作物学报 ›› 2014, Vol. 40 ›› Issue (10): 1767-1775.doi: 10.3724/SP.J.1006.2014.01767
贺梁琼1,2,3,熊发前1,唐秀梅1,蒋菁1,韩柱强1,钟瑞春1,高忠奎4,李忠1,何新华2,*,唐荣华1,*
HE Liang-Qiong1,2,3,XIONG Fa-Qian1,TANG Xiu-Mei1,JIANG Jing1,HAN Zhu-Qiang1,ZHONG Rui-Chun1,GAO Zhong-Kui4,Li Zhong1,HE Xin-Hua2,*,TANG Rong-Hua1,*
摘要:
为了探索花生属异源多倍体进化理论和种间杂交过程所涉及的遗传机制,以四倍体栽培种花生与二倍体野生种A. doigoi及其种间杂种F1和早期多倍体世代(S0~S3)为材料,采用cDNA-SCoT技术研究花生属人工异源多倍体进化早期基因表达变化规律。12条SCoT引物共扩增出108个cDNA片段,获得差异片段80个,占扩增总条带数的74.07%,对其中的35个差异片段进行克隆测序,有26个和GenBank数据库中已录入的基因具有较高的相似性,包括能量与代谢相关基因(8个)、未知功能蛋白基因(3个)、抗逆性相关基因(4个)、信号传导相关基因(2个)和反转录转座子相关基因(9个)。这说明花生属种间杂交人工异源多倍化早期世代发生着快速、剧烈的基因表达变化;从中获得的一些差异基因片段可用于花生属异源多倍化的分子机制研究。
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