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作物学报 ›› 2014, Vol. 40 ›› Issue (09): 1557-1564.doi: 10.3724/SP.J.1006.2014.01557

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

小麦慢白粉病QTL对条锈病和叶锈病的兼抗性

刘金栋1,陈新民1,何中虎1,2,伍玲3,白斌4,李在峰5,夏先春1,*   

  1. 1中国农业科学院作物科学研究所/国家小麦改良中心,北京100081;2 CIMMYT中国办事处,北京100081;3四川省农业科学院作物研究所,四川成都 610066;4甘肃省农业科学院小麦研究所,甘肃兰州730070;5河北农业大学植物保护学院植物病理系,河北保定071001
  • 收稿日期:2014-02-05 修回日期:2014-06-16 出版日期:2014-09-12 网络出版日期:2014-07-09
  • 通讯作者: 夏先春, E-mail: xiaxianchun@caas.cn, Tel: 010-82108610
  • 基金资助:

    本研究由国家重点基础研究发展计划项目(2013CB127700), 国家自然科学基金项目(31261140370), 国家高新技术研究发展计划(863计划)项目(2012AA101105)和国家现代农业产业技术体系建设专项(CARS-3-1-3)资助。

Resistance of Slow Mildewing Genes to Stripe Rust and Leaf Rust in Common Wheat

LIU Jin-Dong1,CHEN Xin-Min1,HE Zhong-Hu1,2,WU Ling3,BAI Bin4,LI Zai-Feng5,XIA Xian-Chun1,*   

  1. 1 Institute of Crop Science/ National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China; 2CIMMYT-China Office, c/o CAAS, Beijing 100081, China; 3Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China; 4Wheat Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China; 5Department of Plant Pathology, College of Plant Protection, Agricultural University of Hebei, Baoding 071001, China
  • Received:2014-02-05 Revised:2014-06-16 Published:2014-09-12 Published online:2014-07-09
  • Contact: 夏先春, E-mail: xiaxianchun@caas.cn, Tel: 010-82108610

摘要:

聚合兼抗白粉病、条锈病和叶锈病的慢病性基因,是培育持久多抗小麦品种的重要措施。百农64和鲁麦21均为慢白粉病品种,分别含有4个和3个慢白粉病抗性QTL。将百农64与鲁麦21杂交,获得21个聚合2~5个慢白粉病抗性QTLF6株系,于2012—2013年度分别在四川郫县和甘肃天水进行条锈病田间抗性鉴定,在河北保定和河南周口进行叶锈病田间抗性鉴定。分析21个株系条锈和叶锈病的最大严重度和病程曲线下面积,检测单个QTLQTL聚合体对条锈病和叶锈病的抗性效应。结果表明,QPm.caas-4DLQPm.caas-6BSQPm.caas-2BL对条锈病均有显著的抗性,分别解释表型变异的16.9%14.1%17.3%QPm.caas-4DL对叶锈病也有显著抗性,可解释表型变异的35.3%QPm.caas-1A/QPm.caas-4DL/ QPm.caas-2DL/QPm.caas-2BS/QPm.caas-2BLQPm.caas-1A/QPm.caas-4DL/QPm.caas-2BS/QPm.caas-2BL聚合体对条锈病和叶锈病的抗性显著高于两亲本,它们均含有来自百农64QPm.caas-4DL以及来自鲁麦21QPm.caas-2BLQPm.caas-2BS,表明这些QTL具有明显的兼抗性效应。在小麦抗病育种中,聚合慢病性QTL越多,慢病性越强,聚合4~5个慢病性QTL时,株系可达到高抗甚至接近免疫的水平,是选育持久抗性小麦品种的重要手段。

关键词: 普通小麦, 慢病性, 持久抗性, 基因聚合, QTL

Abstract:

Pyramiding quantitative trait loci (QTLs) is an effective method to improve resistance to powdery mildew, stripe rust, and leaf rust in common wheat. We have developed 21 lines (F6) carrying 2-5 slow mildewing QTLs by crossing slow powdery mildew cultivars Bainong 64 and Lumai 21 possessing four and three slow mildewing QTLs, respectively. These F6 lines were evaluated in the field in Pianxian, Sichuan and Tianshui, Gansu for stripe rust resistance and in Baoding, Hebei and Zhoukou, Henan for leaf rust resistance during the 2012-2013 cropping season. According to the maximum disease severities (MDS) and the area under the disease progress curve (AUDPC), QTLs QPm.caas-4DL, QPm.caas-6BS and QPm.caas-2BL were highly resistant to stripe rust (P < 0.01), which explained 16.9%, 14.1%, and 17.3% of phenotypic variance, respectively. Locus QPm.caas-4DL also showed high resistance to leaf rust (P < 0.01) with phenotypic contribution of 35.3%. Lines that pyramided five (QPm.caas-1A/QPm.caas-4DL/ QPm.caas-2DL/QPm.caas-2BS/QPm.caas-2BL) and four (QPm.caas-1A/QPm.caas-4DL/QPm.caas-2BS/QPm.caas-2BL) QTLs exhibited higher resistance to both stripe and leaf rust compared with their parents. This result indicates that the combination of QPm.caas-4DL (from Bainong 64), QPm.caas-2BS and QPm.caas-2BL (Lumai 21) has a marked effect on improving adult resistance to powdery mildew, stripe rust and leaf rust, and the more QTLs are pyramided, the stronger slow disease resistance can be achieved. In breeding practice, the combination of 4-5 slow mildewing or rusting QTLs can result in durable resistance to multiple diseases.

Key words: Triticum aestivum L., Slow mildewing and slow rusting resistance, Durable resistance, Gene pyramiding, QTL

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