作物学报 ›› 2014, Vol. 40 ›› Issue (09): 1565-1571.doi: 10.3724/SP.J.1006.2014.01565
姚姝,刘燕清,张亚东,朱镇,陈涛,赵庆勇,周丽慧,赵春芳,于新,王才林
YAO Shu,LIU Yan-Qing,ZHANG Ya-Dong,ZHU Zhen,CHEN Tao,ZHAO Qing-Yong,ZHOU Li-Hui,ZHAO Chun-Fang,YU Xin,WANG Cai-Lin*
摘要:
稻瘟病是我国水稻主产区的重要病害之一, 其主效抗性基因Pi-ta和Pi-b在我国很多稻区表现广谱持久的稻瘟病抗性, 被广泛应用于我国的水稻育种和生产。本研究选用稻瘟病抗性基因Pi-ta和Pi-b及其等位基因的功能标记, 在对22份分别已知抗病基因Pi-ta和Pi-b以及感病基因pi-ta与pi-b组成的水稻品种检测验证基础上, 建立了2套稻瘟病基因多重PCR体系: 体系I同时检测抗病基因Pi-ta与Pi-b, 体系II 同时检测感病基因pi-ta与pi-b, 并利用2套体系对336份高世代育种材料进行检测, 与单标记检测结果比较, 表现稳定可靠, 重复性好。本研究构建的抗稻瘟病基因分子标记多重PCR体系可用于水稻种质资源的快速评价和抗稻瘟病分子标记辅助育种。
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