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作物学报 ›› 2012, Vol. 38 ›› Issue (11): 2122-2130.doi: 10.3724/SP.J.1006.2012.02131

• 研究简报 • 上一篇    下一篇

小麦叶绿素缺失突变体Mt135的叶绿体基因差异表达分析

夏家平,郭会君,谢永盾,赵林姝,古佳玉,赵世荣,李军辉,刘录祥*   

  1. 中国农业科学院作物科学研究所 / 农作物基因资源与基因改良国家重大科学工程 / 国家农作物航天诱变技术改良中心, 北京100081
  • 收稿日期:2012-04-19 修回日期:2012-06-20 出版日期:2012-11-12 网络出版日期:2012-09-10
  • 通讯作者: 刘录祥, E-mail: luxiang@263.net.cn; Tel: 010-62122719
  • 基金资助:

    本研究由国家航天育种工程(发改高技[2003]138号), 国家高技术研究发展计划(863计划)项目(2012AA100402), 国家公益性行业(农业)科研专项(201103007)和国际原子能机构项目(CRP14195和RAS5056)资助。

Differential Expression of Chloroplast Genes in Chlorophyll-Deficient Wheat Mutant Mt135 Derived from Space Mutagenesis

XIA Jia-Ping,GUO Hui-Jun,XIE Yong-Dun,ZHAO Lin-Shu,GU Jia-Yu,ZHAO Shi-Rong,LI Jun-Hui,LIU Lu-Xiang*   

  1. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement / National Center of Space Mutagenesis for Crop Improvement, Beijing 100081, China
  • Received:2012-04-19 Revised:2012-06-20 Published:2012-11-12 Published online:2012-09-10
  • Contact: 刘录祥, E-mail: luxiang@263.net.cn; Tel: 010-62122719

摘要:

小麦叶绿素缺失突变体Mt135自交后代稳定表现绿株、条纹株和白化株3种类型, 其中条纹株白色组织和白化株的叶绿体数目和结构发生突变, 完全失去光合能力。为研究该突变体叶绿体基因表达与光合作用的关系, 采用实时荧光定量PCR技术, 分析了白化株和条纹株的叶绿体基因表达。在白化株中共检测到40个差异表达基因, 涉及4类功能(编码光反应相关蛋白、编码叶绿体内能量代谢相关酶、核糖体合成和tRNA合成), 包括18个上调表达和22个下调表达基因;在条纹株中共检测到13个上调表达基因, 其表达变化趋势与在白化株中一致。白化株的差异表达基因中, 编码光系统II、I结构蛋白的psbpsaycf等基因家族的基因表达量显著下调;多个编码核糖体蛋白大、小亚基的基因表达量改变, 尤其是核糖体蛋白小亚基编码基因rps14和23S rRNA的编码基因23S rDNA表达量显著下调。推测Mt135突变性状与参与光反应相关蛋白的编码基因、叶绿体内能量代谢相关酶的编码基因、核糖体合成相关基因以及tRNA合成相关基因表达量的改变密切相关。

关键词: 小麦, 叶绿素缺失突变体, 叶绿体基因, 表达差异

Abstract:

The leaf color ofchlorophyll-deficient mutant Mt135 (Triticum aestivum L.) shows albino, striped, and green phenotypes. The number and structure of chloroplasts in the albino plant and albino tissue of striped plants are significantly different from those in the wild type, which results in the complete loss of photosynthetic function in albino plant/tissue. The expression alteration of chloroplast genes in young seedlings of albino Mt135 and wild type was studied using RT-PCR technique, and 40 genes were identified to be differentially expressed in the albino plant/tissue, including 18 genes up-regulated and 22 genes down-regulated. These genes categorized into four groups encoded proteins responding photoreaction (31%), enzymes of energy metabolism in chloroplast (27%), ribosome biogenesis (25%), and tRNA biosynthesis (17%). The alteration trends of 13 up-regulated genes detected in the striped plants were similar to those in albino seedlings. Among these differentially expressed genes, gene families of psb, psa, and ycf encoding structural proteins of photosystem II and I were down-regulated significantly, genes encoding ribosomal protein subunits were altered, especially expressions of gene rps14 encoding small ribosomel subunit protein and 23S rDNA encoding 23S rRNA were down-regulated significantly. It was concluded that the differential expression of these genes related with proteins responding photoreaction, enzymes of energy metabolism in chloroplast, ribosome biogenesis and tRNA biosynthesis may induce the phenotype of mutant Mt135.

