宁麦9号对其衍生品种的遗传贡献

doi:10.3724/SP.J.1006.2014.00830

摘要/Abstract

摘要：

宁麦9号是江苏省农业科学院选育的优质弱筋专用小麦品种，具有高产、稳产和广泛的适应性及抗小麦黄花叶病、赤霉病等特点，近年来已成为江苏淮南麦区小麦育种的重要亲本。为了解宁麦9号对新育成小麦品种的遗传贡献，利用170对SSR引物，对宁麦9号及其9个衍生品种进行全基因组扫描分析。共检测到471个等位变异位点，每对引物可检测1~6个，平均2.8个。UPGMA聚类分析表明，扬麦18与宁麦9号遗传距离最小，扬辐麦4与宁麦9号遗传距离最大。遗传相似系数表明，在人工选择的作用下，这些以宁麦9号为亲本育成品种的遗传背景一半以上来自宁麦9号。宁麦9号等位变异在其衍生材料中的分布比例因品种而异，且不同染色体间差异较大，但相同位点的等位变异比例在A、B、D染色体组间相近。全基因组SSR扫描分析发现，10个标记位点在宁麦9号和由其选育的9个新品种具有完全相同的扩增带型，其中9个位点与重要农艺性状相关，或与控制优良性状的基因紧密连锁。将宁麦9号与主要弱筋小麦生产品种宁麦13（宁麦9号系选）进行基因组比较，两者遗传相似系数达0.732，说明宁麦13与宁麦9号遗传背景高度相似，而且两品种在蛋白质含量和抗赤霉病位点相关标记位点高度一致，这一结果可以部分解释宁麦13同样具有的抗赤霉病与优质弱筋的优点。

关键词: 宁麦9号, 宁麦13, SSR标记, 聚类分析, 遗传贡献

Abstract:

Ningmai 9 is a soft wheat cultivar with high yield, wide adaption, and resistance to multiple diseases including Fusarium head blight, yellow mosaic virus, powdery mildew, and sharp eye spots, which serves as a backbone parent in the southern Huaihe River valley. This study aimed to disclose the genetic contribution of Ningmai 9 to its derivates using SSR markers. A total of 471 alleles were detected on 170 SSR loci, with 1–6 alleles per locus and an average of 2.8. The UPGMA cluster analysis showed that Yangmai 18 and Ningmai 9 were clustered together firstly with the smallest genetic disrtance, and Yangfumai 4 and Ningmai 9 were clustered together with the largest genetic distance. Genetic similarity coefficient showed that more than a half of loci transferred from Ningmai 9 to its derived varieties. The percentage of alleles shared between Ningmai 9 and its derived varieties differed greatly among 21 chromosomes, but the average percentage was similar among A, B, and D genomes. Ten markers had the same amplification banding pattern in Ningmai 9 and nine derived varieties, and nine of them were linked to the QTLs or genes associated with important agronomic traits as previous reports. In comparison of genetic background between Ningmai 9 and Ningmai 13 (a direct selection line from Ningmai 9), the genetic similarity coefficient was 0.732, suggesting that the genetic back ground of Ningmai 13 is similar to that of Ningmai 9. Genotyping data showed that the markers associated with protein content and resistance to Fusarium head blight in Ningmai 13 were in correspondence with those in Ningmai 9. These results may partially explain the reason for elite agronomic traits of Ningmai 13 similar to Ningmai9m, such as resistance to Fusarium head blight and soft wheat quality.

Key words: Ningmai 9, Ningmai 13, SSR, Clustering analysis, Genetic contribution

姜朋,陈小霖,张平平,张鹏,姚金保,马鸿翔. 宁麦9号对其衍生品种的遗传贡献[J]. 作物学报, 2014, 40(05): 830-837.

JIANG Peng,CHEN Xiao-Lin,ZHANG Ping-Ping,ZHANG Peng,YAO Jin-Bao,MA Hong-Xiang. Genetic Contribution of Wheat Variey Ningmai 9 to Its Derivates[J]. Acta Agron Sin, 2014, 40(05): 830-837.

