氨基酸合成抑制剂类除草剂诱导油菜雄性不育效果评价

doi:10.3724/SP.J.1006.2014.00264

摘要/Abstract

摘要：

甘蓝型油菜对以乙酰乳酸合酶为靶标的磺酰脲类等除草剂很敏感, 其中部分除草剂具有很强的化学杀雄作用。本文通过油菜抽薹期叶面喷施试验, 比较26种能够抑制氨基酸生物合成的除草剂及复配剂对甘蓝型油菜雄蕊育性的影响。结果显示除过双草醚、麦喜、胺苯磺隆外, 其余23种除草剂均对油菜具有不同程度杀雄作用。其中600 mg hm^–2的咪唑乙烟酸、150mg hm^–2的吡嘧磺隆、240 mg hm^–2的烟嘧磺隆、200 mg hm^–2的单嘧磺隆和120 mg hm^–2的氯磺隆具有较高杀雄率, 但容易产生药害。而60~90 mg hm^–2的酰嘧磺隆、苯磺隆及其复配剂对油菜杀雄率大于95%, 持续时间长, 对雌蕊结实性能影响较小, 且在20个甘蓝型油菜品种(系)上杀雄效果稳定, 可以作为油菜等植物的最佳化学杀雄剂活性成分。本试验证明可以从氨基酸合成抑制型除草剂中筛选化学杂交剂, 为进一步开发化学杀雄剂提供了参考。

关键词: 油菜, 雄性不育, 化学杂交剂, 除草剂, 磺酰脲

Abstract:

Oilseed rape is very sensitive to most sulfonylurea herbicides. The active constituent of sulfonylurea herbicides is acetolactate synthase (ALS), which catalyzes the common reaction to synthesize three branched-chain amino acids including leucine, valine, and isoleucine in plants. Some sulfonylurea herbicides have strong gametocidal effect on rapeseed. In the present paper, we evaluated the gametocidal effect of 26 herbicides inhibiting the biosynthesis of amino acids after leaf-spraying them at bolting stage of rapeseed (Brassica napus). Application of 6000 mg ha^–1 glufosinate ammonium and 22 500 mg ha^–1 glyphosate, inhibiting the biosynthesis of glutamine and phenylalanine respectively, caused partially male sterile. Except Bispyribac-sodium, Florasulam + flumetsulam, and Ethametsulfuron, the twenty-three ALS-inhibiting herbicides, including eighteen sulfonylureas, two imidazolinones, one sulfonylamino-carbonyltriazolinone, one triazolopyrimidines, and one pyrimidinylthio (or oxy)-benzoate, could induce male sterility in Brassica napus. Among them, Imazethapyr (600 mg ha^–1), Pyrazosulfuron-ethyl (150 mg ha^–1), Nicosulfuron (240 mg ha^–1), Monosulfuron (200 mg ha^–1), and Chlorsulfuron (120 mg ha^–1) had high gametocidal efficiency which was not stable when the dosage changed slightly, indicating that they would be potential CHAs for Brassica napus by fine adjustment of dosage. Foliar application of Tribenuron-methyl or Amidosulfuron at the doses from 60 to 90 mg ha^–1 on 20 Brassica napus varieties (lines) showed 95% male sterility as well as lower phytotoxicity on pistil fertility. In conclusion, Tribenuron-methyl and Amidosulfuron are the best gametocides for Brassica napus among those herbicides. Our findings of gametocidal effect of those chemicals extend the function of ALS-inhibiting herbicides.

Key words: Brassica napus, Male sterility, Chemical hybridizing agents, Herbicide, Sulfonylurea

于澄宇,何蓓如. 氨基酸合成抑制剂类除草剂诱导油菜雄性不育效果评价[J]. 作物学报, 2014, 40(02): 264-272.

YU Cheng-Yu,HE Bei-Ru. Evaluation of Male-Sterility Induction Effect of Various Amino Acid Biosynthesis Inhibiting-Herbicides on Rapeseed (Brassica napus)[J]. Acta Agron Sin, 2014, 40(02): 264-272.