Key words: Triticum aestivum L., Chlorophyll-deficient mutant, Chloroplast gene, Differential expression

[1]Zhang L-K(张力科), Li Z-B(李志彬), Liu H-Y(刘海燕), Li R-H(李如海), Chen M-Y(陈满元), Chen A-G(陈爱国), Qian Y-L(钱益亮), Hua Z-T(华泽田), Gao Y-M(高用明), Zhu L-H(朱苓华), Li Z-K(黎志康). Study on morphological structure and genetic mapping of two novel leaf color mutants in rice. Sci Agric Sin (中国农业科学), 2010, 43(2): 223-229 (in Chinese with English abstract)



[2]Zhang H, Zhang D, Han S, Zhang X, Yu D. Identification and gene mapping of a soybean chlorophyll-deficient mutant. Plant Breed, 2011, 130: 133-138



[3]Cheng H-L(程红亮), Chen J-F(陈甲法), Ding J-Q(丁俊强), Wu Y-J(吴建宇). Genetic analysis and gene mapping of a leaf mutant in maize. Acta Agric Boreali-Sin (华北农学报), 2011, 26(3): 7-10 (in Chinese with English abstract)



[4]Jiang Y(江媛), He Y(何筠), Fan S-L(范术丽), Yu J-N(俞嘉宁), Song M-Z(宋美珍).The identification and analysis of RNA editing sites of 10 chlooroplast protein-coding genes from virescent mutant of Gossypium hirstum. Cotton Sci (棉花学报), 2011, 23(1): 3-9 (in Chinese with English abstract)



[5]Sun J-Y(孙捷音), Zhang N-H(张年辉), Du L-F(杜林方). Chlorophyll biosynthesis in a chlorophyll b-deficient oilseed rape mutant Cr3529. Acta Bot Boreail-Occident Sin (西北植物学报), 2007, 27(10): 1962-1966 (in Chinese with English abstract)



[6]Lin H-H(林宏辉), Du L-F(杜林方), Jia Y-J(贾勇炯), Liang H-G(梁厚果), Tang Z-H(汤泽生). Isolation and comparison of thylakoid membarenes pigment-proteins from wile type an dmutant barley. Acta Bot Boreal-Occident Sin (西北植物学报), 1997, 17(1): 34-38 (in Chinese with English abstract)



[7]Huang X-Q(黄晓群), Zhao X-H(赵海新), Dong C-L(董春林), Sun Y-Y(孙业盈), Wang P-R(王平荣), Deng X-J(邓晓建). Chlorophyll-deficit rice mutants and their research advances in biology. Acta Bot Boreali-Occid Sin (西北植物学报), 2005, 25(8): 1685-1691 (in Chinese with English abstract)



[8]Zhong H-B(赵洪兵), Guo H-J(郭会君), Zhao L-S(赵林姝), Gu J-Y(古佳玉), Zhao S-R(赵世荣), Li J-H(李军辉), Liu L-X(刘录祥). A temperature-sensitive winter wheat chlorophyll mutant derived from space mutagenesis. J Nucl Agric Sci (核农学报), 2010, 24(6): 1110-1116 (in Chinese with English abstract)



[9]Hou D Y, Xu H, Du G Y, Lin J T, Duan M, Guo A G. Proteome analysis of chloroplast proteins in stage albinism line of winter wheat (Triticum aestivum L.) FA85. BMB Rep, 2009, 42: 450-455



[10]Wang B-L(王保莉), Guo A-G(郭蔼光), Wang P-H(汪沛洪). Changes of chlorophyll metabolism during the albino stage of a winter mutant. Acta Bot Sin (植物学报), 1996, 38(7): 557-562 (in Chinese with English abstract)



[11]Yang L(杨莉), Guo A-G(郭蔼光), Guan X(关旭). Studies on the chloroplast ultrastructure of “stage albinism line the winter wheat” (SA) mutant during the albescent period. Acta Agric Boreali-Occid Sin (西北农业学报), 2003, 12(4): 64-67 (in Chinese with English abstract)



[12]Su X-J(苏小静), Wang P-H(汪沛洪), Wang Y-J(王永吉), Li P-G(李丕皋), Feng R-M(封如敏). Studies on the mechanism of albino characteristic of winter wheat mutant “the albinism line”: Ⅳ. Primary studies on the changes of transpiration, photosynthesis and respiration at albino stage. Acta Univ Agric Boreali-Occident (西北农业大学学报), 1996, 24(1): 41-44 (in Chinese with English abstract)



[13]Iwai M, Suzuki T, Dohmae N, Inoue Y, Ikeuchi M. Absence of the PsbZ subunit prevents association of PsbK and Ycf12 with the PSII complex in the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1. Plant Cell Physiol, 2007, 48: 1758-1763