参考文献

[1]姚金保, 马鸿翔, 张平平, 姚国才, 杨学明, 任丽娟, 张鹏, 周淼平. 小麦优良亲本宁麦9号的研究与利用. 核农学报, 2012, 26: 17–21

Yao J B, Ma H X，Zhang P P, Yao G C, Yang X M, Ren L J, Zhang P, Zhou M P. Research of wheat elite parent Ningmai 9 and its utilization. Acta Agric Nucl Sin, 2012, 26: 17–21 (in Chinese with English abstract)

[2]You G X, Zhang X Y, Wang L F. An estimation of the minimum number of SSR loci needed to reveal genetic relationships in wheat varieties: Information from 96 random accessions with maximized genetic diversity. Mol Breed, 2005, 14: 397–406

[3]王兰芬, Balfourier F, 郝晨阳, Exbrayat F, 董玉琛, 盖红梅, 张学勇. 欧洲与东亚小麦品种遗传多样性的比较分析. 中国农业科学, 2007, 40: 2667–2678

Wang L F, Balfourier F, Hao C Y, Exbrayat F, Dong Y C, Ge H M, Zhang X Y. Comparison of genetic diversity level between European and East-Asian wheat collections using SSR markers. Sci Agric Sin, 2007, 40: 2667–2678 (in Chinese with English abstract)

[4]李玮瑜, 张斌, 张嘉楠, 昌小平, 李润植, 景蕊莲. 利用关联分析发掘小麦自然群体旗叶叶绿素含量的优异等位变异. 作物学报, 2012, 38: 962–970

Li W Y, Zhang B, Zhang J N, Chang X P, Li R Z, Jing R L. Exploring elite alleles for chlorophyll content of flag leaf in natural population of wheat by association analysis. Acta Agron Sin, 2012, 38: 962–970 (in Chinese with English abstract)

[5]Hao C Y, Wang L F, Ge H M, Dong Y C, Zhang X Y. Genetic diversity and linkage disequilibrium in Chinese bread wheat (Triticum aestivum L.) revealed by SSR markers. PloS ONE, 2011, e6(2): e17279. DOI: 10.1371/journal.pone.0017279

[6]Hao C Y, Dong Y C, Wang L F, You G X, Zhang H N, Ge H M, Jia J Z, Zhang X Y. Genetic diversity and construction of core collection in Chinese wheat genetic resources. Chin Sci Bull, 2008, 53: 1518–1526

[7]孙慧敏, 张军, 赵团结, 盖钧镒. 亚洲地区中、外大豆品种幼苗期耐淹性与SSR标记的关联分析. 作物学报, 2010, 36: 1615–1623

Sun H M, Zhang J, Zhao T J, Gai J Y. Association analysis between submergence tolerance and SSR markers in domestic and foreign soybean cultivars in Asia. Acta Agron Sin, 2010, 36: 1615–1623 (in Chinese with English abstract)

[8]韩俊, 张连松, 李静婷, 石丽娟, 解超杰, 尤明山, 杨作民, 刘广田, 孙其信, 刘志勇. 小麦骨干亲本“胜利麦/燕大1817”杂交组合后代衍生品种遗传构成解析. 作物学报, 2009, 35: 1395–1404

Han J, Zhang L S, Li J T, Shi L J, Xie C J, You M S, Yang Z M, Liu G T, Sun Q X, Liu Z Y. Molecular dissection of core parental cross “Triumph/Yanda 1817” and its derivatives in wheat breeding program. Acta Agron Sin, 2009, 35: 1395–1404 (in Chinese with English abstract)

[9]盖红梅, 李玉刚, 王瑞英, 李振清, 王圣健, 高峻岭, 张学勇. 鲁麦14对山东新选育小麦品种的遗传贡献. 作物学报, 2012, 38: 954–961

Ge H M, Li Y G, Wang R Y, Li Z Q, Wang S J, Gao J L, Zhang X Y. Genetic contribution of Lumai 14 to novel wheat varieties developed in Shandong Province. Acta Agron Sin, 2012, 38: 954–961 (in Chinese with English abstract)

[10]徐鑫, 李小军, 李秀全, 杨欣明, 刘伟华, 高爱农, 李立会. 小麦骨干亲本"洛夫林10号"1BL/1RS在衍生品种中的遗传分析. 麦类作物学报, 2010, 30: 221–226

Xu X, Li X J, Li X Q, Yang X M, Liu W H, Gao A N, Li L H. Inheritance of 1BL/1RS of founder parent Lovrin 10 in its progeny. J Triticeae Crops, 2010, 30: 221–226 (in Chinese with English abstract)

[11]刘新伦, 司清林, 李琴琴, 王长有, 王亚娟, 张宏, 吉万全. 利用SSR标记分析小麦骨干亲本阿夫及衍生品种(系)的遗传多样性和变化趋势. 农业生物技术学报, 2012, 20: 983–995

Liu X L, Si Q L, Li Q Q, Wang C Y, Wang Y J, Zhang H, Ji W Q. SSR analysis of genetic diversity and temporal trends of the core wheat (Triticum aestivum L.) parent Funo and its derivative varieties (lines). J Agric Biotech, 2012, 20: 983–995 (in Chinese with English abstract)