参考文献

[1]Wall D A, Derksen D A, Friesen L. Canola (Brassica napus) response to simulated sprayer contamination with thifensulfuron and thifensulfuron: tribenuron (2:1). Weed Technol, 1995, 9: 468–476

[2]Vancetovic J, Vidakovic M, Stefanovic L, Simic M. Imazethapyr resistance found in the material of the MRI, Zemun Polje, gene bank. Maydica, 2007, 52: 235–238

[3]李殿荣, 李永红, 任军荣, 田建华, 杨建利, 李建厂. 油菜高油种质+化学诱导雄性不育杂优利用模式及其应用技术.西北农业学报, 2012, 21(11): 69–74

Li D R, Li Y H, Ren J R, Tian J H, Yang J L, Li J C. A model for utilility of heterosis of high oil rapeseed germplasms based on male sterility induced by chemical hybridization agents and its application technologies. Acta Agric Boreali-Occident Sin, 2012, 21(11): 69–74 (in Chinese with English abstract)

[4]官春云, 王国槐, 李栒, 陈社员, 田森林. 油菜化学杀雄药物、机理和杂种研究. 作物研究, 1990, 4(3): 13–19

Guan C Y, Wang G H, Li S, Chen S Y, Tian S L. Studies on chemical male-gametocide, mechanism and hybrids of rapeseed. Crop Res, 1990, 4(3): 13–19 (in Chinese with English abstract)

[5]官春云, 李栒, 王国槐, 陈社元, 袁晏松. 化学杂交剂诱导油菜雄性不育机理的研究: I. 杀雄剂1号对甘蓝型油菜花药毡绒层和花粉粒形成的影响. 作物学报, 1997, 23: 513–521

Guan C Y, Li X, Wang G H, Chen S Y, Yuan Y S. Studies of mechanism on male sterility in rape induced by chemical hybridization agent: I. The effect on formation of tapetum and pollen induced by male gametocide No.1 in rapeseed (Brassica napus). Acta Agron Sin, 1997, 23: 513–521 (in Chinese with English abstract)

[6]官春云, 王国槐, 赵均田, 李涵庄. 杀雄剂1号诱导油菜雄性不育机理研究. 遗传, 1981, 3(5): 15–17

Guan C Y, Wang G H, Zhao J T, Li H Z. Effect and machanism of male sterility induced by chemical inducing agent 1. Hereditas, 1981, 3(5): 15–17 (in Chinese)

[7]Singh V, Chauhan S V S. Evaluation of three chemical hybridizing agents in Brassica juncea L. Brassica, 2004, 6: 71–73

[8]Singh V, Chauhan S V S. Bud pollination and hybrid seed production in detergent-induced male sterile plants of Brassica juncea. Plant Breed, 2003, 122(5): 421–425

[9]刘绚霞, 董军刚, 刘创社, 董振生, 严自斌, 高晓岚, 高崇玉. 新型化学杀雄剂EN对甘蓝型油菜的杀雄效果及其应用研究. 西北农林科技大学学报, 2007, 35(4): 81–85

Liu X X, Dong J G, Liu C S, Dong Z S, Yan Z B, Gao X L, Gao C Y. Effect and utility of a novel chemical hybridizing agent EN on Brassica napus. J Northwest A&F Univ, 2007, 35(4): 81–85 (in Chinese with English abstract)

[10]张耀文, 尚毅, 李永红, 李建厂, 李殿荣. 新型化学杂交剂SX-1对甘蓝型油菜CMS的作用效果研究. 西北农业学报, 2003, 12(3): 57–61

Zhang Y W, Shang Y, Li Y H, Li J C, Li D R. Study on the effect of a new chemical hybridizing agents SX-1 to the CMS in Brassica napus L. Acta Agric Boreali-Occident Sin, 2003, 12(3): 57–61 (in Chinese with English abstract)

