ATP合成相关基因在小麦BNS不育系育性转换中的差异表达

doi:10.3724/SP.J.1006.2014.01501

摘要/Abstract

摘要：

为了探讨ATP合酶α亚基和腺嘌呤磷酸核糖基转移酶(APRT)与温敏雄性不育系BNS育性的联系，利用荧光实时定量PCR方法，在花药发育的4个重要时期(四分体期、单核期、二核期和三核期)，定量检测ATP合酶α亚基和APRT相关基因在不育及可育条件下花药中的mRNA表达水平。不育条件下，ATP合酶α亚基基因从四分体到二核期表达量持续下降，与可育株相比在单核期表达量显著下降；APRT1在4个时期的表达量低于其在相应可育条件下的表达量，而APRT2基因在BNS不育和可育条件下维持较低的表达水平。APRT相关基因表达量在三核期均有较显著提高，且可育条件下比不育条件下提高更明显。因此认为，ATP合酶α亚基基因与BNS育性转换密切正相关，APRT基因在三核期转录水平的变化与BNS育性转换有一定关系。

关键词: 小麦, BNS雄性不育, ATP合酶α亚基, 腺嘌呤磷酸核糖基转移酶, 荧光实时定量PCR

Abstract:

BNS male sterile line is a temperature-sensitive type in wheat. This study aimed at disclosing the role of ATPase α submit and the adenine phosphoribosyl transferase (APRT) in BNS male sterility. At four key stages of pollen development, the differential expressions of ATPase α submit gene and APRT were analyzed using fluorescence real-time quantitative PCR (qRT-PCR) technique. Under sterile condition, the relative expression of ATPase α submit gene decreased constantly from tetrad phase to binucleated stage. Particularly, the expression of ATPase α submit gene in uninucleated period was significantly lower in sterile plant than in fertile plant. At four stages, APRT1 had lower expressions under sterile condition than under fertile condition, whereas, APRT2 maintained low expression level in fertility conversion. The transcription levels of APRT genes increased significantly in trinucleate period, and the variation was more obvious in fertile plants than in sterile plants. Therefore, ATPase α submit is positively related to fertility conversion in BNS sterile line and APRT genes probably play a role in trinucleate period.

Key words: Wheat, BNS male sterility, ATPase &alpha, submit, Adenine phosphoribosyl transferase, qRT-PCR.

王震,范晓静,张淼,张芳凝,李桂东,马翎健*. ATP合成相关基因在小麦BNS不育系育性转换中的差异表达[J]. 作物学报, 2014, 40(08): 1501-1505.

WANG Zhen,FAN Xiao-Jing,ZHANG Miao,LI Xue-Yin,ZHANG Fang-Ning,LI Gui-Dong,SHEN Fang-Di,MA Ling-Jian*. Differential Expression of ATP Synthesis Related Gene in Fertility Conversion of Wheat BNS Male Sterile Line[J]. Acta Agron Sin, 2014, 40(08): 1501-1505.

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