欢迎访问作物学报，今天是 2025年5月10日 星期六
作物学报

作物学报 ›› 2012, Vol. 38 ›› Issue (11): 2108-2114.doi: 10.3724/SP.J.1006.2012.02108

• 耕作栽培·生理生化 • 上一篇    下一篇

甘蓝型油菜DH群体苗期抗旱性的评价

李真2，梅淑芳2，梅忠2，刘芳1，周广生1*, 吴江生1   

  1. 1 华中农业大学植物科学技术学院，湖北武汉 430070；2 金华职业技术学院农业与生物工程学院，浙江金华 321000
  • 收稿日期:2012-03-18 修回日期:2012-07-05 出版日期:2012-11-12 网络出版日期:2012-09-10
  • 通讯作者: 周广生, E-mail: zhougs@mail.hzau.edu.cn, Tel: 027-87281507
  • 基金资助:

    本研究由国家公益性行业(农业)科研专项(201203096), 国家科技支撑项目(2010BAD01B09)和国家现代农业产业技术体系建设项目(CARS-13)。

Evaluation of Drought Resistance in Rapeseed (Brassica napus L.) DH Lines at Seedling Stage

LI Zhen2,MEI Shu-Fang2,MEI Zhong2,LIU Fang1,ZHOU Guang-Sheng1,*,WU Jiang-Sheng1   

  1. 1 College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; 2 Department of Agriculture and Bioengineering, Jinhua College of Profession and Technology, Jinhua 321000, China
  • Received:2012-03-18 Revised:2012-07-05 Published:2012-11-12 Published online:2012-09-10
  • Contact: 周广生, E-mail: zhougs@mail.hzau.edu.cn, Tel: 027-87281507

PDF

1740

被引次数

26

摘要：

中国油菜主产区常常受到秋冬旱和春旱危害而影响产量及品质。本文通过盆栽试验，以株高(PH)、根长(RL)、地上部干重(SDW)、根干重(RDW)、根冠比(R/S)、总干重(TDW) 6个性状的抗旱系数作为抗旱性评价指标，对甘蓝型油菜DH(doubled haploid)群体的118个株系及其亲本进行苗期抗旱性评价，筛选极端抗旱DH系。结果表明，与对照相比，干旱严重抑制了甘蓝型油菜苗期的生长，6个评价指标均表现出显著差异，其中根干重的变异系数最大；在对照和干旱条件下，群体各株系各性状均表现出超亲连续分离，大部分呈正态分布，抗旱条件下的分离更为明显；相关性分析表明，地上部干重、根干重和总干重抗旱系数可作为甘蓝型油菜苗期抗旱性的主要评价指标；

关键词: 甘蓝型油菜, 苗期, 抗旱性, 评价指标

Abstract:

Drought stress in autumn, winter and spring has endangered the production and quality of rapeseed in China.The objective of the study was to evaluate drought resistance of the rapeseed (Brassica napus L.) DH (doubled haploid) population and screen the most drought-resistant DH lines. For phenotyping, the rapeseed lines from DH population [(Huyou 15×5900) F4 × (No2127-17×275B) F4] were grown in plastic pots. Six traits including plant height, root length, shoot dry weight (SDW), root dry weight (RDW), root/shoot ratio and total dry weight (TDW) together with DRC (drought resistance coefficient) of all the traits were used to evaluate the drought resistance of the 118 DH lines and their parents. The results showed that the six responsive traits changed significantly under the drought conditions compared with the control in the experiment and the effects of drought were much more severe on RDW than on other traits. All traits showed transgressive and continuous distribution under both drought and control conditions. The correlation among all the traits investigated in the study indicated that DRC of SDW, RDW and TDW could be used as available evaluation indices for drought resistance. Lines 032, 034, and 035 screened from the population were the most drought-resistant lines and 006, 091, and 104 were the most drought-sensitive lines.

Key words: Brassica napus, Seedling stage, Drought resistance, Evaluation index

[1]Yang C-J(杨春杰), Cheng Y(程勇), Zou C-S(邹崇顺), Zhang X-K(张学昆), Zheng P-Y(郑普英), Li G-Y(李桂英), Yang C(杨畅). Combining ability and genetic effects of germination ability in different Brassica napus L. cultivar under simulated drought stress. Acta Agron Sin (作物学报), 2008, 34(10): 1744–1749 (in Chinese with English abstract)



[2]Akihiko K, Chandra B R, Manikanda B N, Shu F K. Phenotypic and genotypic analysis of drought-resistance traits for development of rice cultivars adapted to rainfed environments. Field Crops Res, 2008, 109: 1–23



[3]Varshney R K, Langridge P, Graner A. Application of genomics to molecular breeding of wheat and barley. Adv Genet, 2007, 58: 21–55