[12]袁园园, 王庆专, 崔法, 张景涛, 杜斌, 王洪刚. 小麦骨干亲本碧蚂4号的基因组特异位点及其在衍生后代中的传递. 作物学报, 2010, 36: 9–16

Yuan Y Y, Wang Q Z, Cui F, Zhang J T, Du B, Wang H G. Specific loci in genome of wheat milestone parent Bima 4 and their transmission in derivatives. Acta Agron Sin, 2010, 36: 9–16 (in Chinese with English abstract)

[13]Andersen J R, Lubberstedt T. Functional markers in plants. Trends Plant Sci, 2003, 8: 554–560

[14]Somers D J, Isaac P, Edwards K. A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet, 2004, 109: 1105–1114

[15]庄巧生. 中国小麦品种改良及其系谱分析. 北京: 中国农业出版社, 2003. pp 10–13

Zhuang Q S. Chinese Wheat Improvement and Pedigree Analysis. Beijing: China Agriculture Press, 2003. pp 10–13 (in Chinese)

[16]姚金保, 姚国才, 杨学明, 马鸿翔, 张平平. 小麦收获指数遗传及其与农艺性状的相关分析. 江苏农业学报, 2008, 24: 5–10

Yao J B, Yao G C, Yang X M, Ma H X, Zhang P P. Inheritance of wheat harvest index and its correlations with agronomic traits. Jiangsu J Agric Sci, 2008, 24: 5–10 (in Chinese with English abstract)

[17]姚金保, 任丽娟, 张平平, 杨学明, 马鸿翔, 姚国才, 张鹏, 周淼平. 小麦产量构成因素的双列杂交分析. 核农学报, 2011, 25: 633–638

Yao J B, Ren L J, Zhang P P, Yang X M, Ma H X, Yao G C, Zhang P, Zhou M P. Diallel analysis for yield components of wheat. Acta Agric Nucl Sin, 2011, 25: 633–638 (in Chinese with English abstract)

[18]姚金保, 张平平, 任丽娟, 杨学明, 马鸿翔, 姚国才, 张鹏, 周淼平. 软质冬小麦品种籽粒蛋白质含量的遗传分析. 江苏农业学报, 2011, 27: 469–474

Yao J B, Zhang P P, Ren L J, Yang X M, Ma H X, Yao G C, Zhang P, Zhou M P. Inheritance of grain protein content in soft red wheat cultivars. Jiangsu J Agric Sci, 2011, 27: 469–474 (in Chinese with English abstract)

[19]张平平, 姚金保, 马鸿翔. 小麦溶剂保持力的遗传分析. 江苏农业学报, 2010, 26: 1170–1175

Zhang P P, Yao J B, Ma H X. Genetic analysis of solvent retention capacity in wheat. Jiangsu J Agric Sci, 2010, 26: 1170–1175 (in Chinese with English abstract)

[20]姚金保, 任丽娟, 张平平, 杨学明, 马鸿翔, 姚国才, 张鹏, 周淼平. 小麦赤霉病的抗性遗传分析. 麦类作物学报, 2011, 31: 370–375

Yao J B, Ren L J, Zhang P P, Yang X M, Ma H X, Yao G C, Zhang P, Zhou M P. Genetic analysis of resistance to Fusarium head blight in wheat. J Triticeae Crops, 2011, 31: 370–375 (in Chinese with English abstract)

[21]周延清. DNA分子标记技术在植物研究中的应用. 北京: 化学工业出版社, 2005. pp 174–178

Zhou Y Q. Application of DNA Markers in Plant Research. Beijing: Chemical Industry Press, 2005. pp 174–178 (in Chinese)

[22]Liu S, Pumphrey M O, Gill B S, Trick H N, Zhang J X, Doleze J, Chalhoub B, Anderson J A. Toward positional cloning of Fhb1, a major QTL for Fusarium head blight resistance in wheat. Cereal Res Commun, 2008, 36(suppl-B): 195–201

[23]张平平, 马庆, 钱存鸣, 马鸿翔. 宁麦13选系的遗传多样性及品质差异. 作物学报, 2008, 34: 1484–1488

Zhang P P, Ma Q, Qian C M, Ma H X. Genetic diversity and quality difference of systemic selective lines in Ningmai 13. Acta Agron Sin, 2008, 34: 1484–1488 (in Chinese with English abstract)

[24]徐冠仁. 核农学导论. 北京:原子能出版社, 1997. pp 104–106

Xu G R. Introduction of Nuclear Agricultural Science. Atomic Energy Press, 1997. pp 104–106 (in Chinese)

[25]Roy J K, Bandopadhyay R, Rustgi S, Balyan H S, Gupta P K. Association analysis of agronomically important traits using SSR, SAMPL and AFLP markers in bread wheat. Curr Sci India, 2006, 90: 683–689

