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作物学报 ›› 2011, Vol. 37 ›› Issue (11): 2059-2065.doi: 10.3724/SP.J.1006.2011.02059

• 耕作栽培·生理生化 • 上一篇    下一篇

T6VS?6AL染色体易位与小麦籽粒HMW-GS和GMP积累的关系

徐甜甜1，蔡剑1，汪波1，亓增军2，戴廷波1，曹卫星1，姜东1,*   

  1. 1南京农业大学 / 农业部南方作物生理生态重点开放实验室 / 江苏省信息农业高技术研究重点实验室, 江苏南京210095; 2作物遗传与种质创新国家重点实验室, 江苏南京210095
  • 收稿日期:2011-02-25 修回日期:2011-06-25 出版日期:2011-11-12 网络出版日期:2011-09-06
  • 通讯作者: 姜东, E-mail: jiangd@njau.edu.cn
  • 基金资助:

    本研究由国家自然科学基金项目(30971734, 31028017, 31000686), 高等学校博士学科点专项科研基金(20090097110009), 国家公益性行业科研专项(200903003), 中央高校基本科研业务费专项资金(KYZ200915), 现代农业产业技术体系(nycytx-03)和江苏省粮食丰产科技工程项目(BE2009426)资助。

T6VS?6AL Chromosome Translocation Does Not Alter Accumulations of High Molecular Weight Glutenin Subunits and Glutenin Macropolymer in Wheat Grain

XU Tian-Tian1,CAI Jian1,WANG Bo,QI Zeng-Jun2,DAI Ting-Bo1,CAO Wei-Xing1,JIANG Dong1,*   

  1. 1 Key Laboratory of Crop Physiology and Ecology in Southern China, the Ministry of Agriculture / Hi-Tech Key Laboratory of Information Agriculture, Jiangsu Province / Nanjing Agricultural University, Nanjing 210095, China; 2 State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2011-02-25 Revised:2011-06-25 Published:2011-11-12 Published online:2011-09-06
  • Contact: 姜东, E-mail: jiangd@njau.edu.cn

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摘要： 从一套由92R137 (普通小麦-簇毛麦T6VS·6AL染色体易位系)和辉县红(地方小麦品种)杂交及以单粒传方法构建的F8重组近交家系(RIL)群体中, 筛选出4个HMW-GS亚基组合相同而蛋白质含量差异较大的代表性家系(含和不含染色体易位片段的家系各一组), 采用SDS-PAGE电泳和切胶比色的亚基定量方法, 研究了不同家系小麦籽粒灌浆期间HMW-GS和GMP含量动态。结果表明, 小麦籽粒各亚基在花后13 d均已形成, 但不同亚基起始形成时间不同；各亚基含量随灌浆进程呈上升趋势, 花后23 d到成熟期为快速积累期。染色体易位片段对籽粒HMW-GS和GMP含量与积累量无显著作用, 而籽粒HMW-GS含量以蛋白含量高的家系高于对应的低蛋白家系。

关键词: 小麦, 重组近交家系, T6VS•6AL, 高分子量谷蛋白亚基(HMW-GS), 谷蛋白大聚合体(GMP)

Abstract:  Four lines from an F8 recombinant inbred line (RIL) population were identified and classed into the the T6VS·6AL and non the T6VS·6AL groups. The grain protein content differed between the groups. The time-course changes in content and accumulation of HMW-GS and GMP during grain filling were detected. All HMW-glutenin subunits were detected at 13 d after anthesis (DAA) in grains of the four lines. However, the initial formation time differed within subunits. The content and accumulation of the HMW-GS increased during the whole grain filling period, and the rapid accumulation occurred from 23 DAA to maturity. The content and accumulation of HMW-GS and GMP showed no significant differences between the two groups. However, content and accumulation of HMW-GS of the high grain protein content lines were higher than those of the contrasting low protein content lines. In addition, grain gliadin content was found higher in the lines containing the T6VS·6AL chromosome translocation segment than in the contrasting lines.

Key words: Triticum aestivum L., Recombinant inbred lines (RILs), T6VS•6AL, High molecular weight glutenin subunits (HMW-GS), Glutenin macropolymer (GMP)

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