[4]Yadav R S, Sehgal D, Vadez V. Using genetic mapping and genomics approaches in understanding and improving drought tolerance in pearl millet. J Exp Bot, 2011, 62: 397–408



[5]Araus J L, Slafer G A, Reynolds M P, Royo C. Plant breeding and drought in C3 cereals: what should we breed for? Ann Bot, 2002, 89: 925–940



[6]Ren H B, Gao Z H, Chen L, Wei K F, Liu J, Fan Y J, Davies W J, Jia W S, Zhang J H. Dynamic analysis of ABA accumulation in relation to the rate of ABA catabolism in maize tissues under water deficit. J Exp Bot, 2007, 58: 211–219



[7]Zhang Z-M(张智猛), Dai L-X(戴良香), Ding H(丁红), Chen D-X(陈殿绪), Yang W-Q(杨伟强), Song W-W(宋文武), Wan S-B(万书波). Identification and evaluation of drought resistance in different peanut varieties widely grown in northern China. Acta Agron Sin (作物学报), 2012, 38(3): 495–504 (in Chinese with English abstract)



[8]Liu J-L(刘吉利), Zhao C-X(赵长星), Wu N(吴娜), Wang Y-F(王月福), Wang M-L(王铭伦). Effects of drought and rewatering at seedling stage on photosynthetic characteristics and water use efficiency of peanut. Sci Agric Sin (中国农业科学), 2011, 44(3): 469–476 (in Chinese with English abstract)



[9]Wang H-Z(王贺正), Ma J(马均), Li X-Y(李旭毅), Li Y(李艳), Zhang R-P(张荣萍), Wang R-Q(汪仁全). Relationship between some physiological and biochemical characteristics and drought tolerance at rice flowering stage. Sci Agric Sin (中国农业科学), 2007, 40(2): 399–404 (in Chinese with English abstract)



[10]Wu B(武斌), Li X-H(李新海), Xiao M-J(肖木辑), Xie C-X(谢传晓), Hao Z-F(郝转芳), Li M-S(李明顺), Zhang S-H(张世煌). Genetic variation in fifty-three maize inbred lines in relation to drought tolerance at seedling stage. Sci Agric Sin (中国农业科学), 2007, 40(4): 665–676 (in Chinese with English abstract)



[11]Yang S-P(杨守萍), Chen J-M(陈加敏), Liu Y(刘莹), Yu D-Y(喻德跃), Gai J-Y(盖钧镒). Identification and analysis of drought tolerance and root traits of seedling in soybean. Soybean Sci (大豆科学), 2005, 24(3): 176–182 (in Chinese with English abstract)



[12]Xu R(徐蕊), Wang Q-B(王启柏), Zhang C-Q(张春庆), Wu C-L(吴承来). Drought-resistance evaluation system of maize inbred. Sci Agric Sin (中国农业科学), 2009, 42(1): 72–84 (in Chinese with English abstract)



[13]Qi X-S(祁旭升), Wang X-R(王兴荣), Xu J(许军), Zhang J-P(张建平), Mi J(米君). Drought-resistance evaluation of flax germplasm at adult plant stage. Sci Agric Sin (中国农业科学), 2010, 43(15): 3076–3087 (in Chinese with English abstract)



[14]Guo X-S(郭雪松). Evaluation on Drought Tolerance Ability of Rapeseed Resource. MS Thesis of Southwest University, 2009



[15]Zhu Z-H(朱宗河), Zheng W-Y(郑文寅), Zhang X-Y(张学昆). Principal component analysis and comprehensive evaluation on morphological and agronomic traits of drought tolerance in rapeseed (Brassica napus L.). Sci Agric Sin (中国农业科学), 2011, 44(9): 1775–1787 (in Chinese with English abstract)



[16]Wang H-Z(王贺正), Li Y(李艳), Ma J(马均), Zhang R-P(张荣萍), Li X-Y(李旭毅), Wang R-Q(汪仁全). Screening Indices of drought resistance during seedling stage in rice. Acta Agron Sin (作物学报), 2007, 33(9): 1523–1529 (in Chinese with English abstract)



[17]Luo M-L(罗明亮). Combining Ability and Genetic Effect Analysis of Drought Tolerant Traits during Seedling Stage in Brassica napus L. MS Thesis of Southwest University, 2010 (in Chinese with English abstract)



[18]Gong M(龚明). Screening methods and indexes of drought resistencein crops and comprehensive evaluation. J Yunnan Agric Univ (云南农业大学学报), 1989, (1): 73–81 (in Chinese with English abstract)



[19]Lafitte H R, Guan Y S, Yan S, Li Z K. Whole plant responses, key processes, and adaptation to drought stress: the case of rice. J Exp Bot, 2007, 58: 169–175



