棉属野生种克劳茨基棉第7染色体上马克隆值QTL的挖掘与定位

doi:10.3724/SP.J.1006.2012.00447

摘要/Abstract

摘要： 为了深入挖掘和利用棉属野生种克劳茨基棉(Gossypium klotzschianum)的优异等位基因，构建了一个(陆地棉泗棉2号×克劳茨基棉)×泗棉2号的BC₁F₂群体，对纤维品质性状初步定位，单标记相关分析表明位于第7染色体上的SSR标记NAU1362与马克隆值表现极显著相关。进一步选择在第7染色体上含有克劳茨基棉渐渗片段的BC₁F₂单株与轮回亲本泗棉2号回交，构建BC₂F₃和BC₂F₄分离群体，通过两年的田间重复试验验证该QTL的位置与效应。结果表明，该QTL (qFMIC-7-1)在BC₂F₃、BC₂F₄世代均被检测到，位于相同的标记区间，分别可以解释9.0%、8.8%的表型变异，增效基因来源于野生种克劳茨基棉，与BC₁F₂群体定位结果基本一致。同时在第7染色体上检测到另一马克隆值QTL (qFMIC-7-2)，同样在BC₂F₃、BC₂F₄两个世代均能够被检测到，分别可以解释3.7%、4.7%的表型变异，但增效基因均来源于泗棉2号。

关键词: 棉花, 克劳茨基棉, 马克隆值, QTL定位

Abstract: G. klotzschianum, carrying the elite alleles, is a diploid species of D genome originated from Galapagos Island, harboring the lethal genes to cause inconsistency of apical bud growth. In this research, we overcame the obstacle and established a BC₁F₂ population derived from (Simian2× G. klotzschianum) ×Simian2 (G. hirsutum). The SSR marker NAU1362 on chromosome 7 showed significant correlation with micronaire value by single marker analysis. The BC₂F₃ and BC₂F₄ populations were developed from the cross between BC₁F₂individuals containing target segments of chromosome 7 from G. klotzschianum and recurrent parent Simian2. The software Cartographer (V2.5) and the composite interval mapping were further employed to identify quantitative trait loci (QTL) associated with fiber micronaire in two generations. The fiber micronaire QTL qFMIC-7-1 identified in BC₁F₂ population was confirmed in BC₂F₃ and BC₂F₄, which explained 9.0% and 8.8% of the phenotypic variance, respectively. The G. klotzschianum allele decreased the fiber micronaire value. Another micronaire QTL qFMIC-7-2 on chromosome 7 was also detected in BC₂F₃and BC₂F₄ generations with phenotypic variance of 3.7% and 4.7%, respectively. Simian2 was genotyped as decreased micronaire value. This study provides valuable resources for effectively utilization of potential elite genes from G. klotzschianum.

Key words: Cotton, G. klotzschianum, Micronaire, QTL mapping

徐鹏, 朱静, 张香桂, 倪万潮, 徐英俊, 沈新莲. 棉属野生种克劳茨基棉第7染色体上马克隆值QTL的挖掘与定位[J]. 作物学报, 2012, 38(03): 447-453.

XU Peng, ZHU Jing, ZHANG Xiang-Gui, NI WAN-Chao, XU Yang-Jun, SHEN Xin-Lian. Molecular Mapping and Identification of QTLs for Fiber Micronaire on Chromosome 7 from Gossypium klotzschianum[J]. Acta Agron Sin, 2012, 38(03): 447-453.

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链接本文: https://zwxb.chinacrops.org/CN/10.3724/SP.J.1006.2012.00447

https://zwxb.chinacrops.org/CN/Y2012/V38/I03/447

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