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作物学报

作物学报 ›› 2014, Vol. 40 ›› Issue (10): 1767-1775.doi: 10.3724/SP.J.1006.2014.01767

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

花生属人工异源多倍体进化早期基因表达变化的cDNA-SCoT分析

贺梁琼1,2,3,熊发前1,唐秀梅1,蒋菁1,韩柱强1,钟瑞春1,高忠奎4,李忠1,何新华2,*,唐荣华1,*   

  1. 1广西农业科学院经济作物研究所，广西南宁 530007；2广西大学农学院，广西南宁 530004；3广西作物遗传改良生物技术重点开放实验室，广西南宁 530007；4广西农业科学院，广西南宁 530007
  • 收稿日期:2014-04-15 修回日期:2014-07-06 出版日期:2014-10-12 网络出版日期:2014-07-25
  • 通讯作者: 何新华, E-mail: honest66222@163.com; 唐荣华, E-mail: tronghua@163.com
  • 作者简介:何新华, E-mail: honest66222@163.com; 唐荣华, E-mail: tronghua@163.com
  • 基金资助:

    本研究由广西自然科学基金项目(2012GXNSFBA053051)，广西农业科学院科技发展基金项目(桂农科2012JM15)，国家自然科学基金项目(31160294，31240059)，广西科学研究与技术开发计划项目(桂科能14121008-1-4)，广西农业科学院基本科研业务专项项目(桂农科2014YZ05)和广西重点实验室建设项目(12-071-09)资助。

Analysis of Gene Expression Variation by cDNA-SCoT Technique at the Early Period of Arachis Artificial Allopolypoidy Evolution

HE Liang-Qiong1,2,3,XIONG Fa-Qian1,TANG Xiu-Mei1,JIANG Jing1,HAN Zhu-Qiang1,ZHONG Rui-Chun1,GAO Zhong-Kui4,Li Zhong1,HE Xin-Hua2,*,TANG Rong-Hua1,*   

  1. 1 Cash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; 2 Agricultural College of Guangxi University, Nanning 530004, China; 3 Guangxi Crop Genetic Improvement and Biotechnology Laboratory, Nanning 530007, China; 4 Guangxi Academy of Agricultural Sciences, Nanning 530007, China?
  • Received:2014-04-15 Revised:2014-07-06 Published:2014-10-12 Published online:2014-07-25
  • Contact: 何新华, E-mail: honest66222@163.com; 唐荣华, E-mail: tronghua@163.com
  • About author:何新华, E-mail: honest66222@163.com; 唐荣华, E-mail: tronghua@163.com

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被引次数

8

摘要：

为了探索花生属异源多倍体进化理论和种间杂交过程所涉及的遗传机制，以四倍体栽培种花生与二倍体野生种A. doigoi及其种间杂种F1和早期多倍体世代(S0~S3)为材料，采用cDNA-SCoT技术研究花生属人工异源多倍体进化早期基因表达变化规律。12条SCoT引物共扩增出108个cDNA片段，获得差异片段80个，占扩增总条带数的74.07%，对其中的35个差异片段进行克隆测序，有26个和GenBank数据库中已录入的基因具有较高的相似性，包括能量与代谢相关基因(8个)、未知功能蛋白基因(3个)、抗逆性相关基因(4个)、信号传导相关基因(2个)和反转录转座子相关基因(9个)。这说明花生属种间杂交人工异源多倍化早期世代发生着快速、剧烈的基因表达变化；从中获得的一些差异基因片段可用于花生属异源多倍化的分子机制研究。

关键词: 花生, 异源多倍化, cDNA-SCoT技术, 基因表达

Abstract:

To explore the allopolyploidy evolutionism and the genetic mechanism of Arachis interspecific hybridization, study the gene expression variation by cDNA-SCoT technique in the early period of Arachis artificial allopolypoidy evolution, using the progenitors, F1 and early polyploidy generations (S0 to S3) of the hybridization between tetraploid cultivated peanut and diploid wild peanut A. doigoi. Among 108 cDNA fragments amplified by 12 SCoT primers 80 were differentially expressed with the polymorphism frequency of 74.07%. Among 80 TDFs (transcripts derived fragments) 35 were cloned and sequenced, and the sequences of 26 TDFs shared high similarity with the genes documented in the GenBank. These genes included energy and metabolism-related genes (8), resistance-related genes (4), unknown functional protein genes (3), signal transduction-related genes (2) and retrotransposon-related genes (9). The results indicated that gene expression changes happened rapidly and drastically in the early generations during artificial allopolyploidization of peanut interspecific hybridization, and some obtained TDFs probably could be used in the research of molecular mechanism of Arachis allopolyploidization.

Key words: Peanut, Allopolyploidization, cDNA-SCoT technique, Gene expression

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