[14]Kashino Y, Takahashi Takeshi, Inoue-Kashino N, Ban A, Ikeda Y, Satoh K, Sugiura Miwa. Ycf12 is a core subunit in the photosystem II complex. Biochim Biophys Acta, 2007, 1767: 1269-1275



[15]Toshiharu S, Katsumi S, Katsumi U, Yoshiki N, Tsuneyoshi K, Takashi H. The chloroplast clpP gene, encoding a proteolytic subunit of ATP-dependent protease, is indispensable for chloroplast development in tobacco. Plant Cell Physiol, 2001, 42: 264-273



[16]Rogalski M, Schottler M A, Thiele W, Schulze W X, Bock R. Rpl33, a nonessential plastid-encoded ribosomal protein in tobacco, is required under cold stress conditions. Plant Cell, 2008, 20: 2221-2237



[17]Siniauskaya M, Naydenov N, Davydenko O, Nakamura C. Macroarray for studying chloroplast gene expression profiles associated with the initial development of wheat. In: Rudi A, Russell E, Evans L, Peter L, Michael M L, eds. Proceedings of the 11th International Wheat Genetics Symposium. Sydney University Press, 2008. pp 1-3



[18]Matsuoka Y, Yamazaki Y, Ogihara Y, Tsunewaki K. Whole chloroplast genome comparison of rice, maize, and wheat: implications for chloroplast gene diversification and phylogeny of cereals. Mol Biol Evol, 2002, 19: 2084-2091



[19]Ogihara Y, Isono K, Kojima T, Endo A, Hanaoka M, Shiina T, Terachi T, Utsugi S, Murata M, Mori N. Structural features of a wheat plastome as revealed by complete sequencing of chloroplast DNA. Mol Gen Genomics, 2002, 266: 740-746



[20]Zhao H B, Guo H J, Zhao L S, Gu J Y, Zhao S R, Li J H, Liu L X. Agronomic traits and photosynthetic characteristics of chlorophyll-deficient wheat mutant induced by spaceflight environment. Acta Agron Sin, 2011, 37: 119-126



[21]Zhao H-B(赵洪兵). Studies on chlorophyll-deficent wheat mutant induced by spaceflight environment. MS Thesis of Chinese Academy of Agricultural Sciences, 2010. pp 41-42 (in chinese with English abstract)



[22]Vandesompele J, Katleen D P, Filip P, Bruce P, Nadine V R, Anne D P, Frank S. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol, 2002, 3: research 0034.1-0034.11, DOI: 10.1186/gb-2002-3-7-research0034



[23]Gutierrez R A, Stokes T L, Thum K, Xu X, Obertello M, Katari M S, Tanurdzic M, Dean A, Nero D C, Mcclung C R, Coruzzi G M. Systems approach identifies an organic nitrogen-responsive gene network that is regulated by the master clock control gene CCA1. Proc Natl Acad Sci USA, 2008, 105: 4939-4944



[24]Brunner S, Hurni S, Herren G, Kalinina O, von Burg S, Zeller S L, Schmid B, Winzeler M, Keller B. Transgenic Pm3b wheat lines show resistance to powdery mildew in the field. Plant Biotech J, 2011, 9: 897-910



[25]Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods, 2001, 25: 402-408



[26]Allen J F, Wilson B M, Puthiyaveetil S, Nield J. A structural phylogenetic map for chloroplast photosynthesis. Trends Plant Sci, 2011, 16: 645-655



[27]Pakrasi H B. Genetic analysis of the form and function of photosystem I and photosystem II. Annu Rev Genet, 1995, 29: 755-776



[28]Boudreau E, Takahashi Y, Lemieux C, Turmel M, Rochaix J D. The chloroplast ycf3 and ycf4 open reading frames of Chlamydomonas reinhardtii are required for the accumulation of the photosystem I complex. EMBO J, 1997, 16: 6095-6104



[29]Wang Y X, Suo B, Zhao T F, Qu X F, Yuan L G, Zhao X J. Zhao H J. Effect of nitric oxide treatment on antioxidant responses and psbA gene expression in two wheat cultivars during grain filling stage under drought stress and rewatering. Acta Physiol Plant, 2011, 33: 1923-1932



[30]Iwai M, Suzuki T, Kamiyama A, Sakurai I, Dohmae N, Inoue Y, Ikeuchi M. The PsbK subunit is required for the stable assembly and stability of other small subunits in the PSII complex in the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1. Plant Cell Physiol, 2010, 51: 554-560



