对引物检测全国三大产区的241份甜菜材料及9份外国材料，扩增到719条带，其中多态性条带459条，多态性条带的比率平均为63.8%。利用MEGA3.1软件中的Compute over-all mean计算，组内品种间平均遗传距离为0.4165，平均遗传相似系数为0.6593。遗传相似系数平均值为外国品种(0.7528)>单胚品系(0.6945)>多胚四倍体品系(0.6816)>多胚二倍体品系(0.6612)。利用MEGA3.1软件，在遗传距离0.20处，将供试材料分为4大类群。结果表明，我国三大产区供试材料遗传多样性丰富，其中东北产区优于华北与西北产区。利用POPGEN32软件将供试材料与外国品种分为两类，表明我国材料与外国品种的遗传基础存在较大差异。

关键词: 甜菜, SRAP标记, 种质资源, 遗传多样性

Abstract: Shortage ofsugar beet germlasm resources results in the lag in researches of breeding and molecular biology. It is necessary to analyze the major varieties from three major production regions of China. Eighty-eight primer pairs were used to amplify the genomic DNA from leaves of four types of sugar beet varieties with different economic traits, which contain high yield type; high yield, low sugar and Rhizomania resistant type; standard type; medium yield, high sugar and anti-brown spot type, and 33 of which were obtained to be with availability. Two hundred and forty-one varieties from three major regions of China and nine varieties from abroad were detected with 33 primer pairs of SRAP markers. A total of 719 unambiguous bands were obtained, 459 of which were polymorphic. The average ratio of polymorphic bands was 63.8%. Compute over-all mean showed that genetic distance was 0.4165, genetic similarity among varieties was 0.6593, the genetic similarities were 0.7528 among foreign varieties, 0.6945 among monogerm varieties, 0.6816 among polygerm tetraploid varieties, and 0.6612 among polygerm diploid varieties. A total of 250 varieties were divided into four cluster groups based on cluster analysis by MEGA3.1 (at intercept of 0.2). Each genepool from three major region showed the highest. The varieties from China and abroad were classified into two different groups by POPGEN32. This indicated definite difference in the genetic background between foreign and native varieties.s of sugar beet production in China showed high level of genetic diversity, of which the Northeast genepool

Key words: Sugar beet, SRAP marker, Germplasm resource, Genetic diversity

[1]Lu Y-C(路运才), Wang H-Z(王华忠). Genetic analysis of sugarbeet polyploid varieties by RAPD markers. Sugar Crops China (中国糖料), 2006, (3): 5−8 (in Chinese with English abstract)
[2]Yu X(于歆), Xu D-C(徐德昌), Cui J(崔杰), Wu Y-Y(吴永英), Xia H-M(夏红梅). AFLP relationship and genealogy analysis of sugar beet sumfull series varieties (lines). Mol Plant Breed (分子植物育种), 2004, 2(2): 229−234 (in Chinese with English abstract)
[3]Tian Z-H(田自华), Zhang Z-Y(张子义), Zhang J-F(张剑峰), Shi S-D(史树德), Bai W(白薇), Shao J-W(邵金旺). RAPD analysis of mitochondrial DNA of cytoplasmic male sterile line and its maintainer line in sugarbeet. Mol Plant Breed (分子植物育种), 2004, 2(6): 817−828 (in Chinese with English abstract)
[4]Tian Z-H(田自华), Shi S-D(史树德), Zhang J-F(张剑峰), Zhang Z-Y(张子义), Shao J-W(邵金旺). RAPD analysis of chloroplast DNA of cytoplasmic male sterile line and its maintainer line in sugarbeet. Acta Agric Boreali-Sin (华北农学报), 2004, 19(1): 54−56 (in Chinese with English abstract)