[20]Ashraf M. Inducing drought tolerance in plants: recent advances. Biotechnol Adv, 2010, 28: 169–183



[21]Roberto T, Silvio S. Genomics-based approaches to improve drought tolerance of crops. Trends Plant Sci, 2006, 11: 405–412
[1] 陈松余, 丁一娟, 孙峻溟, 黄登文, 杨楠, 代雨涵, 万华方, 钱伟. 甘蓝型油菜BnCNGC基因家族鉴定及其在核盘菌侵染和PEG处理下的表达特性分析[J]. 作物学报, 2022, 48(6): 1357-1371.
[2] 秦璐, 韩配配, 常海滨, 顾炽明, 黄威, 李银水, 廖祥生, 谢立华, 廖星. 甘蓝型油菜耐低氮种质筛选及绿肥应用潜力评价[J]. 作物学报, 2022, 48(6): 1488-1501.
[3] 王兴荣, 李玥, 张彦军, 李永生, 汪军成, 徐银萍, 祁旭升. 青稞种质资源成株期抗旱性鉴定及抗旱指标筛选[J]. 作物学报, 2022, 48(5): 1279-1287.
[4] 袁大双, 邓琬玉, 王珍, 彭茜, 张晓莉, 姚梦楠, 缪文杰, 朱冬鸣, 李加纳, 梁颖. 甘蓝型油菜BnMAPK2基因的克隆及功能分析[J]. 作物学报, 2022, 48(4): 840-850.
[5] 黄成, 梁晓梅, 戴成, 文静, 易斌, 涂金星, 沈金雄, 傅廷栋, 马朝芝. 甘蓝型油菜BnAPs基因家族成员全基因组鉴定及分析[J]. 作物学报, 2022, 48(3): 597-607.
[6] 王瑞, 陈雪, 郭青青, 周蓉, 陈蕾, 李加纳. 甘蓝型油菜白花基因InDel连锁标记开发[J]. 作物学报, 2022, 48(3): 759-769.
[7] 胡亮亮, 王素华, 王丽侠, 程须珍, 陈红霖. 绿豆种质资源苗期耐盐性鉴定及耐盐种质筛选[J]. 作物学报, 2022, 48(2): 367-379.
[8] 张海燕, 解备涛, 姜常松, 冯向阳, 张巧, 董顺旭, 汪宝卿, 张立明, 秦桢, 段文学. 不同抗旱性甘薯品种叶片生理性状差异及抗旱指标筛选[J]. 作物学报, 2022, 48(2): 518-528.
[9] 赵雪, 周顺利. 玉米抗茎倒伏能力相关性状与评价研究进展[J]. 作物学报, 2022, 48(1): 15-26.
[10] 高芳, 刘兆新, 赵继浩, 汪颖, 潘小怡, 赖华江, 李向东, 杨东清. 北方主栽花生品种的源库特征及其分类[J]. 作物学报, 2021, 47(9): 1712-1723.
[11] 王艳花, 刘景森, 李加纳. 整合GWAS和WGCNA筛选鉴定甘蓝型油菜生物产量候选基因[J]. 作物学报, 2021, 47(8): 1491-1510.
[12] 李杰华, 端群, 史明涛, 吴潞梅, 柳寒, 林拥军, 吴高兵, 范楚川, 周永明. 新型抗广谱性除草剂草甘膦转基因油菜的创制及其鉴定[J]. 作物学报, 2021, 47(5): 789-798.
[13] 唐鑫, 李圆圆, 陆俊杏, 张涛. 甘蓝型油菜温敏细胞核雄性不育系160S花药败育的形态学特征和细胞学研究[J]. 作物学报, 2021, 47(5): 983-990.
[14] 周新桐, 郭青青, 陈雪, 李加纳, 王瑞. GBS高密度遗传连锁图谱定位甘蓝型油菜粉色花性状[J]. 作物学报, 2021, 47(4): 587-598.
[15] 李书宇, 黄杨, 熊洁, 丁戈, 陈伦林, 宋来强. 甘蓝型油菜早熟性状QTL定位及候选基因筛选[J]. 作物学报, 2021, 47(4): 626-637.
Viewed
Full text
790
HTML PDF
Just accepted Online first Issue Just accepted Online first Issue
0 0 0 0 0 790

  From Others local
  Times 14 776
  Rate 2% 98%

Abstract
416
Just accepted Online first Issue
0 0 416
  From Others local
  Times 89 327
  Rate 21% 79%

Cited
Web of Science  Crossref   ScienceDirect  Search for Citations in Google Scholar >>
 
This page requires you have already subscribed to WoS.
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2