[31]Umate P, Schwenkert S, Karbat I, Dal Bosco C, Mlcòchová L, Volz S, Zer H, Herrmann R G, Ohad I, Meurer J. Deletion of PsbM in tobacco alters the QB site properties and the electron flow within photosystem II. J Biol Chem, 2007, 282: 9758-9676



[32]Iwai M, Katoh H, Katayama M, Ikeuchi M. PSII-Tc protein plays an important role in dimerization of photosystem II. Plant Cell Physiol, 2004, 45: 1809-1816



[33]Monde R A, Greene J C, Stern D B. Disruption of the petB-petD intergenic region in tobacco chloroplasts affects petD RNA accumulation and translation. Mol Gen Genet, 2000, 263: 610-618



[34]Mäenpää P, Gonzalez E B, Chen L, Khan M S, Gray J C, Aro E M. The ycf 9 (orf 62) gene in the plant chloroplast genome encodes a hydrophobic protein of stromal thylakoid membranes. J Exp Bot, 2000, 51(GMP Special Issue): 375-382



[35]Swiatek M, Kuras R, Sokolenko A, Higgs D, Olived J, Cinquee G, Müllera B, Eichackera L A, Sternc D B, Bassie R, Herrmanna R G, Wollman F A. The chloroplast gene ycf9 encodes a photosystem II (PSII) core subunit, PsbZ, that participates in PSII supramolecular architecture. Plant Cell, 2001, 13: 1347-1368



[36]Ashby M K, Houmard J, Mullineaux C W. The ycf27 genes from cyanobacteria and eukaryotic algae: distribution and implications for chloroplast evolution. FEMS Microbiol Lett, 2002, 214: 25-30



[37]Peter E, Wallner T, Wilde A, Grimm B. Comparative functional analysis of two hypothetical chloroplast open reading frames (ycf) involved in chlorophyll biosynthesis from Synechocystis sp. PCC6803 and plants. J Plant Physiol, 2011, 168: 1380-1386



[38]Yamori W, Takahashi S, Makino A, Price G D, Badger M R, Susanne V C. The roles of ATP synthase and the cytochrome b6/f complexes in limiting chloroplast electron transport and determining photosynthetic capacity. Plant Physiol, 2010, 155: 956-962



[39]Shen H, Walters D E, Mueller D M. Introduction of the chloroplast redox regulatory region in the yeast ATP synthase impairs cytochrome c oxidase. J Biol Chem, 2008, 283: 32937-32943



[40]Kuroda H, Maliga P. The plastid clpP1 protease gene is essential for plant development. Nature, 2003, 425: 86-89



[41]Rogalski M, Ruf S, Bock R. Tobacco plastid ribosomal protein S18 is essential for cell survival. Nucl Acids Res, 2006, 34: 4537-4545



[42]Fleischmann T T, Scharff L B, Alkatib S, Hasdorf S, Schottler M A, Bock R. Nonessential plastid-encoded ribosomal proteins in tobacco: a developmental role for plastid translation and implications for reductive genome evolution. Plant Cell, 2011, 23: 3137-3155



[43]Santis-Maciossek G D, Kofer W, Bock A, Schoch S, Maier R M, Wanner G, Diger W R, Koop H U, Herrmann R G. Targeted disruption of the plastid RNA polymerase genes rpoA, B and C1: molecular biology biochemistry and ultrastructure. Plant J, 1999, 18: 477-489



[44]Allison L A, Simon L D, Maligal P. Deletion of rpoB reveals a second distinct transcription system in plastids of higher plants. EMBO J, 1996, 15: 2802-2809



[45]Rumeau D, Linka N B, Beyly A, Louwagie M, Garin J, Peltier G. New subunits NDH-M, -N, and -O, encoded by nuclear genes, are essential for plastid ndh complex functioning in higher plants. Plant Cell, 2005, 17: 219-232



[46]Wang L Y, Ou-Yang M, Li Q N, Zou M J, Guo J K, Ma J F, Lu C M, Zhang L X, The Arabidopsis chloroplast ribosome recycling factor is essential for embryogenesis and chloroplast biogenesis. Plant Mol Biol, 2010, 74: 47-59



[47]Chi W, Mao J, Li Q N, Ji D L, Zou M J, Lu C M, Zhang L X. Interaction of the pentatricopeptide-repeat protein DELAYED GREENING 1 with sigma factor SIG6 in the regulation of chloroplast gene expression in Arabidopsis cotyledons. Plant J, 2010, 64: 14-25



[48]Karaca M, Saha S, Franklin E C, Jenkins J N, Read J J, Percy R G. Molecular and cytological characterization of a cytoplasmic-specific mutant in pima cotton. Euphytica, 2004, 139: 187-197

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