[11]于澄宇, 胡胜武, 张春宏, 俞延军, 何蓓如. 化学杂交剂EXP对油菜的杀雄效果. 作物学报, 2005, 31: 1455–1459

Yu C Y, Hu S W, Zhang C H, Yu Y J, He P R. The effect of chemical hybridizing agent EXP on oilseed rape. Acta Agron Sin, 2005, 31: 1455–1459 (in Chinese with English abstract)

[12]Yu C, Hu S, He P, Zhang C, Yu Y. Inducing male sterility in Brassica napus L. by a sulphonylurea herbicide, tribenuron-methyl. Plant Breed, 2006, 125: 61–64

[13]Yu C Y, Dong J G, Hu S W, He P. Efficiency of a novel gametocide amidosulfuron on rapeseed (Brassica napus). Plant Breed, 2009, 128: 538–540

[14]赵汉红, 付云龙. 化学杀雄剂对三系杂交油菜制种母本微粉控制效果试验. 种子科技, 2010, 28(8): 29–30

Zhao H H, Fu Y L. Control Effect test of chemical hybridizing agent on femal parent tiny powder in seed production of three-line hybrid rape. Seed Sci ＆ Technol, 2010, 28(8): 29–30 (in Chinese with English abstract)

[15]于澄宇, 何蓓如. 植物乙酰乳酸合成酶抑制剂作用方式及机理研究进展. 农药学学报, 2011, 13: 221–227

Yu C Y, He P R. Research progress on the mode and mechanism of action of plant acetolactate synthase inhibitors. Chin J Pesticide Sci, 2011, 13: 221–227 (in Chinese with English abstract)

[16]Duggleby R G, Mccourt J A, Guddat L W. Structure and mechanism of inhibition of plant acetohydroxyacid synthase. Plant Physiol Biochem, 2008, 46: 309–324

[17]Tang Q Y, Zhang C X. Data Processing System (DPS) software with experimental design, statistical analysis and data mining developed for use in entomological research. Insect Sci, 2012, DOI: 10.1111/j.1744-7917.2012.01519.x

[18]Wang G X, Tan M K, Rakshit S, Saitoh H, Terauchi R, Imaizumi T, Ohsako T, Tominaga T. Discovery of single-nucleotide mutations in acetolactate synthase genes by EcoTILLING. Pesticide Biochem Physiol, 2007, 88: 143–148

[19]Andrews C J, Cummins I, Skipsey M, Grundya N, Jepsonb I, Townson J, Edwardsa R. Purification and characterization of a family of glutathione transferases with roles in herbicide detoxification in soybean (Glycine max L.); selective enhancement by herbicides and herbicide safeners. Pesticide Biochem Physiol, 2005, 82: 205–219

[20]Deng F, Hatzios K. Characterization of cytochrome P450-mediated bensulfuron-methyl O-demethylation in rice. Pesticide Biochem Physiol, 2003, 74: 102–115

[21]于澄宇, 董军刚, 何蓓如, 胡胜武. 一种化学杀雄杂交植物去杂保纯的方法. 中国发明专利, ZL200910023162.6, 2009-07-02

Yu C Y, Dong J G, He P R, Hu S W. Method to remove the impurity in hybrid plants produced based on gemetocide. China Invention Patent, ZL200910023162.6, 2009-07-02 (in Chinese)