[5]Wang H-Z(王华忠), Wu Z-D(吴则东), Wang X-W(王晓武), Fang Z-Y(方智远). Analysis of the genetic diversity in different types of sugar beets by SRAP and SSR Markers. Acta Agron Sin (作物学报), 2008, 34(1): 37−46 (in Chinese with English abstract)
[6]Wang H-Z(王华忠), Wu Z-D(吴则东), Han Y(韩英), Wang C-L(王丛玲). Optimization of sugarbeet SRAP-PCR reaction system. Sugar Crops China (中国糖料), 2007, (2): 1−4 (in Chinese with English abstract)
[7]Tautz D. Hypervariability of simple sequences as a general source for polymorphic DNA markers. Nucl Acids Res, 1989, 17: 6463−6471
[8] Liu L-W(柳李旺), Gong Y-Q(龚义勤), Huang H(黄浩), Zhu X-W(朱献文). A new molecular marker-SRAP and TRAP and its application. Hereditas (遗传), 2004, 26(5): 777−781 (in Chinese with English abstract)
[9]Budak H, Shearman R C, Parmaksiz I, Gaussoin R E, Riordan T P, Dweikat I. Molecular characterization of buffalograss germplasm using sequence-related amplified polymorphism markers. Theor Appl Genet, 2004, 108: 328−334
[10]Lin Z-X(林忠旭), Zhang X-L(张献龙), Nie Y-C(聂以春), He D-H(贺道华), Wu M-Q(吴茂清). Construction of SRAP linkage map in cotton. Chin Sci Bull (科学通报), 2003, 48(15): 1676−1679 (in Chinese with English abstract)
[11]Lin Z-X(林忠旭), Zhang X-L(张献龙), Nie Y-C(聂以春). Evaluation of application of a new molecular marker SRAP on analysis of F2 segregation population and genetic diversity in cotton. Acta Genet Sin (遗传学报), 2004, 31(6): 622−626 (in Chinese with English abstract)
[12]Li Y(李严), Zhang C-Q(张春庆). Studies on genetic diversity with a molecular marker SRAP of watermelon hybrids. Acta Hort Sin (园艺学报), 2005, 32(4): 643−647 (in Chinese with English abstract)
[13]Wen Y-C(文雁成), Wang H-Z(王汉中), Shen J-X(沈金雄), Liu G-H(刘贵华), Zhang S-F(张书芬). Analysis of genetic diversity and genetic basis of Chinese rapeseed cultivars (Brassica napus L.) by sequence-related amplified polymorphism markers. Sci Agric Sin (中国农业科学), 2006, 39(2): 246−256 (in Chinese with English abstract)
[14]Liu Y-G(刘月光), Teng Y-Y(滕永勇), Pan C(潘辰), Han Y-C(韩延闯), Zhou M-Q(周明全), Hu Z-L(胡中立). Cluster analysis of Nelumbo based on SRAP markers. Amino Acids Biotic Res (氨基酸和生物资源), 2006, 28(1): 29−32 (in Chinese with English abstract)
[15]Wu J(吴洁), Tan W-F(谭文芳), He J-R(何俊蓉), Pu Z-G(蒲志刚), Wang D-Y(王大一), Zhang Z-S (张正圣), Zhan F-F(詹付凤), Yan W-Z(阎文昭). Construct on of SRAP linkage map and QTL mapping for starch content in sweet potato. Mol Plant Breed (分子植物育种), 2005, 3(6): 841−845 (in Chinese with English abstract)
[16]Budak H, Shearman R C, Parmaksiz I, Gaussoin R E, Riordan T P, Dweikat I. Molecular characterization of buffalograss germplasmusing sequence-related amplified polymorphism markers. Theor Appl Genet, 2004, 108: 328−334
[17]Chen K-S(陈昆松), Li F(李方), Xu C-J(徐昌杰), Zhang S-L(张上隆), Fu C-X(傅承新). An efficient macro- method of genomic DNA isolation from Actinidia chinensis leaves. Hereditas (遗传), 2004, 26(4): 529−531 (in Chinese with English abstract)
[18]Li G, Quiros C F. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor Appl Genet, 2001, 103: 455−461
[1] 肖颖妮, 于永涛, 谢利华, 祁喜涛, 李春艳, 文天祥, 李高科, 胡建广. 基于SNP标记揭示中国鲜食玉米品种的遗传多样性[J]. 作物学报, 2022, 48(6): 1301-1311.
