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作物学报 ›› 2014, Vol. 40 ›› Issue (09): 1549-1556.doi: 10.3724/SP.J.1006.2014.01549

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

玉米A亚族bZIP转录因子基因ZmbZIP81的克隆、表达与功能分析

王策1,2,杨艳歌2,3,吕维涛2,*,周春菊1,*,孙冬梅3,邓馨2   

  1. 1 西北农林科技大学生命科学学院，陕西杨凌712100；2 中国科学院植物研究所北方资源植物重点实验室，北京100093；3 黑龙江八一农垦大学生命科学技术学院，黑龙江大庆163319
  • 收稿日期:2014-01-21 修回日期:2014-06-16 出版日期:2014-09-12 网络出版日期:2014-06-27
  • 通讯作者: 吕维涛, E-mail: wtlv@ibcas.ac.cn, Tel: 13811773675; 周春菊, E-mail: zhchju@yahoo.com.cn
  • 基金资助:

    本研究由国家转基因生物新品种培育科技重大专项(2011ZX08009-002)资助。

Cloning, Expression, and Functional Analysis of an A Subfamily bZIP Transcription Factor Gene ZmbZIP81 in Maize

WANG Ce1,2,YANG Yan-Ge2,3,LÜ Wei-Tao2,*,ZHOU Chun-Ju1,*,SUN Dong-Mei3,DENG Xin2   

  1. 1 College of Life Science, Northwest Agriculture & Forestry University, Yangling 712100, China; 2 Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; 3 College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China
  • Received:2014-01-21 Revised:2014-06-16 Published:2014-09-12 Published online:2014-06-27
  • Contact: 吕维涛, E-mail: wtlv@ibcas.ac.cn, Tel: 13811773675; 周春菊, E-mail: zhchju@yahoo.com.cn

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摘要：

碱性亮氨酸拉链(basic leucine zipper, bZIP)蛋白是真核生物所特有的一类转录因子，对于植物在逆境下的基因表达调控具有重要作用。为丰富对玉米bZIP转录因子功能的认识，本研究以从玉米中克隆到的一个A亚族bZIP转录因子编码基因ZmbZIP81为对象展开。ZmbZIP81基因位于玉米第6染色体，编码区全长2492 bp，由4个外显子和3个内含子组成，编码蛋白含254个氨基酸。研究表明ZmbZIP81基因表达受外源ABA、NaCl和干旱胁迫诱导。过表达该基因的拟南芥植物表现出ABA不敏感和NaCl胁迫抗性增强的表型，推测ZmbZIP81可能作为ABA信号途径的负调控因子参与植物抗逆基因表达调控网络。

关键词: 玉米, bZIP转录因子, A亚族, ABA, 逆境胁迫

Abstract:

Basic leucine zipper (bZIP) proteins constitute a large family of transcription factors among eukaryotes, which plays important roles in gene expression regulation under abiotic stress in plants. In order to gain a better understanding of bZIPs in maize, we cloned ZmbZIP81 from maize, which encodes an A subfamily bZIP transcription factor. ZmbZIP81 is located on chromosome 6. The coding region of ZmbZIP81 is 2492 bp in length with four exons and three introns, which encodes 254 animo acid residues. Further study showed that the expression of ZmbZIP81 was induced by exogenous ABA, salt and drought treatments. The transgenic Arabidopsis plants overexpressing ZmbZIP81 were less sensitive to ABA, but more tolerant to salt, compared with the wild type. These results indicated that ZmbZIP81 may encode a transcription factor, regulate ABA signaling negatively and participate in abiotic stress tolerance regulation in maize.

Key words: Maize, bZIP transcription factor, A subfamily, ABA, Abiotic stress

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