[26]Yao J, Wang L X, Liu L H, Zhao C P, Zheng Y L. Association mapping of agronomic traits on chromosome 2A of wheat. Genetica, 2009, 137: 67–75

[27]周晓果, 景蕊莲, 郝转芳, 昌小平, 张正斌. 小麦幼苗根系性状的QTL分析. 中国农业科学, 2005, 38: 1951–1957

Zhou X G, Jing R L, Hao C F, Chang X P, Zhang Z B. Mapping QTL for seedling root traits in common wheat. Sci Agric Sin, 2005, 38: 1951–1957 (in Chinese with English abstract)

[28]Ellis M H, Rebetzke G J, Azanza F, Richards R A, Spielmeyer W. Molecular mapping of gibberellin-responsive dwarfing genes in bread wheat. Theor Appl Genet, 2005, 111: 423–430

[29]Li Y, Song Y, Zhou R, Branlard G, Jia J. Detection of QTLs for bread-making quality in wheat using a recombinant inbred line population. Plant Breed, 2009, 128: 235–243

[30]Annapurnalilly B, Balgounda H, Mohinder P, Subhash B, Suryaprakasa R, Shubhada T. Validation and identification of molecular markers linked to the leaf rust resistance gene Lr28 in wheat. J Appl Genet, 2011, 52: 171–175

[31]Yang Z, Gilbert J, Fedak G, Somers D J. Genetic characterization of QTL associated with resistance to Fusarium head blight in a doubled-haploid spring wheat population. Genome, 2005, 48: 187–196

[32]景蕊莲, 昌小平, 贾继增. 一个小麦不孕小穗数QTL的染色体7A的作图区间Xgwm260-WMC83-WMC301. 中国专利号, CN200310115364.6. 2005-05-25

Jing R L, Chang X P, Jia J Z. Mapping interval Xgwm260-WMC83-WMC301 harboring a QTL for sterile spikelet number on chromosome 7A of wheat: China’s Patent No. CN200310115364.6. 2005-05-25 (in Chinese)

[33]Clarke F R, Knox R E, Clarke J M, Pozniak C J. Quantitative Trait Loci for agronomic, pest resistance and end-use quality traits in a durum wheat doubled haploid population. In: Molina-Cano J L, Christou P, Graner A, Hammer K, Jouve N, Keller B, Lasa J M, Powell W, Royo C, Shewry P, Stanca A M, eds. Cereal Science and Technology for Feeding Ten Billion People: Genomics Era and Beyond. Zaragoza: CIHEAM/IRTA, 2008. pp 111–113

[34]Jaiswal V, Mir R R, Mohan A, Balyan H S, Gupta P K. Association mapping for pre-harvest sprouting tolerance in common wheat (Triticum aestivum L.). Euphytica, 2012, 188: 89–102

[35]Lin F, Xue S L, Zhang Z Z, Zhang C Q, Kong Z X, Yao G Q, Tian D G, Zhu H L, Li CJ, Cao Y, Wei J B, Luo Q Y, Ma Z Q. Mapping QTL associated with resistance to Fusarium head blight in the Nanda 2419×Wangshuibai population: II: Type I resistance. Theor Appl Genet, 2006, 112: 528–535

[36]Groos C, Robert N, Bervas E, Charmet G. Genetic analysis of grain protein content, grain yield and thousand-kernel weight in bread wheat. Theor Appl Genet, 2003, 106: 1032–1040

[37]Prasad M, Kumar N, Kulwal P, Röder M, Balyan H, Dhaliwal H, Gupta P. QTL analysis for grain protein content using SSR markers and validation studies using NILs in bread wheat. Theor Appl Genet, 2003, 106: 659–667

[38]张小村, 李斯深, 赵新华, 范玉顶, 李瑞军. 小麦纹枯病抗性的QTL分析和抗病基因的分子标记, 植物遗传资源学报, 2005, 6: 276–279

Zhang X C, Li S S, Zhao X H, Fan Y D, Li R J. QTL and molecular markers for resistance gene of wheat sharp eyespot. J Plant Genet Res, 2005, 6: 276–279 (in Chinese with English abstract)

Metrics

Viewed

Full text

539

HTML			PDF

Just accepted	Online first	Issue	Just accepted	Online first	Issue
0	0	0	0	0	539

From	Others	local

Times	10	529
Rate	2%	98%

Abstract

261

Just accepted	Online first	Issue

0	0	261

From	Others	local

Times	64	197
Rate	25%	75%

Cited

Web of Science	Crossref	ScienceDirect	Search for Citations in Google Scholar >>


This page requires you have already subscribed to WoS.

Shared

Discussed