[2] 张钰坤, 陆赢, 崔看, 夏石头, 刘忠松. 芥菜种子颜色调控基因TT8的等位变异及其地理分布分析[J]. 作物学报, 2022, 48(6): 1325-1332.
[3] 陈静, 任佰朝, 赵斌, 刘鹏, 张吉旺. 叶面喷施甜菜碱对不同播期夏玉米产量形成及抗氧化能力的调控[J]. 作物学报, 2022, 48(6): 1502-1515.
[4] 陈小红, 林元香, 王倩, 丁敏, 王海岗, 陈凌, 高志军, 王瑞云, 乔治军. 基于高基元SSR构建黍稷种质资源的分子身份证[J]. 作物学报, 2022, 48(4): 908-919.
[5] 胡亮亮, 王素华, 王丽侠, 程须珍, 陈红霖. 绿豆种质资源苗期耐盐性鉴定及耐盐种质筛选[J]. 作物学报, 2022, 48(2): 367-379.
[6] 张加康, 李斐, 史树德, 杨海波. 内蒙古地区甜菜临界氮浓度稀释模型的构建及应用[J]. 作物学报, 2022, 48(2): 488-496.
[7] 张鹤, 蒋春姬, 殷冬梅, 董佳乐, 任婧瑶, 赵新华, 钟超, 王晓光, 于海秋. 花生耐冷综合评价体系构建及耐冷种质筛选[J]. 作物学报, 2021, 47(9): 1753-1767.
[8] 王琰琰, 王俊, 刘国祥, 钟秋, 张华述, 骆铮珍, 陈志华, 戴培刚, 佟英, 李媛, 蒋勋, 张兴伟, 杨爱国. 基于SSR标记的雪茄烟种质资源指纹图谱库的构建及遗传多样性分析[J]. 作物学报, 2021, 47(7): 1259-1274.
[9] 刘少荣, 杨扬, 田红丽, 易红梅, 王璐, 康定明, 范亚明, 任洁, 江彬, 葛建镕, 成广雷, 王凤格. 基于农艺及品质性状与SSR标记的青贮玉米品种遗传多样性分析[J]. 作物学报, 2021, 47(12): 2362-2370.
[10] 孙倩, 邹枚伶, 张辰笈, 江思容, Eder Jorge de Oliveira, 张圣奎, 夏志强, 王文泉, 李有志. 基于SNP和InDel标记的巴西木薯遗传多样性与群体遗传结构分析[J]. 作物学报, 2021, 47(1): 42-49.
[11] 孙志广, 王宝祥, 周振玲, 方磊, 迟铭, 李景芳, 刘金波, Bello Babatunde Kazeem, 徐大勇. 水稻萌发耐淹性种质资源筛选及QTL定位[J]. 作物学报, 2021, 47(1): 61-70.
[12] 赵孟良,王丽慧,任延靖,孙雪梅,侯志强,杨世鹏,李莉,钟启文. 257份菊芋种质资源表型性状的遗传多样性[J]. 作物学报, 2020, 46(5): 712-724.
[13] 张红岩,杨涛,刘荣,晋芳,张力科,于海天,胡锦国,杨峰,王栋,何玉华,宗绪晓. 利用EST-SSR标记评价羽扇豆属(Lupinus L.)遗传多样性[J]. 作物学报, 2020, 46(3): 330-340.
[14] 刘易科,朱展望,陈泠,邹娟,佟汉文,朱光,何伟杰,张宇庆,高春保. 基于SNP标记揭示我国小麦品种(系)的遗传多样性[J]. 作物学报, 2020, 46(02): 307-314.
[15] 张晓军,肖进,王海燕,乔麟轶,李欣,郭慧娟,常利芳,张树伟,阎晓涛,畅志坚,武宗信. 小偃麦衍生品系的赤霉病抗性评价[J]. 作物学报, 2020, 46(01): 62-73.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2