相关文章 15

[1]	陈松余, 丁一娟, 孙峻溟, 黄登文, 杨楠, 代雨涵, 万华方, 钱伟. 甘蓝型油菜BnCNGC基因家族鉴定及其在核盘菌侵染和PEG处理下的表达特性分析[J]. 作物学报, 2022, 48(6): 1357-1371.
[2]	秦璐, 韩配配, 常海滨, 顾炽明, 黄威, 李银水, 廖祥生, 谢立华, 廖星. 甘蓝型油菜耐低氮种质筛选及绿肥应用潜力评价[J]. 作物学报, 2022, 48(6): 1488-1501.
[3]	黄伟, 高国应, 吴金锋, 刘丽莉, 张大为, 周定港, 成洪涛, 张凯旋, 周美亮, 李莓, 严明理. 芥菜型油菜BjA09.TT8和BjB08.TT8基因调节类黄酮的合成[J]. 作物学报, 2022, 48(5): 1169-1180.
[4]	雷新慧, 万晨茜, 陶金才, 冷佳俊, 吴怡欣, 王家乐, 王鹏科, 杨清华, 冯佰利, 高金锋. 褪黑素与2,4-表油菜素内酯浸种对盐胁迫下荞麦发芽与幼苗生长的促进效应[J]. 作物学报, 2022, 48(5): 1210-1221.
[5]	刘嘉欣, 兰玉, 徐倩玉, 李红叶, 周新宇, 赵璇, 甘毅, 刘宏波, 郑月萍, 詹仪花, 张刚, 郑志富. 耐三唑并嘧啶类除草剂花生种质创制与鉴定[J]. 作物学报, 2022, 48(4): 1027-1034.
[6]	石育钦, 孙梦丹, 陈帆, 成洪涛, 胡学志, 付丽, 胡琼, 梅德圣, 李超. 通过CRISPR/Cas9技术突变BnMLO6基因提高甘蓝型油菜的抗病性[J]. 作物学报, 2022, 48(4): 801-811.
[7]	袁大双, 邓琬玉, 王珍, 彭茜, 张晓莉, 姚梦楠, 缪文杰, 朱冬鸣, 李加纳, 梁颖. 甘蓝型油菜BnMAPK2基因的克隆及功能分析[J]. 作物学报, 2022, 48(4): 840-850.
[8]	黄成, 梁晓梅, 戴成, 文静, 易斌, 涂金星, 沈金雄, 傅廷栋, 马朝芝. 甘蓝型油菜BnAPs基因家族成员全基因组鉴定及分析[J]. 作物学报, 2022, 48(3): 597-607.
[9]	王瑞, 陈雪, 郭青青, 周蓉, 陈蕾, 李加纳. 甘蓝型油菜白花基因InDel连锁标记开发[J]. 作物学报, 2022, 48(3): 759-769.
[10]	赵改会, 李书宇, 詹杰鹏, 李晏斌, 师家勤, 王新发, 王汉中. 甘蓝型油菜角果数突变体基因的定位及候选基因分析[J]. 作物学报, 2022, 48(1): 27-39.
[11]	娄洪祥, 姬建利, 蒯婕, 汪波, 徐亮, 李真, 刘芳, 黄威, 刘暑艳, 尹羽丰, 王晶, 周广生. 种植密度对油菜正反交组合产量与倒伏相关性状的影响[J]. 作物学报, 2021, 47(9): 1724-1740.
[12]	张建, 谢田晋, 尉晓楠, 王宗铠, 刘崇涛, 周广生, 汪波. 无人机多角度成像方式的饲料油菜生物量估算研究[J]. 作物学报, 2021, 47(9): 1816-1823.
[13]	王艳花, 刘景森, 李加纳. 整合GWAS和WGCNA筛选鉴定甘蓝型油菜生物产量候选基因[J]. 作物学报, 2021, 47(8): 1491-1510.
[14]	王娜, 白建芳, 马有志, 郭昊宇, 王永波, 陈兆波, 赵昌平, 张立平. 小麦lncRNA27195及其靶基因TaRTS克隆及表达分析[J]. 作物学报, 2021, 47(8): 1417-1426.
[15]	周步进, 李刚, 金刚, 周瑞阳, 刘冬梅, 汤丹峰, 廖小芳, 刘一丁, 赵艳红, 王颐宁. 利用红麻HcPDIL5-2a非全长基因创制雄性不育新种质[J]. 作物学报, 2021, 47(6): 1043-1053.

Metrics

Viewed

Full text

209

HTML			PDF

Just accepted	Online first	Issue	Just accepted	Online first	Issue
0	0	0	0	0	209

From	Others	local

Times	13	196
Rate	6%	94%

Abstract

431

Just accepted	Online first	Issue

0	0	431

From	Others	local

Times	54	378
Rate	12%	88%

Cited

Web of Science	Crossref	ScienceDirect	Search for Citations in Google Scholar >>


This page requires you have already subscribed to WoS.

Shared

Discussed